Publication:
Point-of-care diagnostic platforms for loop-mediated isothermal amplification

dc.contributor.coauthorMunzer Alseed, Muhammad
dc.contributor.coauthorYetisen, Ali K.
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentDepartment of Mechanical Engineering
dc.contributor.departmentKUAR (KU Arçelik Research Center for Creative Industries)
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorAtçeken, Nazente
dc.contributor.kuauthorTaşoğlu, Savaş
dc.contributor.kuauthorDabbagh, Sajjad Rahmani
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2025-01-19T10:28:28Z
dc.date.issued2023
dc.description.abstractThe loop-mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle. With its unique primer design, LAMP ensures the rapid replication of the targeted DNA region with high specificity and high efficiency. LAMP technology is used for diagnostic purposes in pathogen detection due to its ease of use, low cost, and simplicity without requiring complex equipment. A wide range of LAMP diagnostic platforms have been developed for applications in bacteria, virus, and parasitic pathogen detection. Herein, the methodology of LAMP technology and its applications in pathogen detection and SNP genotyping and mutation detection are discussed. Point-of-care (PoC) LAMP platforms designed with the principles of microfluidic chip technology, including LAMP-on-a-chip, paper-based LAMP, and smartphone-based LAMP applications have been elaborated. LAMP technology represents a fast, robust, and reliable diagnostic platform for point-of-care testing.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.issue8
dc.description.openaccessAll Open Access; Hybrid Gold Open Access
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipFunding text 1: N.A. and M.M.A. contributed equally to this work. S.T. acknowledges Tubitak 2232 International Fellowship for Outstanding Researchers Award (118C391), Alexander von Humboldt Research Fellowship for Experienced Researchers, Marie Skłodowska-Curie Individual Fellowship (101003361), and Royal Academy Newton-Katip Çelebi Transforming Systems Through Partnership award (120N019) for financial support of this research. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the TÜBİTAK. This work was partially supported by Science Academy's Young Scientist Awards Program (BAGEP), Outstanding Young Scientists Awards (GEBİP), and Bilim Kahramanlari Dernegi The Young Scientist Award. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.; Funding text 2: N.A. and M.M.A. contributed equally to this work. S.T. acknowledges Tubitak 2232 International Fellowship for Outstanding Researchers Award (118C391), Alexander von Humboldt Research Fellowship for Experienced Researchers, Marie Skłodowska‐Curie Individual Fellowship (101003361), and Royal Academy Newton‐Katip Çelebi Transforming Systems Through Partnership award (120N019) for financial support of this research. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the TÜBİTAK. This work was partially supported by Science Academy's Young Scientist Awards Program (BAGEP), Outstanding Young Scientists Awards (GEBİP), and Bilim Kahramanlari Dernegi The Young Scientist Award. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
dc.description.volume25
dc.identifier.doi10.1002/adem.202201174
dc.identifier.issn1438-1656
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-85143914565
dc.identifier.urihttps://doi.org/10.1002/adem.202201174
dc.identifier.urihttps://hdl.handle.net/20.500.14288/25732
dc.identifier.wos898412100001
dc.keywordsLAMP-on-a-chip
dc.keywordsLoop-mediated isothermal amplification (LAMP)
dc.keywordsPaper-based LAMP
dc.keywordsPathogen detection
dc.keywordsPoint-of-care LAMP (PoC-LAMP) diagnostics
dc.keywordsSmartphone-based LAMP
dc.language.isoeng
dc.publisherJohn Wiley and Sons Inc
dc.relation.grantnoBilim Kahramanlari Dernegi; Royal Academy, (120N019); Alexander von Humboldt-Stiftung, AvH, (101003361); Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK; Bilim Akademisi
dc.relation.ispartofAdvanced Engineering Materials
dc.subjectMaterials science, multidisciplinary
dc.titlePoint-of-care diagnostic platforms for loop-mediated isothermal amplification
dc.typeReview
dspace.entity.typePublication
local.contributor.kuauthorAtçeken, Nazente
local.contributor.kuauthorDabbagh, Sajjad Rahmani
local.contributor.kuauthorTaşoğlu, Savaş
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Engineering
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Mechanical Engineering
local.publication.orgunit2KUAR (KU Arçelik Research Center for Creative Industries)
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2Graduate School of Sciences and Engineering
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