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Permanent URI for this communityhttps://hdl.handle.net/20.500.14288/2
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Publication Restricted Determining the effects of DOT1L interacting proteins on cellular reprogramming(Koç University, 2018) Çimen, Deniz Uğurlu; Önder, Tamer Tevfik; 0000-0002-2372-9158; Koç University Graduate School of Sciences and Engineering; Molecular Biology and Genetics; 42946Publication Open Access DNA methylation profiling identifies novel markers of progression in hepatitis B-related chronic liver disease(BioMed Central, 2016) Vatansever, Sezgin; Hardy, Timothy; Sarı, Aysegül Akder; Çakalağaoğlu, Fulya; Avcı, Arzu; Zeybel, Gemma Louise; Bashton, Matthew; Mathers, John C.; Ünsal, Belkis; Mann, Jelena; N/A; Department of Molecular Biology and Genetics; Zeybel, Müjdat; Karahüseyinoğlu, Serçin; Faculty Member; Faculty Member; Department of Molecular Biology and Genetics; School of Medicine; 214694; 110772Background: Chronic hepatitis B infection is characterized by hepatic immune and inflammatory response with considerable variation in the rates of progression to cirrhosis. Genetic variants and environmental cues influence predisposition to the development of chronic liver disease; however, it remains unknown if aberrant DNA methylation is associated with fibrosis progression in chronic hepatitis B. Results: To identify epigenetic marks associated with inflammatory and fibrotic processes of the hepatitis B-induced chronic liver disease, we carried out hepatic genome-wide methylation profiling using Illumina Infinium beadarrays comparing mild and severe fibrotic disease in a discovery cohort of 29 patients. We obtained 310 differentially methylated regions and selected four loci comprising three genes from the top differentially methylated regions: hypermethylation of HOXA2 and HDAC4 along with hypomethylation of PPP1R18 were significantly linked to severe fibrosis. We replicated the prominent methylation marks in an independent cohort of 102 patients by bisulfite modification and pyrosequencing. The timing and causal relationship of epigenetic modifications with disease severity was further investigated using a cohort of patients with serial biopsies. Conclusions: Our findings suggest a linkage of widespread epigenetic dysregulation with disease progression in chronic hepatitis B infection. Cpg methylation at novel genes sheds light on new molecular pathways, which can be potentially exploited as a biomarker or targeted to attenuate inflammation and fibrosis.Publication Restricted New therapeutic approaches targeting MLL-AF9 leukemias through epigenetic reprogramming(Koç University, 2020) Bulut, İpek; Ayhan, Ceyda Açılan; 0000-0002-8936-3267; Koç University Graduate School of Sciences and Engineering; Bio-Medical Sciences and Engineering; 219658Publication Restricted The role of chromatin modifying enzymes in fibroblast-to-hepatocyte direct lineage conversion(Koç University, 2018) Enüstün, Eray; Önder, Tamer Tevfik; 0000-0002-2372-9158; Koç University Graduate School of Sciences and Engineering; Molecular Biology and Genetics; 42946Publication Restricted The role of KDM3B in castration resistant prostate cancer(Koç University, 2018) Saraç, Hilal; Lack, Nathan Alan; 0000-0001-7399-5844; Koç University Graduate School of Sciences and Engineering; Molecular Biology and Genetics; 120842