Researcher:
Fidan, Kerem

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Master Student

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Kerem

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Fidan

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Fidan, Kerem

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2015 - 20162

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Researcher Of Publications: search.filters.isAuthorOfPublication.06f22f93-f1e9-4fde-8496-6b7f0951283f

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    Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells
    (Humana Press Inc, 2016) Ebrahimi, Ayyub; N/A; N/A; N/A; N/A; Fidan, Kerem; Bozlak, Özlem Hilal Çağlayan; Özçimen, Burcu; Önder, Tamer Tevfik; Master Student; Undergraduate Student; Phd Student; Faculty Member; Graduate School of Sciences and Engineering; School of Medicine; Graduate School of Sciences and Engineering; School of Medicine; N/A; 296433; 316273; 42946
    Induced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation.
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    PublicationOpen Access
    Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)
    (Elsevier, 2015) Gül, Ahmet; Department of Molecular Biology and Genetics; Fidan, Kerem; Kavaklıoğlu, Gülnihal; Ebrahimi, Ayyub A.; Özlü, Can; Ay, Nur Zeynep; Ruacan, Ayşe Arzu; Önder, Tamer Tevfik; Master Student; Faculty Member; Faculty Member; Department of Molecular Biology and Genetics; School of Medicine; Graduate School of Sciences and Engineering; N/A; N/A; N/A; N/A; N/A; 38250; 42946
    Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line.