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Permanent URI for this collectionhttps://hdl.handle.net/20.500.14288/3
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Publication Metadata only Evidence of mitophagy in lens capsule epithelial cells of patients with pseudoexfoliation syndrome(Lippincott Williams and Wilkins, 2024) N/A; Aydemir, Dilara; Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Gözel, Merve; Karslıoğlu, Melisa Zişan; Güleser, Ümit Yaşar; Şahin, Afsun; Hasanreisoğlu, Murat; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; School of Medicine; Koç University HospitalPurpose Pseudoexfoliation Syndrome (PEX) is a condition in which aberrant fibrillary protein builds up in various components of the eye and other extraocular tissues. In this study, we aim to investigate the functionality of intracellular auto-degradative machinery -especially mitophagy- and related genes and proteins in PEX. Methods Anterior lens capsules were obtained from cataracts patients with and without PEX to constitute the PEX group and age-matched controls during microincision cataracts surgery. PINK1-mediated mitophagy markers were evaluated on the transcriptional and translational level via RT-qPCR and immunohistochemistry analysis, respectively. Results The lens epithelial cells of PEX patients were characterized by significantly higher PINK1 gene expression compared to that of the controls (p<0.05). In terms of intensity of staining of expressed proteins, PINK1 (p<0.05), Parkin (p<0.01) and LC3B (p<0.01) were all statistically higher in PEX, compared to the controls. Conclusion Altered auto-degradative response -specifically mitophagy- is a component of increased oxidative stress in PEX patients. The role of this mechanism in emerging complications warrants further research.Publication Metadata only Indomethacin affects the inflammatory response via interaction with the rhoa-actin cytoskeleton in THP-1 cells(Istanbul University Press, 2023) Aldogan, Ebru Haciosmanoglu; Bulut, Seyma; Yapislar, Hande; Guncer, Basak; Bektas, Muhammet; Yöntem, Fulya Dal; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of MedicineObjective: Inflammation is a complex reaction present in numerous disorders. Indomethacin, a compound possessing an indoline core, is a Nonsteroidal Anti-Inflammatory Drug (NSAID) that is commonly prescribed for inflammation and pain. The actin network, plays a major role in cellular activities and it’s regulated by by Rho GTPases has important implications for cellular dynamics and orientation. In this research, we explore the effects of indomethacin on the inflammatory response as mediated via RhoA and pyrin inflammatory complexes using an inflammatory disease model with relation actin cytoskeleton. Materials and Methods: This study used Western blotting to examine the impact of indomethacin on the assembly processes related to the pyrin inflammasome complex and the RhoA signaling pathway in Lipopolysaccharide-stimulated THP-1 cells. Actin-indomethacin interaction was analyzed by Differential Scanning Fluorimetry (DSF). Results: We found that while the expression levels of pyrin decreased, phosphorylated-RhoA increased but overall RhoA levels did not change. The equilibrium dissociation constant (KD) for the G-actin-indomethacin complex was calculated to be 9.591± 1.608 ng/mL (R2= 0.8582) using ∆Tm measurements of indomethacin by DSF. Conclusion: Moreover, the effects of indomethacin on inflammation pathways may provide insight into the molecular mechanisms of pyrin inflammasome formation in various autoimmune diseases.Publication Metadata only A case of left testicular artery with high origin passing through a left renal vein fenestration(Via Medica Medical Publishers, 2024) Yılmaz, Ebru; Tatar, Cem; Keskin, Aleyna; Yalçın, Büşra; Gürses, İlke Ali; Graduate School of Health Sciences; School of MedicineBackground: Fenestrations of are extremely rare in the venous system, especially renal veins. This paper aims to present a case of left renal vein fenestration where a high origin testicular artery passes through it. Materials and Methods The variation was observed incidentally in a 74-year-old Caucasian male cadaver during routine retroperitoneal dissections for second year medical students. Results: A fenestration in the mid portion of the left renal vein was observed. The length and height of the fenestration was 23 and 3.6 millimeters, respectively. The left testicular artery passed through the fenestration and followed a normal course distal to the fenestration. Posterior