Researcher:
Kısakürek, Zeynep Büşra

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Undergraduate Student

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Zeynep Büşra

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Kısakürek

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Kısakürek, Zeynep Büşra

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2021 - 20228

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Researcher Of Publications: search.filters.isAuthorOfPublication.0d6c7948-ea30-47b6-a5d4-9c40ceb78d83

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Now showing 1 - 8 of 8
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    The impact of dietary salicylate on asthma control in acetylsalicylic acid-exacerbated respiratory disease
    (Wiley, 2021) Gumusburun, R; Göksel, O.; N/A; İncir, Sevil; Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Öztürk, Ayşe Bilge; Saçkesen, Cansın; Doctor; Other; Undergraduate Student; Faculty Member; Faculty Member; N/A; School of Medicine; School of Medicine; School of Medicine; School of Medicine; Koç University Hospital; N/A; N/A; N/A; N/A; N/A; N/A; N/A; 147629; 182537
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    Quality of management in drug-induced anaphylaxis: an analysis of yearlong data in a tertiary care setting
    (Wiley, 2021) N/A; N/A; N/A; N/A; Kısakürek, Zeynep Büşra; Sönmez, Sadi Can; Öztürk, Ayşe Bilge; Undergraduate Student; Other; Faculty Member; School of Medicine; Koç University Hospital; School of Medicine; N/A; N/A; 147629
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    Calcium/protein kinase C signaling mechanisms in shear-induced mechanical responses of red blood cells
    (Academic Press Inc Elsevier Science, 2021) N/A; N/A; N/A; N/A; N/A; N/A; Uğurel, Elif; Kısakürek, Zeynep Büşra; Aksu, Yasemin; Göksel, Evrim; Çilek, Neslihan; Yalçın, Özlem; Researcher; Undergraduate Student; Undergraduate Student; PhD Student; PhD Student; Faculty Member; School of Medicine; School of Medicine; School of Medicine; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; N/A; N/A; N/A; N/A; N/A; 218440
    Red blood cell (RBC) deformability has vital importance for microcirculation in the body, as RBCs travel in narrow capillaries under shear stress. Deformability can be defined as a remarkable cell ability to change shape in response to an external force which allows the cell to pass through the narrowest blood capillaries. Previous studies showed that RBC deformability could be regulated by Ca2+/protein kinase C (PKC) signaling mechanisms due to the phosphorylative changes in RBC membrane proteins by kinases and phosphatases. We investigated the roles of Ca2+/PKC signaling pathway on RBC mechanical responses and impaired RBC deformability under continuous shear stress (SS). A protein kinase C inhibitor Chelerythrine, a tyrosine phosphatase inhibitor Calpeptin, and a calcium channel blocker Verapamil were applied into human blood samples in 1 micromolar concentration. Samples with drugs were treated with or without 3 mM Ca2+. A shear stress at 5 Pa level was applied to each sample continuously for 300 s. RBC deformability was measured by a laser-assisted optical rotational cell analyzer (LORRCA) and was calculated as the change in elongation index (EI) of RBC upon a range of shear stress (SS, 0.3-50 Pa). RBC mechanical stress responses were evaluated before and after continuous SS through the parameterization of EI-SS curves. The drug administrations did not produce any significant alterations in RBC mechanical responses when they were applied alone. However, the application of the drugs together with Ca2+ substantially increased RBC deformability compared to calcium alone. Verapamil significantly improved Ca2+-induced impairments of deformability both before and after 5 Pa SS exposure (p < 0.0001). Calpeptin and Chelerythrine significantly ameliorated impaired deformability only after continuous SS (p < 0.05). Shear-induced improvements of deformability were conserved by the drug administrations although shear-induced deformability was impaired when the drugs were applied with calcium. The blocking of Ca2+ channel by Verapamil improved impaired RBC mechanical responses independent of the SS effect. The inhibition of tyrosine phosphatase and protein kinase C by Calpeptin and Chelerythrine, respectively, exhibited ameliorating effects on calcium-impaired deformability with the contribution of shear stress. The modulation of Ca2+/ PKC signaling pathway could regulate the mechanical stress responses of RBCs when cells are under continuous SS exposure. Shear-induced improvements in the mechanical properties of RBCs by this signaling mechanism could facilitate RBC flow in the microcirculation of pathophysiological disorders, wherein Ca2+ homeostasis is disturbed and RBC deformability is reduced.
