2024-11-0920221064-374510.1007/7651_2021_4522-s2.0-85133525952http://dx.doi.org/10.1007/7651_2021_452https://hdl.handle.net/20.500.14288/6373Here, we describe a protocol for reprogramming of bone marrow–derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease. © 2021, Springer Science+Business Media, LLC.Induced pluripotent stem cellsNuclear reprogrammingCell differentiationGeneration and hematopoietic differentiation of mesenchymal stromal/stem cell-derived induced pluripotent stem cell lines for disease modeling of hematopoietic and immunological diseasesBook Chapterhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85133525952&doi=10.1007%2f7651_2021_452&partnerID=40&md5=195135b56eac77ced7a5087b905c97e53365