to the left renal vein, the testicular artery originated from the lateral aspect of abdominal aorta, just caudal to the left renal artery. On the right side, the testicular artery had a similar high origin, and two renal arteries were present. No venous variations were observed on the right side. Conclusions: The long course of the left renal vein is a factor of preference for donor kidney selection. Uncommon variations of the left renal veins, such as fenestrations, might result in a change in surgical technique and would put the left donor kidney at risk of prolonged anastomosis time and lower survival rates.Publication Metadata only Duloxetine in the treatment of women with urinary incontinence: a systematic review and meta-analysis of efficacy data from randomized controlled clinical trials(Galenos, 2023) Murat Uçar; Naşide Mangır; Ömer Gülpınar; Cüneyd Özkürkçigil; Oktay Demirkesen; Tarcan, Tufan; School of MedicineDuloxetine is the only available agent for the medical treatment of stress urinary incontinence (SUI). In this systematic review, we analyzed the efficacy and safety of duloxetine treatment in women with SUI and stress-predominant mixed urinary incontinence (SPMUI). We searched the literature using OVID MEDLINE, Embase and ULAKBIM (Turkish database) databases for placebo-controlled studies on the use of duloxetine in women with SUI or SPMUI. Data on change in incontinence episode frequency (IEF), decrease in the number of continence pads used, increase in voiding interval (minute) and discontinuation rates due to adverse effects and lack of efficacy (%) were extracted. A total of 12 randomized controlled trials were included. Duloxetine treatment results in an 18% decrease in IEF and 16% decrease in the number of incontinence pads used compared to pre-treatment status. It also increases the time interval between the voids by 18 min. Duloxetine treatment was associated with higher treatment discontinuation rates compared with placebo. The reason for discontinuation was related to the side effects of the treatment rather than lack of efficacy. Duloxetine can be an effective treatment option in women with UI based on high-level evidence supporting its efficacy. Further studies with larger patient populations and longer durations of follow-up are required to assess its safety profile.Publication Metadata only Preoperative radiosurgical management of brain metastases: evidence and challenges(Exon Publication, 2023) Topkan, E.; Kucuk, A.; Pehlivan, B.; Şenyürek, Şükran; Sezen, Duygu; Durankuş, Nilüfer Kılıç; Akdemir, Eyüb Yaşar; Bölükbaşı, Yasemin; Selek, Uğur; School of Medicine; Koç University HospitalAbout 30% of all adult patients with solid tumors will develop brain metastases. The prognosis of patients with brain metastasis is poor, with a median overall survival of 4–7 months. Nevertheless, with efficient systemic and local therapies, some specific patient groups may experience longer survival times. Currently, the options for the management of brain metastasis include surgery, systemic chemotherapy, targeted therapies, stereotactic radiosurgery (SRS), postoperative stereotactic radiosurgery, whole-brain radiotherapy (WBRT), and their combination variants. Given the severe neurotoxic effects of WBRT, increased risk of radionecrosis, leptomeningeal dissemination after postoperative SRS, and the ineligibility of certain patients for SRS during the postoperative period (usually first 21 days), an active search for alternative treatment strategies for such patients ensued. It has been suggested that novel preoperative stereotactic radiosurgery, which has a lower risk of radionecrosis and leptomeningeal dissemination, would provide at least equivalent local control rates in this regard. The purpose of the current chapter is to outline the justification and available evidence for the novel preoperative stereotactic radiosurgery in the management of brain metastasis while accepting the paucity of related literature.Publication Metadata only Is meat-free diet related to anxiety, depression and disordered eating behaviors? a cross-sectional survey in a Turkish sample(İnönü Üniversitesi, 2023) Çoşar, Behçet; Zorbozan, Emine Yağmur; Şanlı, Sevda Gümüş; Şentük, Erman; Geniş, Erman; Şentürk, Begüm Güler; Erus, Suat; School of MedicineAim: The foods that we eat don’t just impact our physical health. The link between what we eat and how we feel has become a trending topic. However, knowledge on the effects of diet types on this relation is still limited. The first aim of this