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    The cellular degradative process in epithelial cells of lens capsule with exfoliation syndrome: A preliminary report
    (Assoc Research Vision Ophthalmology Inc, 2021) N/A; N/A; N/A; N/A; N/A; N/A; Karslıoğlu, Melisa Zişan; Aydemir, Dilara; Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Şahin, Afsun; Doctor; PhD Student; Other; Undergraduate Student; Faculty Member; Koç University Hospital; Graduate School of Health Sciences; Koç University Hospital; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; 171267
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    The immunohistochemical analysis of cellular auto-degradative functions in epithelial cells of the anterior capsule of lens in patients with pseudo-exfoliation syndrome: a preliminary report
    (Assoc Research Vision Ophthalmology Inc, 2022) N/A; N/A; N/A; N/A; N/A; N/A; Sönmez, Sadi Can; Karslıoğlu, Melisa Zişan; Aydemir, Dilara; Gözel, Merve; Kısakürek, Zeynep Büşra; Şahin, Afsun; Undergraduate Student; Doctor; PhD Student; PhD Student; Undergraduate Student; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; N/A; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; N/A; 171267
    Purpose : Pseudo-exfoliation syndrome (PXF) is a systemic disease in which fibrillary proteinaceous material is deposited within the tissues such as the eye, which is thought to arise from dysfunctional matrix turnover and intracellular auto-degradation pathways. In this case-control study setting, we aim to evaluate whether defects in mitophagy specifically have a role in PXF etiology and prognosis. Methods : The anterior lens capsules obtained in microincisional cataract surgery were collected both from patients with PXF (n=6) and age-matched healthy controls (n=10). The samples were embedded in paraffin blocks and sliced into 0.4 μm slides for analysis. The ABC® IHC detection kit was used to stain the samples for PTEN-induced kinase 1 (PINK1), Parkin and LC3B. Stained images were captured with 20x magnification under confocal microscopy. The expression intensity was measured using color deconvolution plug-in and integrated density value was calculated on Fiji® (U. S. National Institutes of Health, Bethesda, MD). The Mann-Whitney U test was used for statistical analysis and the results were shown on Graphpad Prism (Graphpad, San Diego, CA). Results : The staining intensity of PINK1 and PARKIN were higher in the epithelia of anterior lens capsule in patients with PXF compared to the control samples, which was only statistically significant for PINK1. (p=0.0002, p=0.999 respectively) LC3B intensity was also found to be increased in PXF patients with statistical significance (p=0.0006). (Images 1 and 2). Conclusions : This increase in the markers of mitophagy, which is the targeted elimination of dysfunctional mitochondria, may be an important indicator of increased oxidative stress in PXF. It can therefore signal an extensive mitochondrial impairment and higher generation of reactive oxygen species, potentially overburdening the cellular clearance and enhancing faulty protein release. Certainly, further in vitro studies with a larger sample number are needed to compare the actual stress responses dynamically in PXF.
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    PublicationOpen Access
    Larynx angioedema as a signal in chronic lymphocytic leukemia: a case based guide for acquired angioedema in the setting of lymphoproliferative disorders
    (Buluş Design, 2021) Öztürk, Erman; Öztürk, Ayşe Bilge; Kısakürek, Zeynep Büşra; Sönmez, Sadi Can; Osmanbaşoğlu, Emre; Faculty Member; School of Medicine; Koç University Hospital; 147629; N/A; N/A; N/A
    Patients with angioedema can present to the internal medicine, emergency medicine, dermatology, or ear nose throat clinics. Physicians may need to assess the patients whose angioedema is unresponsive to antihistamines systematically in collaboration with other subspecialties including hematology, rheumatology, allergy, and immunology. We aimed to provide a concise review of the diagnosis and multi-disciplinary management of acquired angioedema through a case presentation. A 61-year-old woman presented with recurrent angioedema of 4 episodes within one year. She was evaluated by various disciplines such as dermatology and emergency medicine. Antihistamines and steroids were not effective. The complete blood count (CBC) results indicated lymphocytosis (lymphocyte count=9100 k/mu L) and further evaluation of the lymphocytosis with flow cytometry immunophenotyping confirmed a diagnosis of chronic lymphocytic leukemia. Since the acquired angioedema diagnosis was confirmed with low C4, C1q, and C1 esterase inhibitor levels, Rituximab 375 mg/m(2) was administered once a week for 4 weeks. The frequency of attacks decreased after rituximab therapy and none of them were life-threatening. In conclusion, when the effective treatment is initiated for the primary diagnosis in acquired angioedema, the numerous emergency department visits, hospitalizations, and the mortality due to life-threatening angioedema episodes can be avoided.
