Researcher: Ayva, Çağla Ergün
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Ayva, Çağla Ergün
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Publication Metadata only TW68, cryptochromes stabilizer, regulates fasting blood glucose levels in diabetic ob/ob and high fat-diet-induced obese mice(Pergamon-Elsevier Science Ltd, 2023) Gul, Seref; Akyel, Yasemin Kubra; Ipek, Ozgecan Savlug; Akarlar, Busra Aytul; Taskin, Ali Cihan; Goren, Ahmet Ceyhan; Baris, Ibrahim; Ozturk, Nuri; Guzel, Mustafa; Aydin, Cihan; Okyar, Alper; Department of Molecular Biology and Genetics; Department of Molecular Biology and Genetics; Sürme, Saliha; Ayva, Çağla Ergün; Gül, Zeynep Melis; Özcan, Onur; Türkay, Metin; College of Sciences; Graduate School of Sciences and EngineeringCryptochromes (CRYs), transcriptional repressors of the circadian clock in mammals, inhibit cAMP production when glucagon activates G-protein coupled receptors. Therefore, molecules that modulate CRYs have the po-tential to regulate gluconeogenesis. In this study, we discovered a new molecule called TW68 that interacts with the primary pockets of mammalian CRY1/2, leading to reduced ubiquitination levels and increased stability. In cell-based circadian rhythm assays using U2OS Bmal1-dLuc cells, TW68 extended the period length of the circadian rhythm. Additionally, TW68 decreased the transcriptional levels of two genes, Phosphoenolpyruvate carboxykinase 1 (PCK1) and Glucose-6-phosphatase (G6PC), which play crucial roles in glucose biosynthesis during glucagon-induced gluconeogenesis in HepG2 cells. Oral administration of TW68 in mice showed good tolerance, a good pharmacokinetic profile, and remarkable bioavailability. Finally, when administered to fasting diabetic animals from ob/ob and HFD-fed obese mice, TW68 reduced blood glucose levels by enhancing CRY stabilization and subsequently decreasing the transcriptional levels of Pck1 and G6pc. These findings collectively demonstrate the antidiabetic efficacy of TW68 in vivo, suggesting its therapeutic potential for controlling fasting glucose levels in the treatment of type 2 diabetes mellitus.Publication Metadata only New biocompatible antibacterial wound dressing candidates; agar-locust bean gum and agar-salep films(Elsevier, 2020) Akkaya, Nil Erge; Saygun, Ayşe; Yesilçubuk, Neşe; Akel-Sadoğlu, Nurten; Türkmen, Halit S.; Çatalgil-Giz, Huceste; N/A; Department of Chemical and Biological Engineering; Department of Chemical and Biological Engineering; Ayva, Çağla Ergün; Kavaklı, İbrahim Halil; PhD Student; Faculty Member; Graduate School of Sciences and Engineering; College of Engineering; College of Engineering; N/A; 40319Agar has numerous applications in biomedical and biopharmaceutical fields in gel form. However the hard and tough nature of agar films and their vulnerability to microbial attacks prevent their usage in wound dressing applications. In this work, agar - locust bean gum (LBG) and agar - salep films were prepared for the first time to improve its physical, antimicrobial and cell viability properties. LBG and salep incorporated films resulted in higher antimicrobial and cell viability properties than agar films, which are very important in wound dressing applications. Agar - LBG films had higher water vapor permeabilities and were insoluble in water and in phosphate buffer solutions. Salep incorporation resulted in lower water vapor permeability and films were soluble in both media. All films were transparent, allowing good observability. With LBG and salep addition, lower tensile strength films were obtained and thicknesses of all films were appropriate for wound dressing applications. Due to their solubility, agar - salep films can be preferred especially for the cases where removal from the wound without damaging the tissue structure is a priority. (C) 2020 Elsevier B.V. All rights reserved.