study is to reveal the possible link between diet types and eating behaviors, anxiety and depression. Second one is to predict possible variables (demographic, health-related and medical) which cause higher depression scores among individuals following a meat-free diet and an omnivore diet. Materials and Methods: A questionnaire-based survey cross-sectional study was conducted among individuals with a meat-free diet and an omnivore diet (N = 836 with a vegan or a vegetarian diet, N = 519 with an omnivore diet) using an online questionnaire. Demographic, health-related and medical characteristics, The Three-Factor Eating Questionnaire-R 21, Hospital Anxiety and Depression Scale were used to assess variables. Results: A meat-free diet group showed significantly lower anxiety and depression scores, lower cognitive restraint, lower emotional eating and lower uncontrolled eating than omnivore diet group. Shared predictors of depression were dissatisfaction with physical appearance, uncontrolled eating and smoking in both meat-free diet group and omnivore diet group. Lower cognitive restraint and lower education level were predictors of depression in only omnivore diet group. Conclusion: This study revealed that an omnivore diet may be more associated with anxiety, depression, and some disordered eating behaviors than a meat-free diet.Publication Metadata only Radiation therapy for lung cancer in elderly(Springer Science and Business Media Deutschland Gmbh, 2023) Topkan, Erkan; Pehlivan, Berrin; Kucuk, Ahmet; Selek, Uğur; Bölükbaşı, Yasemin; School of MedicineElderly cancer patients with non-small and small cell lung cancer are altogether underrepresented nearly in all benchmark clinical trials; however, the embodiment of elderly patients in clinical studies should be emphatically encouraged to construct a proof-based optimal treatment of this specific age group. As most retrospective studies reveal similar oncologic results in elderly lung cancer patients compared to younger counterparts when treated with stage-specific recommendations, concerted efforts to accurately identify qualified patients to be prescribed standard treatments should be motivated. Therefore, we counsel a baseline and continuing guidance of geriatric assessment to promote individualized care, support, and tailored treatment protocols to avoid under- and overtreatments in elderly lung cancer patients.Publication Metadata only Comment on: predictive value of health-related quality of life on radiotherapy-related toxicities in patients with head and neck cancer(Springer Science and Business Media Deutschland Gmbh, 2023) Somay, Efsun; Topkan, Erkan; Selek, Uğur; School of MedicineN/APublication Metadata only Comments on: carcinoma buccal mucosa treated with definitive hypofractionated accelerated radiotherapy: a retrospective analysis of treatment outcomes(Ovid Technologies, 2023) Somay, E.; Yilmaz, B.; Topkan, E.; Selek, Uğur; School of MedicineN/APublication Metadata only Definitions of radiation-induced trismus in head and neck cancer: current concepts and controversies(NCBI Bookshelf, 2023) Somay, E.; Yilmaz, B.; Topkan, E.; Kucuk, A.; Pehlivan, B.; Selek, Uğur; School of MedicineRadiation-induced trismus is a devastating side effect of radiotherapy in patients with head and neck cancers. It hampers daily activities like eating, speaking, chewing, swallowing, and oral hygiene routines. Radiation-induced trismus also negatively affects social interactions, psychological wellbeing, and lowers the quality of life of patients. The most common method for determining radiation-induced trismus is to measure the ‘maximum mouth opening’. Different cut-off values for maximum mouth opening have been employed in studies that assessed radiation-induced trismus, including 40 mm, 35 mm, 30 mm, and 20 mm. The impact and prevalence of radiation-induced trismus are not fully understood because there is no common and reliable objective measure to determine cut-off values of maximum mouth opening. Additionally, regardless of the pretreatment measures, a standard cut-off is applied to all patients, where a change may be substantial for one patient but not necessarily for another. These discrepancies may cause certain patients’ conditions to be overstated or understated, misdirecting their prophylactic or therapeutic interventions. This chapter highlights the current concepts and controversies of the definitions of radiation-induced trismus, and the possible challenges in managing radiation-induced trismus because of the varied definitions.