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    The endoplasmic reticulum-mitochondria encounter structure complex coordinates coenzyme q biosynthesis
    (Sage, 2019) Eisenberg-Bord, Michal; Tsui, Hui S.; Antunes, Diana; Fernández-Del-Río, Lucía; Bradley, Michelle C; Nguyen, Theresa; Rapaport, Doron; Clarke, Catherine F; Schuldiner, Maya; Department of Molecular Biology and Genetics; Dunn, Cory David; Other; Department of Molecular Biology and Genetics; College of Sciences; N/A
    Loss of the endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) complex that resides in contact sites between the yeast ER and mitochondria leads to impaired respiration; however, the reason for that is not clear. We find that in ERMES null mutants, there is an increase in the level of mRNAs encoding for biosynthetic enzymes of coenzyme Q6 (CoQ6), an essential electron carrier of the mitochondrial respiratory chain. We show that the mega complexes involved in CoQ6 biosynthesis (CoQ synthomes) are destabilized in ERMES mutants. This, in turn, affects the level and distribution of CoQ6 within the cell, resulting in reduced mitochondrial CoQ6. We suggest that these outcomes contribute to the reduced respiration observed in ERMES mutants. Fluorescence microscopy experiments demonstrate close proximity between the CoQ synthome and ERMES, suggesting a spatial coordination. The involvement of the ER-mitochondria contact site in regulation of CoQ6 biogenesis highlights an additional level of communication between these two organelles.