2025-01-1920230927-775710.1016/j.colsurfa.2023.1315712-s2.0-85154564959https://doi.org/10.1016/j.colsurfa.2023.131571https://hdl.handle.net/20.500.14288/26707Functional coatings have been of great interest in enhancing the performance of implants in recent years. Here, for the first time, preparation and characterization of a tetra-functional coating is reported, exhibiting anticorrosion, antibacterial, biocompatible, and anticoagulant behaviors. Poly(2-ethyl-2-oxazoline)-copolyethyleneimine (PEOX-co-PEI) stabilized silver nanoparticles (AgNPs) and heparin were used to prepare the multifunctional multilayers. The coatings were deposited on nitinol and 316 L stainless steel substrates, the most used materials in coronary stents, via layer-by-layer (LbL) technique. Corrosion current density values obtained from Tafel plots are 1.61 x 10-9 A/cm2 and 6.79 x 10-8A/cm2 for coated and uncoated 316 L substrate, respectively. Similarly, the corrosion current density values shifted from 1.00 x 10-8A/cm2 for uncoated nitinol to 4.77 x 10-10A/cm2 after coating. Contact killing method against gram-positive (Staphylococcus aureus) and gram-negative (Pseudomonas aeruginosa) bacterial species concluded that the presence of the coating on the surfaces of the materials reduces the number of the survived colonies (in CFU/mL) for more than 99.9%independent of the underlying metal within 3 hours of incubation time. Prepared coatings demonstrated above 90% relative viability compared to the positive control in MTT assay using human umbilical vein endothelial cells (HUVEC), indicating no cytotoxic effect on adjacent healthy cells. The coagulation assay also exhibited a 50% reduction in the coagulation rate after coating compared to the bare substrates, confirming the antithrombogenicity of the coatings.ChemistryPhysicalJournal Article1873-43591054963900001Q251142