KUVESG

Abstract

KUVESG, consists of mouse cortex images taken from cleared mouse brain samples with the 3DISCO procedure. Briefly, this organic solvent-based clearing protocol uses tetrahydrofuran for dehydration and dibenzyl ether (DBE) for matching the refractive index. After completing the protocol, 1 mm thick cleared brain slides in DBE solution were imaged with the confocal setup of the Leica multiphoton system TCS SP8 microscopy. The brain vasculature was visualized with premortem transcardial perfusion of gelatin-albumin-FITC solution (Sigma, A9771).