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Quartile: search.filters.quartile.Q2Subject: search.filters.subject.Biomedical materials

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Now showing 1 - 4 of 4
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    An exploration of plastic deformation dependence of cell viability and adhesion in metallic implant materials
    (Elsevier, 2016) Gerstein, G.; Maier, H. J.; N/A; N/A; N/A; N/A; Department of Mechanical Engineering; Uzer, Benay; Toker, Sıdıka Mine; Cingöz, Ahmet; Önder, Tuğba Bağcı; Canadinç, Demircan; Researcher; PhD Student; Researcher; Faculty Member; Faculty Member; Department of Mechanical Engineering; N/A; Graduate School of Sciences and Engineering; Graduate School of Health Sciences; School of Medicine; College of Engineering; N/A; 255504; N/A; 184359; 23433
    The relationship between cell viability and adhesion behavior, and micro-deformation mechanisms was investigated on austenitic 316L stainless steel samples, which were subjected to different amounts of plastic strains (5%, 15%, 25%, 35% and 60%) to promote a variety in the slip and twin activities in the microstructure. Confocal laser scanning microscopy (CLSM) and field emission scanning electron microscopy (FESEM) revealed that cells most favored the samples with the largest plastic deformation, such that they spread more and formed significant filopodial extensions. Specifically, brain tumor cells seeded on the 35% deformed samples exhibited the best adhesion performance, where a significant slip activity was prevalent, accompanied by considerable slip-twin interactions. Furthermore, maximum viability was exhibited by the cells seeded on the 60% deformed samples, which were particularly designed in a specific geometry that could endure greater strain values. Overall, the current findings open a new venue for the production of metallic implants with enhanced biocompatibility, such that the adhesion and viability of the cells surrounding an implant can be optimized by tailoring the surface relief of the material, which is dictated by the micro-deformation mechanism activities facilitated by plastic deformation imposed by machining.
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    Electro-conductive silica nanoparticles-incorporated hydrogel based on alginate as a biomimetic scaffold for bone tissue engineering application
    (Taylor and Francis Ltd., 2023) Derakhshankhah, Hossein; Eskandani, Morteza; Vandghanooni, Somayeh; Jaymand, Mehdi; Department of Mechanical Engineering; N/A; Taşoğlu, Savaş; Nakhjavani, Sattar Akbar; Faculty Member; Researcher; Department of Mechanical Engineering; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); College of Engineering; N/A; 291971; N/A
    An innovative electrically conductive hydrogel was fabricated through the incorporation of silica nanoparticles (SiO2 NPs) and poly(aniline-co-dopamine) (PANI-co-PDA) into oxidized alginate (OAlg) as a biomimetic scaffold for bone tissue engineering application. The developed self-healing chemical hydrogel was characterized by FTIR, SEM, TEM, XRD, and TGA. The electrical conductivity and swelling ratio of the hydrogel were obtained as 1.7 × 10−3 S cm−1 and 130%, respectively. Cytocompatibility and cell proliferation potential of the developed scaffold were approved by MTT assay using MG-63 cells. FE-SEM imaging approved the potential of the fabricated scaffold for hydroxyapatite (HA) formation and bioactivity induction through immersing in SBF solution.
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    Investigation of neurovascular effects of marine-derived molecules in 3D micro frame co-culture model
    (Mary Ann Liebert, Inc., 2022) Polat, İrem; Özkaya, Ferhat Can; Lahloubd, Mohamed-Farid; Ebrahimd, Weaam; Sokullu, Emel; Faculty Member; N/A; School of Medicine; N/A; 163024; 57111
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    Molecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for elisa applications
    (Taylor & Francis) Alkanli, Suleyman Serdar; Yasar, Merve; Guven, Celal; Kahraman, M. Vezir; Apohan, Nilhan Kayaman; Aktas, Zerrin; Oncul, Mustafa Oral; Unlu, Ayhan; Akcakaya, Handan; Yöntem, Fulya Dal; Teaching Faculty; School of Medicine; 232576
    Plastic antibodies can be used for in vitro neutralization of biomacromolecules with different fragments due to their potential in separation, purification, chemical sensor, catalysis and drug production studies. These polymer nanoparticles with binding affinity and selectivity comparable to natural antibodies were prepared using functional monomer synthesis and copolymerization of acrylic monomers via miniemulsion polymerization. As a result, the in vitro cytotoxic effect from diphtheria toxin was reduced by MIPs. In vitro imaging experiments of polymer nanoparticles (plastic antibodies) were performed to examine the interaction of diphtheria toxin with actin filaments, and MIPs inhibited diphtheria toxin damage on actin filaments. The enzyme-linked immunosorbent assay (ELISA) was performed with plastic antibodies labeled with biotin, and it was determined that plastic antibodies could also be used for diagnostic purposes. We report that molecularly imprinted polymers (MIPs), which are biocompatible polymer nanoparticles, can capture and reduce the effect of diphtheria toxic and its fragment A.