Research Project: Pi3,5P2 Sinyal İletiminin Mitoza Özgü Moleküler Etki Mekanizmalarının Çözümlenmesi
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Contributors
Funders
ID
TB.00728
Authors
Çaydaşı, Ayşe Koca
Faculty Member
Publications
PI(3,5)P2 asymmetry during mitosis is essential for asymmetric vacuolar inheritance
(Rockefeller University Press, 2025) Huda, Mariam; Koyuncu, Mukadder; Dilege, Cansu; Çaydaşı, Ayşe Koca; N/A; Department of Molecular Biology and Genetics; Graduate School of Sciences and Engineering; Yes; College of Sciences; GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling lipid that plays crucial roles in various cellular processes, including endolysosomal system structure/function, stress response, and cell cycle regulation. PI(3,5)P2 synthesis increases in response to environmental stimuli, yet its behavior in cycling cells under basal conditions remains elusive. Here, we analyzed spatiotemporal changes in PI(3,5)P2 levels during the cell cycle of S. cerevisiae. We found that PI(3,5)P2 accumulates on the vacuole in the daughter cell while it disappears from the vacuole in the mother cell during mitosis. Concomitant with the changes in PI(3,5)P2 distribution, the daughter vacuole became more acidic, whereas the acidity of the mother vacuole decreased during mitosis. Our data further showed that both PI(3,5)P2 and the PI(3,5)P2 effector protein Atg18 are determinants of vacuolar-pH asymmetry and acidity. Our work, thus, identifies PI(3,5)P2 as a key factor for the establishment of vacuolar-pH asymmetry, providing insights into how the mother cell ages while the daughter cell is rejuvenated.
