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Now showing 1 - 10 of 24
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    Actin distribution in HepG2 cell-spheroids: a fluorescence microscopic study
    (Amer Soc Cell Biology, 2012) Karahuseyinoglu, S.; Tok, O. E.; Yucel, D.; Aktaş, Ranan Gülhan; Karabörk, Merve; Faculty Member; Undergraduate Student; School of Medicine; School of Medicine; 137519; 358512
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    Activation of toll-like receptor signaling in endothelial progenitor cells dictates angiogenic potential: from hypothesis to actual state
    (Springer, 2021) Avci, Cigir Biray; Saberianpour, Shirin; Ahmadi, Mahdi; Hassanpour, Mehdi; Bagheri, Hesam Saghaei; Rezaie, Jafar; Talebi, Mehdi; Roodbari, Fatemeh; Darabi, Masoud; Rahbarghazi, Reza; N/A; Heidarzadeh, Morteza; Sokullu, Emel; PhD Student; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; School of Medicine; N/A; 163024
    Human endothelial progenitor cells (EPCs) were isolated from cord blood samples and enriched by magnetic activated cell sorting method based on the CD133 marker. Cells were incubated with different doses of bacterial lipopolysaccharide, ranging from 2, 5, 10, 50, 100, 200, 250, 500, to 1000 mu g/ml, for 48 h. The cell survival rate was determined by using MTT assay. To confirm activation of the toll-like receptor signaling pathway, PCR array analysis was performed. Protein levels of ERK1/2, p-ERK1/2, NF-kappa B and TRIF proteins were measured using western blotting. The content of TNF-alpha and lipoprotein lipase activity were analyzed by immunofluorescence imaging. Flow cytometric analysis of CD31 was performed to assess the maturation rate. Cell migration was studied by the Transwell migration assay. The expression of genes related to exosome biogenesis was measured using real-time PCR analysis. In vivo gel plug angiogenesis assay was done in nude mice. Lipopolysaccharide changed endothelial progenitor cells' survival in a dose-dependent manner with maximum viable cells in groups treated with 2 mu g/ml. PCR array analysis showed the activation of toll-like signaling pathways after exposure to LPS (p<0.05). Western blotting analysis indicated an induction of p-ERK1/2 and Erk1/2, NF-kappa B and TRIF in LPS-treated EPCs compared with the control (p<0.05). Immunofluorescence staining showed an elevation of TNF-alpha and lipoprotein lipase activity after lipopolysaccharide treatment (p<0.05). Lipopolysaccharide increased EPC migration and expression of exosome biogenesis-related genes (p<0.05). In vivo gel plug analysis revealed enhanced angiogenesis in cells exposed to bacterial lipopolysaccharide. Data highlighted the close relationship between the toll-like receptor signaling pathway and functional activity in EPCs.
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    Application of exosomes for the alleviation of COVID-19-related pathologies
    (Wiley, 2022) Rezabakhsh, Aysa; Mahdipour, Mahdi; Nourazarian, Alireza; Habibollahi, Paria; Avcı, Çığır Biray; Rahbarghazi, Reza; Sokullu, Emel; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; 163024
    The pandemic of COVID-19 caused worldwide concern. Due to the lack of appropriate medications and the inefficiency of commercially available vaccines, lots of efforts are being made to develop de novo therapeutic modalities. Besides this, the possibility of several genetic mutations in the viral genome has led to the generation of resistant strains such as Omicron against neutralizing antibodies and vaccines, leading to worsening public health status. Exosomes (Exo), nanosized vesicles, possess several therapeutic properties that participate in intercellular communication. The discovery and application of Exo in regenerative medicine have paved the way for the alleviation of several pathologies. These nanosized particles act as natural bioshuttles and transfer several biomolecules and anti-inflammatory cytokines. To date, several approaches are available for the administration of Exo into the targeted site inside the body, although the establishment of standard administration routes remains unclear. As severe acute respiratory syndrome coronavirus 2 primarily affects the respiratory system, we here tried to highlight the transplantation of Exo in the alleviation of COVID-19 pathologies.
