Publication:
Visualizing lipophagy as a new mechanism of the synthesis of sex steroids in human ovary and testis using immunofluorescence staining method

Placeholder

Organizational Units

Program

KU Authors

Co-Authors

Advisor

Publication Date

Language

en

Journal Title

Journal ISSN

Volume Title

Abstract

Immunofluorescence, a transformative tool in cellular biology, is employed to dissect the intricate mechanisms of cholesterol trafficking in human reproductive tissues. Autophagy, a key player in cellular homeostasis, particularly lipophagy, emerges as a free cholesterol source for steroidogenesis. In this chapter, we describe a comprehensive immunofluorescence staining protocol, with details provided for the precise visualization of subcellular dynamics of mitochondria, lysosomes, and lipid droplets in ex vivo testicular tissue and primary luteal granulosa cell culture models, pivotal components in sex steroid biosynthesis. Here, we detail the culture, treatment, and immunofluorescence protocols, providing a comprehensive guide for researchers. The provided immunofluorescence toolkit serves as a valuable resource for researchers, paving way for advancements in human reproductive health to investigate the intricate interplay between autophagy, lipophagy, and cholesterol trafficking.

Description

Source:

Methods Mol Biol

Publisher:

Springer

Keywords:

Subject

Medicine

Citation

Endorsement

Review

Supplemented By

Referenced By

Copy Rights Note

4

Views

0

Downloads

View PlumX Details