Researcher:
Süer, Hande

Job Title

PhD Student

First Name

Hande

Last Name

Süer

Name

Name Variants

Süer, Hande

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Search Results

Now showing 1 - 5 of 5
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    PublicationOpen Access
    Development of a low allergenic product for patients with milk allergy and assessment of its specific IgE reactivity
    (Wiley, 2024) Nemutlu, Emirhan; Kocak, Engin; Yazıcı, Duygu; Süer, Hande; Bülbüloğlu, Cemre Naz; Güzar, Elif; Büyüktiryaki, Ayşe Betül; Saçkesen, Cansın; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; School of Medicine;  
    Background: Milk oral immunotherapy is the riskiest and most unpredictable form of oral immunotherapy. We aimed to produce a low allergenic product than conventional once baked-cake/muffin, to develop indirect in-house ELISA to check the tolerance status with milk products and evaluate IgE reactivity of patients' sera via western blotting (WB) and indirect in-house ELISA. Method: A low allergenic product named biscotti-twice baked-cake was developed, and the total protein concentration was determined. The protein content was studied by SDS-PAGE and proteomics. Milk-specific IgE (sIgE) binding assays were performed by WB and indirect in-house ELISA by using patients' sera. Results: Casein band intensity was observed to be lower in the biscotti-twice baked-cake than in the once baked-cake (p = .014). Proteomics analysis and alpha S1-casein measurement showed that the lowest intensity of casein was found in biscotti. The low binding capacity of milk sIgE to biscotti compared with once baked-cake was shown by WB (p = .0012) and by indirect in-house ELISA (p = .0001). In the ROC analysis, the area under the curve (AUC) of the in-house ELISA IgE was comparable with Uni-CAP milk and casein sIgE. The AUC of the in-house ELISA IgE for cake (0.96) and biscotti (1) was slightly better than Uni-CAP milk sIgE (0.94; 0.97) and casein sIgE (0.96; 0.97), respectively. Conclusion: The low allergenicity of the newly developed low allergenic product "biscotti-twice baked-cake" has been demonstrated by in vitro experiments. Biscotti could be a safe treatment option than once baked-cake/muffin in patients who are reactive to once baked-milk products.
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    Publication
    Investigation of the effects of tumor necrosis factor-alpha, H2o2, ethanol and butyrate on intestinal epithelial cell permeability
    (Wiley, 2019) Yazıcı, Duygu; Süer, Hande; Yılmaz, Özlem; Karahüseyinoğlu, Serçin; Saçkesen, Cansın; PhD Student; PhD Student; Master Student; Faculty Member; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; Graduate School of Health Sciences; Graduate School of Sciences and Engineering; School of Medicine; School of Medicine; N/A; N/A; N/A; 110772; 182537
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    Publication
    Predicting micrometastasis in mediastinal lymph nodes in patients with non-small cell lung cancer
    (Elsevier Science Inc, 2019) Kapdagli, M.; N/A; N/A; N/A; N/A; Erus, Suat; Süer, Hande; Tanju, Serhan; Dilege, Şükrü; Faculty Member; PhD Student; Faculty Member; Faculty Member; School of Medicine; Graduate School of Health Sciences; School of Medicine; School of Medicine; Koç University Hospital; 175565; N/A; 214690; 122573
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    Publication
    Investigation of heat and matrix effect on milk proteins' allergenicity and the development of hypoallergenic food products
    (Wiley, 2018) N/A; N/A; Department of Chemical and Biological Engineering; N/A; Department of Chemical and Biological Engineering; Süer, Hande; Yazıcı, Duygu; Erman, Burak; Saçkesen, Cansın; PhD Student; PhD Student; Faculty Member; Faculty Member; Graduate School of Health Sciences; Graduate School of Health Sciences; College of Engineering; School of Medicine; N/A; N/A; 179997; 182537
    Background: Cow's milk allergy is one of the most common foodallergies during childhood. It has been shown that high heat can reduce some milk proteins’allergenicity (ß‐lactoglobulin). In this pro-ject we aimed to investigate the effect of heat and matrix on differ-ent milk protein fractions through Maillard reaction and eventuallydevelop hypoallergenic food products that have milk protein withlow reaction risk.Method: Milk cake matrix is prepared in different flour/sugar (F/S)ratio (2F/1S, 1F/1S, 0.5F/1S) and baked 30 minutes at 180°C. Pro-teins that cake contains are separated using SDS PAGE and stainedwith coomassie blue to check total protein. In parallel specific pro-teins are detected by western blotting using pooled sera frompatients with milk specific IgE>60kU/L for incubation. Results: In normal milk cake recipe (2F/1S)ß‐lactoglobulin bandsare disappeared but casein bands did not differ in size. In order toinvestigate the matrix effect F/S ratio is changed and it is found thatwhen this ratio decreases, with the affect of heat and maillard reac-tion, milk casein bands’intensities also decrease in SDS gel coomas-sie staining. In western blot experiments it is also shown that milkspecific IgE bound weakly to casein bands in low F/S ratio cake(0.5F/1S) whereas in cakes that have high F/S (2F/1S) ratio it boundsignificantly higher. Conclusion: Heat and matrix effect cause disruptions in milk caseinandß‐lactoglobulin proteins’structure and lower the milk specificIgE bindings to milk proteins in low F/S ratio cake through Maillard reaction.
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    PublicationOpen Access
    Liposomes as potential nanocarriers for theranostic applications in chronic inflammatory lung diseases
    (Medknow Publications, 2017) Süer, Hande; Bayram, Hasan; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; Graduate School of Health Sciences; N/A; 4890
    Chronic obstructive pulmonary disease and asthma affect millions of people worldwide. Conventional treatments are not sufficient at preventing the progression of these diseases. The treatments are unspecific, and when administered systematically, and in high doses, they have adverse health effects. Further, the current diagnostic methods are poor and they are not capable of identifying the accurate state of the disease. Nanoparticle-based theranostics (NBTs) are well-established systems that simultaneously provide treatment and diagnostics. Various nanocarriers can be used in NBT and are designed according to the need. In this review, liposome-based theranostic systems are evaluated and possible modifications that could provide better drug delivery and accumulation and diagnosis are exemplified. These systems can be applied to conventional therapeutics to avoid side effects and perhaps overcome the challenges that interfere with their cellular accumulation.