Publication: Label-free identification of exosomes using raman spectroscopy and machine learning
Program
KU-Authors
KU Authors
Co-Authors
Parlatan, Uğur
Özen, Mehmet Özgün
Keçoğlu, İbrahim
Koyuncu, Batuhan
Khalafkhany, Davod
Loc, Irem
Öğüt, Mehmet Giray
İnci, Fatih
Demir, Akın
Özören, Nesrin
Advisor
Publication Date
Language
English
Type
Journal Title
Journal ISSN
Volume Title
Abstract
Exosomes, nano-sized extracellular vesicles (EVs) secreted from cells, carry various cargo molecules reflecting their cells of origin. As EV content, structure, and size are highly heterogeneous, their classification via cargo molecules by determining their origin is challenging. Here, a method is presented combining surface-enhanced Raman spectroscopy (SERS) with machine learning algorithms to employ the classification of EVs derived from five different cell lines to reveal their cellular origins. Using an artificial neural network algorithm, it is shown that the label-free Raman spectroscopy method's prediction ratio correlates with the ratio of HT-1080 exosomes in the mixture. This machine learning-assisted SERS method enables a new direction through label-free investigation of EV preparations by differentiating cancer cell-derived exosomes from those of healthy. This approach will potentially open up new avenues of research for early detection and monitoring of various diseases, including cancer.
Description
Source:
Small
Publisher:
John Wiley and Sons Inc
Keywords:
Subject
Chemistry, Physics, Nanoscience, Nanotechnology, Materials Science, Condensed matter