Researcher:
Özdaş, Şule Beyhan

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Şule Beyhan

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Özdaş

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Özdaş, Şule Beyhan

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2006 - 200942010 - 20131

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    Discovery of novel CYP17 inhibitors for the treatment of prostate cancer with structure-based drug design
    (Bentham Science Publ Ltd, 2009) N/A; N/A; N/A; Department of Chemical and Biological Engineering; Department of Industrial Engineering; Armutlu, Pelin; Özdemir, Muhittin Emre; Özdaş, Şule Beyhan; Kavaklı, İbrahim Halil; Türkay, Metin; Master Student; Master Student; Researcher; Faculty Member; Faculty Member; Department of Chemical and Biological Engineering; Department of Industrial Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Engineering; College of Engineering; N/A; N/A; N/A; 40319; 24956
    It has been shown that prostate cancer is associated with elevated androgen biosynthesis; therefore, inhibiting the activity of Cytochrome P450 17 (CYP17) may prevent progression of prostate cancer. In this study we identified, using in silico and experimental methods, two novel steroidal and non-steroidal lead compounds that inhibit the activity CYP17.
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    Does metformin prevent short term oxidant-induced DNA damage? In vitro study on lymphocytes from aged subjects
    (Walter de Gruyter, 2007) Kanigür Sultuybek, Gönül; Onaran, İlhan; Çurgunlu, Aslı; Tezcan, Vecdet; N/A; Özdaş, Şule Beyhan; Researcher; Graduate School of Sciences and Engineering; N/A
    Metformin(l-(diaminomethylidene)-3,3-dimethyl-guanidine), an anti-hyperglycemic agent, also has antioxidant effects. Although the origin is not clearly understood, the antioxidant activity of metformin might result from a direct effect on reactive oxygen species (ROS) or could have an indirect action on the superoxide anions produced by hyperglycemia. The ability of metformin to modulate DNA damage produced by oxidative stress is not known. For this reason, we examined the short term effect of metformin (50μM, 2 h) on the DNA damage of cumene hydroperoxide (CumOOH)-induced lymphocytes from aged and young control groups (n = 10 each). In this study, DNA damage elicited by CumOOH (1 mM) was detected with the Comet Assay and the ELISA technique. Our results showed a significant increase in apoptotic DNA fragmentation and DNA strand breaks (Comet assay tail factor %) that was detected before and after CumOOH induction in lymphocytes of healthy elderly subjects when compared with healthy young control.
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    Metformin does not prevent DNA damage in lymphocytes despite its antioxidant properties against cumene hydroperoxide-induced oxidative stress
    (Elsevier, 2006) Onaran, Ihan; Guven, Gulgun S.; Kanigur, Gonul; Vehid, Suphi; N/A; Özdaş, Şule Beyhan; Researcher; Graduate School of Sciences and Engineering; N/A
    Metformin (1-(diaminomethylidene)-3.3-dimethyl-guanidine), which is the most commonly prescribed oral antihyperglycaemic drug in the world, was reported to have several antioxidant properties such as the inhibition of advanced glycation end-products. In addition to its use in the treatment of diabetes, it has been suggested that metformin may be a promising anti-aging agent. The present work was aimed at assessing the possible protective effects of metformin against DNA-damage induction by oxidative stress in vitro. The effects of metformin were compared with those of N-acetylcysteine (NAC). For this purpose, peripheral blood lymphocytes from aged (n = 10) and young (n = 10) individuals were pre-incubated with various concentrations of metformin (10-50 mu M), followed by incubation with 15 mu M cumene hydroperoxide (CumOOH) for 48 h, under conditions of low oxidant level, which do not induce cell death. Protection against oxidative DNA damage was evaluated by use of the Comet assay and the cytokinesis-block micronucleus technique. Changes in the levels of malondialdehyde + 4-hydroxy-alkenals, an index of oxidative stress, were also measured in lymphocytes. At concentrations ranging from 10 mu M to 50 mu M. metformin did not protect the lymphocytes from DNA damage, while 50 VLM NAC possessed an effective protective effect against CumOOH-induced DNA damage. Furthermore, NAC, but not metformin. inhibited DNA fragmentation induced by CumOOH. In contrast to the lack of protection against oxidative damage in lymphocyte cultures. metformin significantly protected the cells from lipid peroxidation in both age groups, although not as effective as NAC in preventing the peroxidative damage at the highest doses. Within the limitations of this study, the results indicate that pharmacological concentrations of metformin are unable to protect against DNA damage induced by a pro-oxidant stimulus in cultured human lymphocytes, despite its antioxidant properties.
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    Transcriptional regulation of the ADP-glucose pyrophosphorylase isoforms in the leaf and the stem under long and short photoperiod in lentil
    (Elsevier Ireland Ltd, 2013) Morgil, Hande; Tulum, Işıl; Cevahir, Gül; N/A; Department of Molecular Biology and Genetics; Department of Chemical and Biological Engineering; Seferoğlu, Ayşe Bengisu; Barış, İbrahim; Özdaş, Şule Beyhan; Kavaklı, İbrahim Halil; PhD Student; Teaching Faculty; Researcher; Faculty Member; Department of Molecular Biology and Genetics; Department of Chemical and Biological Engineering; Graduate School of Sciences and Engineering; College of Sciences; Graduate School of Sciences and Engineering; College of Engineering; N/A; 111629; 40319
    ADP-glucose pyrophosphorylase (AGPase) is a key enzyme in plant starch biosynthesis. It contains large (LS) and small (SS) subunits encoded by two different genes. In this study, we explored the transcriptional regulation of both the LS and SS subunits of AGPase in stem and leaf under different photoperiods length in lentil. To this end, we first isolated and characterized different isoforms of the LS and SS of lentil AGPase and then we performed quantitative real time PCR (qPCR) to see the effect of photoperiod length on the transcription of the AGPase isforms under the different photoperiod regimes in lentil. Analysis of the qPCR results revealed that the transcription of different isoforms of the LSs and the SSs of lentil AGPase are differentially regulated when photoperiod shifted from long-day to short-day in stem and leaves. While transcript levels of LS1 and SS2 in leaf significantly decreased, overall transcript levels of SS1 increased in short-day regime. Our results indicated that day length affects the transcription of lentil AGPase isoforms differentially in stems and leaves most likely to supply carbon from the stem to other tissues to regulate carbon metabolism under short-day conditions.
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    The increased genotoxicity following inhibition of the glutathione S-conjugate transport in K562 cells
    (Blackwell Publishing, 2006) Onaran, I.; Kanigur, G; N/A; Özdaş, Şule Beyhan; Researcher; Graduate School of Sciences and Engineering; N/A
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