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Publication Restricted Correlation between the mechanical and histological properties of liver tissue(Koç University, 2013) Yarpuzlu, Berkay; Başdoğan, Çağatay; 0000-0002-6382-7334; Koç University Graduate School of Sciences and Engineering; Mechanical Engineering; 125489Publication Restricted Design, fabrication and characterization of biodegradable scaffolds for tissue engineering and regenerative medicine applications(Koç University, 2018) Nazeer, Muhammad Anwaar; Yılgör, İskender; 0000-0002-7756-4192; Koç University Graduate School of Sciences and Engineering; Bio-Medical Sciences and Engineering; 24181Publication Restricted Design, fabrication and characterization of light-responsive functionalized hydrogel for tissue engineering applications(Koç University, 2021) Batool, Syeda Rubab; Kızılel, Seda; 0000-0001-9092-2698; Koç University Graduate School of Sciences and Engineering; Bio-Medical Sciences and Engineering; 28376
Publication Metadata only Growth and organotypic branching of lung-specific microvascular cells on 2D and in 3D lung-derived matrices(N/A, 2024) Özkan, Sena Nur; Öztürk, Ece; Graduate School of Health Sciences; School of MedicineTissue-specific endothelial cells have vital roles in maintenance and functioning of native tissues with constant reciprocal crosstalk with resident cells. Three-dimensional (3D) physio-mimetic in vitro models which incorporate lung-specific microvasculature are needed to model lung-related diseases which involve modulation of endothelial cell behavior like cancer. In this study, we investigated the growth kinetics, morphological changes and responses to biological cues of lung microvasculature on two-dimensional (2D) and in lung matrix-derived 3D hydrogels. HUVEC and HULEC-5a cells were cultured on 2D and compared for their growth, morphologies, and responses to varying growth medium formulations. Brightfield and immunofluorescence imaging was performed to assess differences in morphology. For 3D cultures, native bovine lungs were decellularized, lyophilized, solubilized, and reconstituted into hydrogel form in which endothelial cells were embedded. Cell growth and organotypic branching was monitored in 3D hydrogels in the presence of varying biological cues including lung cancer cell secretome. HUVEC and HULEC-5a cells demonstrated comparable growth and morphology on 2D. However, in 3D lung-derived ECM hydrogels, tissue-specific HULEC-5a cells exhibited much better adaptation to their microenvironment, characterized by enhanced organotypic branching and longer branches. HULEC-5a growth was responsive to lung cancer cell-conditioned medium in both 2D and 3D conditions. In 3D, the concentration of ECM ligand significantly affected cell growth in long-term culture where molecular crowding had an inhibitory role. Our data reveals that HULEC-5a cells offer a reliable alternative to frequently pursued HUVECs with comparable growth and morphology. Due to their intrinsic program for cellular crosstalk with resident cells, the use of tissue-specific endothelium constitutes a vital aspect for modeling physiological and pathological processes. Furthermore, our study is the first demonstration of the synergy between lung-specific microvasculature with lung-specific ECM within a 3D in vitro model.Publication Restricted Human growth factor production towards in vitro vascularization(Koç University, 2022) Tunalı, Beste; Albayrak, Cem; 0000-0002-4496-4237; Koç University Graduate School of Sciences and Engineering; Chemical and Biological EngineeringPublication Metadata only Investigation of neurovascular effects of marine-derived molecules in 3D micro frame co-culture model(Mary Ann Liebert, Inc., 2022) Polat, İrem; Özkaya, Ferhat Can; Lahloubd, Mohamed-Farid; Ebrahimd, Weaam; Sokullu, Emel; Faculty Member; N/A; School of Medicine; N/A; 163024; 57111N/APublication Metadata only Microphysiological modeling of the human endometrium(Mary Ann Liebert, Inc, 2020) Campo, Hannes; Murphy, Alina; Woodruff, Teresa; Cervello, Irene; Kim, J. Julie; N/A; Yıldız, Şule; Faculty Member; School of Medicine; 134205Since the beginning of clinical medicine, the human uterus has held the fascination of clinicians and researchers, given its critical role in the reproduction of our species. The endometrial lining provides residence for the embryo; however, this symbiotic interaction can be disrupted if the timing is not correct and the endometrium is not receptive. Diseases associated with the endometrium interfere with the reproductive process and cause a life-altering burden of pain and even death. With the advancement of technologies and new insights into the biology of the endometrium, much has been uncovered about the dynamic and essential changes that need to occur for normal endometrial function, as well as aberrations that lead to endometrial diseases. As expected, the more that is uncovered, the more the complexity of the endometrium is made evident. In this study, we bring together three areas of scientific advancement that remain in their infancy, but which together have the potential to mirror this complexity and enable understanding. Studies on induced pluripotent stem cells, three-dimensional tissue mimics, and microfluidic culture platforms will be reviewed with a focus on the endometrium. These unconventional approaches will provide new perspectives and appreciation for the elegance and complexity of the endometrium. Impact statement The ability of the human endometrium to regenerate on a monthly basis for similar to 4 decades of reproductive years exemplifies its complexity as well as its susceptibility to disease. Restrictions on the types of research that can be done in the human endometrium motivate the development of new technologies and model systems. The three areas of technological advancement reviewed here-induced pluripotent stem cells, three-dimensional model systems, and microfluidic culture systems-will highlight some of the tools that can be applied to studying the human endometrium in ways that have not been done before.Publication Restricted Novel 3D composite polymeric scaffolds for tissue engineering(Koç University, 2022) Atay, İpek; Yılgör, İskender; 0000-0002-7756-4192; Koç University Graduate School of Sciences and Engineering; Bio-Medical Sciences and Engineering; 24181Publication Metadata only Three-dimensional neurovascular co-culture inside a microfluidic invasion chemotaxis chip(Mary Ann Liebert, Inc, 2022) Cücük, Levent; Polat, İrem; N/A; Department of Mechanical Engineering; Sokullu, Emel; Taşoğlu, Savaş; Faculty Member; Faculty Member; Department of Mechanical Engineering; School of Medicine; College of Engineering; 163024; 291971N/A