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    PublicationOpen Access
    Developing a measure to quantify ocular pain postoperatively: the adaptation of the Ocular Pain Assessment Survey
    (Hindawi, 2022) Öztürkmen, Cem; Kurt, Rengin Aslıhan; Hamrah, Pedram; Şahin, Afsun; Taş, Ayşe Yıldız; Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Deniz, Gülsüm; Baygül, Arzu Eden; Kesim, Cem; Karslıoğlu, Melisa Zişan; Faculty Member; Faculty Member; Teaching Faculty; Doctor; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; Koç University Hospital; 171267; 200905; N/A; N/A; N/A; 272290; N/A; N/A
    Purpose: since quantification and communication of ocular pain is important for a healthier patient follow-up and postoperative guidance, reliable measures like the Ophthalmic Pain Assessment Survey (OPAS) are needed to assess the outcome and management of different operations. To address that need, we carried out the adaptation of OPAS into Turkish to reach different age groups and backgrounds, widening the use of OPAS on patients who underwent an ophthalmic operation. Methods: we used back-translation method and achieved cultural adaptation through content validity scoring by 5 independent ophthalmologists. The survey is then administered three times: preoperatively, postoperatively within 24 hours, and finally a week later in the follow-up visit. Validity is measured in comparison to Visual Analog Scale using Spearman's correlation coefficient and reliability is measured using Cronbach's alpha. Factor analysis is performed by principal component analysis and rotation is performed using Varimax method when necessary. Results: we reached a total of 132 patients with a mean age of 64.2 years. Most of them underwent phacoemulsification (n = 83), followed by PRK (n = 37). Overall, the T-OPAS demonstrated good reliability (mean C. alpha: 0.830) and its correlation with the VAS was especially high (S. coeff. >0.5) in the first three sections in all three surveys. Factor analysis yielded 5 subscales, allowing us to shape the final form of T-OPAS. Conclusion: through this adaptation of OPAS into a foreign language, we present a reliable and valid tool for postoperative pain quantification, allowing objective measurement of pain in different populations such as the elderly.
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    PublicationOpen Access
    Covid-19, severe asthma and omalizumab therapy: a case-based inquiry into associations, management, and the possibility of a better outcome
    (Aves, 2021) Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Öztürk, Ayşe Bilge; Tekin, Süda; Faculty Member; School of Medicine; N/A; N/A; 147629; 42146
    Recent developments in research have put forward claims on the protective effect of allergic diseases especially allergic airway inflammation including allergic asthma—against the COVID-19 pandemic. This was first suggested by the underrepresentation of asthma patients in some cohorts and was later investigated by angiotensin-converting enzyme II (ACEII) receptor expression studies. Controversial data on this matter persists and continue to be a challenge for clinicians when it comes to managing allergic diseases during the pandemic. We present in this study a thorough review of related findings so far and our own experience with 2 severe asthma patients who presented with atypical symptoms. Interestingly, both patients were administered anti-IgE therapy prior to the initial positive polymerase chain reaction (PCR) results for COVID-19 and showed no symptoms of severe respiratory disease during the infection unlike what we know from other viral respiratory illnesses. Stemming from that, we wanted to combine the perspectives of allergists and infectious disease specialists to address certain concerns in the management of allergic diseases, such as biologicals, in the light of current guidelines. We have also pointed out certain gaps in clinical and molecular level research, such as the lack of phenotypical subgroup analysis among ill asthma patients and the lack of data concerning the molecular effects of biologicals on viral infection.