Publication:
Effect of vitamin D supplementation on OPG/RANKL signalling activities in endothelial tissue damage in diet-induced diabetic rat model

dc.contributor.coauthorÇelebi, Gizem
dc.contributor.coauthorAnapalı, Merve
dc.contributor.coauthorDağıstanlı, Fatma Kaya
dc.contributor.coauthorAkdemir, Ayşe Seda
dc.contributor.coauthorUlutin, Turgut
dc.contributor.coauthorKömürcü-Bayrak, Evrim
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorAydemir, Duygu
dc.contributor.kuauthorUlusu, Nuriye Nuray
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T22:56:52Z
dc.date.issued2022
dc.description.abstractBackground: Type 2 Diabetes Mellitus is a chronic metabolic disease that causes endothelial damage and is an important risk factor for atherosclerosis. In the present study vitamin D3 supplementation in rats was used to determine the role of Osteoprotegerin (OPG)/Receptor activator kB ligand (RANKL) signalling in endothelial damage and changes in the expression levels of genes involved in this pathway. We hypothesized that vitamin D3 supplementation affects OPG and RANKL activity in the aorta. Methods: Diabetes was induced in rats via injections of 40 mg/kg of streptozotocin followed by a high fructose (10%) diet. Group 2 (healthy) and 4 (diabetic) received 170 IU/kg of vitamin D3 weekly for 5 weeks, while Group 1 (healthy) and 2 (diabetic) received sterile saline. The aortas of each group were collected to analyse mRNA expression using the real-time PCR method and also to evaluate magnesium and calcium levels using inductively coupled plasma mass spectrometry. Results: Opg and Il-1b expression levels were significantly associated with both diabetes and vitamin D3 supplementation in the aortas of the study groups (p <= 0.05). Opg mRNA expression was also found to correlate with both Icam-1 and Nos3 mRNA expression levels (r = 0.699, p = 0.001 and r = 0.622, p = 0.003, respectively). In addition, when mineral levels in the aortic tissues were compared among all groups, it was found that the interaction of diabetes and vitamin D3 supplementation significantly affected Mg levels and Mg/Ca ratios. Conclusions: It is concluded that vitamin D3 supplementation has a modulatory effect on OPG/RANKL activity in the vessel wall by ameliorating endothelial damage in diabetes. This effect may contribute to the regulation of cytokine-mediated vascular homeostasis and mineral deposition in the aorta; therefore, further comprehensive studies are proposed to demonstrate this relationship.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University [TYL-2019-32715, TYL-2017-26139] This study was supported by the Scientific Research Projects Coordination Unit of Istanbul University (Project numbers: TYL-2019-32715 and TYL-2017-26139).
dc.description.volume74
dc.identifier.doi10.1007/s43440-021-00332-1
dc.identifier.eissn2299-5684
dc.identifier.issn1734-1140
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-85117072458
dc.identifier.urihttps://doi.org/10.1007/s43440-021-00332-1
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7456
dc.identifier.wos707679700001
dc.keywordsEndothelial damage
dc.keywordsOPG
dc.keywordsRANKL
dc.keywordsVitamin D3
dc.keywordsDiabetes coronary-artery-disease
dc.keywordsKappa-B ligand
dc.keywordsNitric-oxide
dc.keywordsVascular calcification
dc.keywordsReceptor activator
dc.keywordsIn-vitro
dc.keywordsOsteoprotegerin
dc.keywordsRANKL
dc.keywordsCells
dc.keywordsDysfunction
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofPharmacological Reports
dc.subjectPharmacology
dc.subjectPharmacy
dc.titleEffect of vitamin D supplementation on OPG/RANKL signalling activities in endothelial tissue damage in diet-induced diabetic rat model
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorAydemir, Duygu
local.contributor.kuauthorUlusu, Nuriye Nuray
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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