Department of Molecular Biology and Genetics2024-11-0920222666-166710.1016/j.xpro.2022.1011582-s2.0-85124088015https://hdl.handle.net/20.500.14288/3131The SARS-CoV-2 main protease of (Mpro) is an important target for SARS-CoV-2 related drug repurposing and development studies. Here, we describe the steps for structural characterization of SARS-CoV-2 Mpro, starting from plasmid preparation and protein purification. We detail the steps for crystallization using the sitting drop, microbatch (under oil) approach. Finally, we cover data collection and structure determination using serial femtosecond crystallography.pdfX-Ray laserCrystallographySerialsJournal Articlehttps://doi.org/10.1016/j.xpro.2022.101158N/ANOIR03558