2024-11-092016978-1-4939-3055-5978-1-4939-3054-81064-374510.1007/7651_2015_227http://dx.doi.org/10.1007/7651_2015_227https://hdl.handle.net/20.500.14288/14220Neural stem cells (NSCs) derived from induced pluripotent stem cells offer therapeutic tools for neurodegenerative diseases. This review focuses on embryoid body (EB)-mediated stem cell culture techniques used to derive NSCs from mouse induced pluripotent stem cells (iPSCs). Generation of healthy and stable NSCs from iPSCs heavily depends on standardized in vitro cell culture systems that mimic the embryonic environments utilized during neural development. Specifically, the neural induction and expansion methods after EB formation are described in this review.Biochemical research methodsBiochemistryMolecular biologyGeneticsHeredityBook Chapter1940-60296963184000224333