Publication:
Real time visualization of cholesterol trafficking in human granulosa cells using confocal live cell microscopy as a tool to study the novel role of autophagy in sex steroid synthesis

Simple item page
dc.contributor.coauthorTürksen, Kürşad
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.facultymemberYes
dc.contributor.kuauthorEsmaeilian, Yashar
dc.contributor.kuauthorÖktem, Özgür
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2025-02-27T13:24:03Z
dc.date.issued2024
dc.description.abstractAutophagy is an evolutionarily conserved process that aims to maintain the energy homeostasis of the cell by recycling long-lived proteins and organelles. We have very recently demonstrated that lipophagy, a special form of autophagy, mediates the association of the lipid droplets (LDs) with lysosomes to deliver the lipid cargo within the LDs to lysosomes for degradation in order to release free cholesterol required for steroid synthesis in human ovary and testis. In this chapter, we describe live cell confocal microscopy technique that allows us to monitor real-time cholesterol trafficking and the association of cholesterol-laden LDs with lysosome (lipophagy) in human granulosa cells.
dc.description.fulltextNo
dc.description.harvestedfromManual
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessN/A
dc.description.peerreviewstatusN/A
dc.description.publisherscopeInternational
dc.description.readpublishN/A
dc.description.sponsoredbyTubitakEuN/A
dc.description.studentonlypublicationNo
dc.description.studentpublicationNo
dc.description.versionN/A
dc.identifier.WoSQuartileN/A
dc.identifier.doi10.1007/7651_2024_521
dc.identifier.eissn1940-6029
dc.identifier.embargoYes
dc.identifier.endpage150
dc.identifier.issn1064-3745
dc.identifier.pubmed38411890
dc.identifier.scopus2-s2.0-85219494078
dc.identifier.startpage139
dc.identifier.urihttps://hdl.handle.net/20.500.14288/26965
dc.identifier.urihttps://doi.org/10.1007/7651_2024_521
dc.identifier.volume2879
dc.language.isoeng
dc.publisherSpringer Nature
dc.relation.affiliationKoç University
dc.relation.collectionKoç University Institutional Repository
dc.relation.ispartofMethods in molecular biology
dc.relation.openaccessNo
dc.rightsCopyrighted
dc.subjectMolecular biology
dc.titleReal time visualization of cholesterol trafficking in human granulosa cells using confocal live cell microscopy as a tool to study the novel role of autophagy in sex steroid synthesis
dc.typeBook Chapter
dspace.entity.typePublication
local.contributor.kuauthorÖktem, Özgür
local.contributor.kuauthorEsmaeilian, Yashar
relation.isOrgUnitOfPublicationd02929e1-2a70-44f0-ae17-7819f587bedd
relation.isOrgUnitOfPublication91bbe15d-017f-446b-b102-ce755523d939
relation.isOrgUnitOfPublication.latestForDiscoveryd02929e1-2a70-44f0-ae17-7819f587bedd
relation.isParentOrgUnitOfPublication17f2dc8e-6e54-4fa8-b5e0-d6415123a93e
relation.isParentOrgUnitOfPublicationd437580f-9309-4ecb-864a-4af58309d287
relation.isParentOrgUnitOfPublication.latestForDiscovery17f2dc8e-6e54-4fa8-b5e0-d6415123a93e

Files

Collections

Publications without Fulltext