Publication:
SYBR green dye-based probe-free SNP genotyping: Introduction of T-Plex real-time PCR assay

dc.contributor.coauthorEtlik, Ozdal
dc.contributor.coauthorKoksal, Vedat
dc.contributor.coauthorOcak, Zeynep
dc.contributor.coauthorBaris, Saniye Tugba
dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.kuauthorBarış, İbrahim
dc.contributor.kuprofileTeaching Faculty
dc.contributor.yokid111629
dc.date.accessioned2024-11-10T00:11:17Z
dc.date.issued2013
dc.description.abstractSingle-nucleotide polymorphism (SNP) genotyping is widely used in genetic association studies to characterize genetic factors underlying inherited traits. Despite many recent advances in high-throughput SNP genotyping, inexpensive and flexible methods with reasonable throughput levels are still needed. Real-time PCR methods for discovering and genotyping SNPs are becoming increasingly important in various fields of biology. In this study, we introduce a new, single-tube strategy that combines the tetra-primer ARMS PCR assay, SYBR Green I-based real-time PCR, and melting-point analysis with primer design strategies to detect the SNP of interest. This assay, T-Plex real-time PCR, is based on the T. discrimination of the amplified allele-specific amplicons in a single tube. The specificity, sensitivity, and robustness of the assay were evaluated for common mutations in the FV, PII, MTHFR, and FGFR3 genes. We believe that T-Plex real-time PCR would be a useful alternative for either individual genotyping requests or large epidemiological studies.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue2
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume441
dc.identifier.doi10.1016/j.ab.2013.07.007
dc.identifier.issn0003-2697
dc.identifier.quartileN/A
dc.identifier.scopus2-s2.0-84884928258
dc.identifier.urihttp://dx.doi.org/10.1016/j.ab.2013.07.007
dc.identifier.urihttps://hdl.handle.net/20.500.14288/17460
dc.identifier.wos324605400022
dc.keywordsTetra-Primer arms PCR
dc.keywordsSNP
dc.keywordsFVL
dc.keywordsMthfr
dc.keywordsSybr Green
dc.keywordsT-Plex teal-time pcr Single nucleotide polymorphisms
dc.keywordsPolymerase-Chain-Reaction
dc.keywordsFactor-V-Leiden
dc.keywordsMelting temperature
dc.keywordsOligonucleotide probes
dc.keywordsProthrombin G20210a
dc.keywordsMutations
dc.keywordsDiagnosis
dc.keywordsCurves
dc.keywordsRFLP
dc.languageEnglish
dc.publisherElsevier
dc.sourceAnalytical Biochemistry
dc.subjectBiochemical research methods
dc.subjectBiochemistry
dc.subjectMolecular biology
dc.subjectChemistry, analytical
dc.titleSYBR green dye-based probe-free SNP genotyping: Introduction of T-Plex real-time PCR assay
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0003-2185-3259
local.contributor.kuauthorBarış, İbrahim
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Molecular Biology and Genetics
relation.isOrgUnitOfPublicationaee2d329-aabe-4b58-ba67-09dbf8575547
relation.isOrgUnitOfPublication.latestForDiscoveryaee2d329-aabe-4b58-ba67-09dbf8575547

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