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    Changes on the expression of 84 key genes involved in the progression of hepatocellular carcinoma (HCC) during differentiation of HepG2 cells
    (Amer Soc Cell Biology, 2013) Aktaş, Ranan Gülhan; Önder, Tuğba Bağcı; Faculty Member; School of Medicine; 184359
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    Deep mutational scanning reveals characteristics important for mitochondrial targeting of a tail-anchored protein.
    (The American Society for Cell Biology, 2017) N/A; N/A; N/A; Department of Molecular Biology and Genetics; Keskin, Abdurrahman; Akdoğan, Emel; Dunn, Cory David; Master Student; Master Student; Other; Department of Molecular Biology and Genetics; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; N/A; N/A; N/A
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    Dynamic recruitment of the retinal degeneration gene product CCDC66 to the centrosome/cilium complex is regulated by satellites and nnicrotubules
    (Amer Soc Cell Biology, 2018) N/A; Department of Molecular Biology and Genetics; N/A; Karalar, Elif Nur Fırat; Çonkar, Deniz; Faculty Member; PhD Student; Department of Molecular Biology and Genetics; College of Sciences; Graduate School of Sciences and Engineering; 206349; N/A
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    Interconnections of fibroblast growth factor 23 and klotho with erythropoietin and hypoxia-inducible factor
    (Springer, 2022) Afsar, Baris; Afsar, Rengin Elsurer; N/A; Kanbay, Mehmet; Faculty Member; School of Medicine; 110580
    Bone marrow (BM) hematopoiesis is tightly regulated process and bone components such as osteoblasts, extracellular matrix, and minerals influence hematopoiesis via regulation of hematopoietic stem cell function. Erythropoietin (EPO) secreted mostly by renal EPO producing (REP) cells which employ the hypoxia-inducible factor (HIF) pathway. When tissue hypoxia occurs, HIFs bind to hypoxia response element in the EPO promoter and induce EPO production. EPO binds to the EPO receptor on red cell progenitors in the BM and triggers expansion of red cell mass. Fibroblast growth factor-23 (FGF23) which is secreted mostly by osteoblasts and less by BM impacts hematopoiesis by influencing EPO production. Reciprocally, increases of EPO (acute or chronic) influence both FG23 production and cleavage resulting in variation of c fragment FGF23 (cFGF23) and intact FGF23 (iFGF23) ratios. As HIFs stimulate EPO production, they indirectly affect FGF23. Direct stimulation of FGF23 synthesis by binding of HIF on FGF23 promoter is also suggested. FGF23 cleavage by furin is another potential mechanism affecting FGF23 levels. Klotho is present in membrane-bound (transmembrane) and free (circulating) forms. Transmembrane klotho is the co-receptor of FGF23 and forms complexes with FGF23 receptors in the membrane surface and required for FGF23 actions. Recent evidence showed that klotho is also associated with EPO and HIF production suggesting a complex relationship between FGF23, klotho, EPO, and HIF. In this review, we have summarized the connections between FGF23, klotho, HIF, and EPO and their reflections to hematopoiesis.
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    Mechanisms of the effects of erythropoietin in wound healing: a morphologic study
    (American Society for Cell Biology (ASCB), 2012) Oktayer, A. G.; Güven, A.; Ickin, M.; Ozacmak, H. S.; Ozacmak, V. H.; Tok, O. E.; N/A; Aktaş, Ranan Gülhan; Faculty Member; School of Medicine
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    Mechanisms of the effects of erythropoietin in wound healing: a morphologic study.
    (Amer Soc Cell Biology, 2012) Guven, A.; Ozacmak, H. S.; Ozacmak, V. H.; Ickin, M.; Tok, O. E.; Aktaş, Ranan Gülhan; Oktayer, Adviye Gözde; Faculty Member; Master Student; School of Medicine; Graduate School of Health Sciences; 137519; N/A
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    Mito tracker labeling in HepG2 cell cultures: influence of different staining techniques on the morphology of mitochondria.
    (Amer Soc Cell Biology, 2012) Karahuseyinoglu, S.; Tok, O. E.; Yucel, D.; Aktaş, Ranan Gülhan; Karabörk, Merve; Faculty Member; Undergraduate Student; School of Medicine; School of Medicine; 137519; 358512