Research Project: Farklı iyonlaşma süreçlerinin test edilmesi ve kimyasal analiz amacı ile minyatür kütle spektrometri sistemi tasarımı ve prototip üretimi
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Contributors
Funders
ID
TB.00569
Authors
Kepçeoğlu, Abdullah
Researcher
Publications
Optimization of laser-wavelength dependence for open-air atmospheric pressure pulsed laser deposition of AlCrFeMnTi high-entropy alloy for tailored surface properties
(American Chemical Society, 2024)
Department of Chemistry
Graduate School of Sciences and Engineering
KUYTAM (Koç University Surface Science and Technology Center)
Yes
Alamdari, Armin Asghari
High-entropy alloys (HEAs) have garnered significant attention in different fields due to their exceptional mechanical and physical properties, making them promising candidates for various applications. Several techniques, including physical vapor deposition and pulsed laser deposition (PLD), have been employed for the fabrication of HEA thin films. In this study, we explore a novel approach to synthesizing the lightweight HEA (LWHEA) AlCrFeMnTi using PLD in air at atmospheric pressure with a particular focus on the influence of the laser wavelength on the deposition process and the resulting alloy characteristics. This research investigates the impact of different laser wavelengths on the LWHEA's characterization and the optimization of laser wavelength dependence in air at atmospheric pressure PLD of LWHEA AlCrFeMnTi for tailored surface properties such as phase composition, microstructure, and corrosion resistance. Systematically varying the laser wavelength was attempted to optimize the deposition conditions. This was aimed at achieving enhanced properties and precise control over the alloy's composition. This work contributes to a deeper understanding of the open air PLD process for LWHEAs and sheds light on the role of the laser wavelength in tailoring their properties, which can have significant implications for the development of advanced materials for aerospace, automotive, and other high-performance applications. Ultimately, this research aims to provide valuable insights into the design and fabrication of LWHEAs with tailored properties through laser-based deposition techniques.
Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source
(Nature Portfolio, 2023)
KUTTAM (Koç University Research Center for Translational Medicine)
Department of Molecular Biology and Genetics
KUYTAM (Koç University Surface Science and Technology Center)
Graduate School of Sciences and Engineering
School of Medicine
High-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 angstrom and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.
Comparative study of high-resolution LysB29(Nε-myristoyl) des(B30) insulin structures display novel dynamic causal interrelations in monomeric-dimeric motions
(Multidisciplinary Digital Publishing Institute (MDPI), 2023)
Kati, Ahmet
KUISCID (Koç University İşbank Center for Infectious Diseases)
Department of Molecular Biology and Genetics
Graduate School of Sciences and Engineering
Yes
The treatment of insulin-dependent diabetes mellitus is characterized by artificial supplementation of pancreatic beta-cell ability to regulate sugar levels in the blood. Even though various insulin analogs are crucial for reasonable glycemic control, understanding the dynamic mechanism of the insulin analogs may help to improve the best-protracted insulin analog to assist people with type 1 diabetes (T1D) to live comfortably while maintaining tight glycemic control. Here, we present the high-resolution crystal structure of NN304, known as insulin detemir, to 1.7 angstrom resolution at cryogenic temperature. We computationally further investigated our crystal structure's monomeric-dimeric conformation and dynamic profile by comparing it with a previously available detemir structure (PDB ID: 1XDA). Our structure (PDB ID: 8HGZ), obtained at elevated pH, provides electrostatically triggered minor movements in the equilibrium between alternate conformational substates compared to the previous structure, suggesting it might induce an intermediate state in the dissociation pathway of the insulin detemir's hexamer:dihexamer equilibrium. Supplemented with orientational cross-correlation analysis by a Gaussian network model (GNM), this alternate oligomeric conformation offers the distinct cooperative motions originated by loose coupling of distant conformational substates of a protracted insulin analog that has not been previously observed.