Researcher: Manioğlu, Selen
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Manioğlu, Selen
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Publication Metadata only Photochromic FRET sensors to monitor heme protein dynamics(Cell Press, 2014) Department of Chemistry; N/A; Department of Chemistry; Bayraktar, Halil; Manioğlu, Selen; Faculty Member; Master Student; College of Sciences; Graduate School of Sciences and Engineering; 201764; N/AN/APublication Metadata only Quantitative FRET microscopy to study redox protein maturation and interactions in living cells(Springer, 2015) N/A; N/A; Department of Chemistry; Department of Chemistry; Manioğlu, Selen; Bayraktar, Halil; Master Student; Faculty Member; Graduate School of Sciences and Engineering; College of Sciences; N/A; 201764N/APublication Metadata only Monitoring redox activity of heme proteins by photochromic fluorescence resonance energy transfer(Springer, 2013) Department of Chemistry; N/A; Department of Chemistry; Bayraktar, Halil; Manioğlu, Selen; Faculty Member; Master Student; College of Sciences; Graduate School of Sciences and Engineering; 201764; N/AN/APublication Metadata only Dynamic estimation of FRET correction factors to study redox protein interactions(Cell Press, 2017) Department of Chemistry; N/A; Department of Chemistry; Bayraktar, Halil; Manioğlu, Selen; Faculty Member; Master Student; College of Sciences; Graduate School of Sciences and Engineering; 201764; N/AN/APublication Open Access In vitro and in vivo biolasing of fluorescent proteins suspended in liquid microdroplet cavities(Royal Society of Chemistry (RSC), 2014) Jonas, Alexandr; Anand, Suman; McGloin, David; Department of Physics; Department of Chemistry; Department of Physics; Department of Chemistry; Bayraktar, Halil; Kiraz, Alper; Aas, Mehdi; Karadağ, Yasin; Manioğlu, Selen; Faculty Member; Faculty Member; PhD Student; College of Sciences; N/A; 22542; N/A; N/A; N/AFluorescent proteins are indispensable for selective, quantitative visualization of localization, dynamics, and interactions of key molecular constituents of live cells. Incorporation of fluorescent proteins into an optical cavity can lead to a significant increase in fluorescence signal levels due to stimulated emission and light amplification in the cavity, forming a laser with biological gain medium. Utilization of lasing emission from fluorescent biological molecules can then greatly enhance the performance of fluorescence-based biosensors benefiting from the high sensitivity of non-linear lasing processes to small perturbations in the cavity and the gain medium. Here we study optofluidic biolasers that exploit active liquid optical resonators formed by surface-supported aqueous microdroplets containing purified yellow fluorescent protein or a suspension of live E. coli bacterial cells expressing the fluorescent protein. We first demonstrate lasing in fluorescent protein solutions at concentrations as tow as 49 mu M. Subsequently, we show that a single fluorescent bacterial cell of micrometre size confined in a droplet-based cavity can serve as a laser gain medium. Aqueous droplet microcavities allow the maintenance of the bacterial cells under conditions compatible with unimpeded growth. Therefore, our results also suggest a direct route to microscopic sources of laser light with self-regenerating gain media.Publication Open Access Direct conversion of cytochrome c spectral shifts to fluorescence using photochromic FRET(Royal Society of Chemistry (RSC), 2014) Department of Physics; Department of Chemistry; Department of Chemical and Biological Engineering; Department of Physics; Department of Chemistry; Department of Chemical and Biological Engineering; Manioğlu, Selen; Atış, Müge; Aas, Mehdi; Kiraz, Alper; Bayraktar, Halil; PhD Student; Faculty Member; Faculty Member; College of Sciences; N/A; N/A; N/A; 22542; N/APhotochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c.