Research Project: Mitokondri Hedefli Aktive Edilebilir Kemilüminesan Ajanların Sentezi ve in vivo Timör Görüntüleme Uygulamaları
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Contributors
Funders
ID
TB.00501
Authors
Kölemen, Safacan
Faculty Member
Publications
A mitochondria-targeted chemiluminescent probe for detection of hydrogen sulfide in cancer cells, human serum and in vivo
(Royal Society of Chemistry, 2023) Acari, Alperen; Kölemen, Safacan; Gündüz, Hande; Dırak, Musa; Almammadov, Toghrul; KUYTAM (Koç University Surface Science and Technology Center); KUTTAM (Koç University Research Center for Translational Medicine); Department of Chemistry; Graduate School of Health Sciences; Graduate School of Sciences and Engineering; Yes; College of Sciences; GRADUATE SCHOOL OF HEALTH SCIENCES; GRADUATE SCHOOL OF SCIENCES AND ENGINEERING; Research Center
Hydrogen sulfide (H2S) as a critical messenger molecule plays vital roles in regular cell function. However, abnormal levels of H2S, especially mitochondrial H2S, are directly correlated with the formation of pathological states including neurodegenerative diseases, cardiovascular disorders, and cancer. Thus, monitoring fluxes of mitochondrial H2S concentrations both in vitro and in vivo with high selectivity and sensitivity is crucial. In this direction, herein we developed the first ever example of a mitochondria-targeted and H2S-responsive new generation 1,2-dioxetane-based chemiluminescent probe (MCH). Chemiluminescent probes offer unique advantages compared to conventional fluorophores as they do not require external light irradiation to emit light. MCH exhibited a dramatic turn-on response in its luminescence signal upon reacting with H2S with high selectivity. It was used to detect H2S activity in different biological systems ranging from cancerous cells to human serum and tumor-bearing mice. We anticipate that MCH will pave the way for development of new organelle-targeted chemiluminescence agents towards imaging of different analytes in various biological models.
