Determination of anaphase duration by time-lapse microscopy in budding yeast

Abstract

Time-lapse microscopy is a valuable, widely employed technique for investigating cell cycle dynamics. This chapter will guide you in using this tool to determine the duration of anaphase as a measure of mitotic exit kinetics in budding yeast cells. The methodology explained here is based on monitoring the mitotic spindle and provides a comprehensive guide covering all aspects, from sample preparation and microscopy to data analysis.