Researcher:
Harmanda, Büşra

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Büşra

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Harmanda

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Harmanda, Büşra

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2015 - 20162

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Researcher Of Publications: search.filters.isAuthorOfPublication.39b2e455-0baf-4bef-9901-96206947b476

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    Phosphoproteomic analysis of aurora kinase inhibition in monopolar cytokinesis
    (Amer Chemical Soc, 2015) Giese, Sven H.; Hu, Chi-Kuo; Renard, Bernhard Y.; N/A; N/A; N/A; N/A; Department of Molecular Biology and Genetics; Köken, Ayşe Nur Polat; Karayel, Özge; Harmanda, Büşra; Şanal, Erdem; Master Student; Master Student; Researcher; Master Student; Faculty Member; Department of Molecular Biology and Genetics; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; 239008; N/A; N/A; N/A; 105301
    Cytokinesis is the last step of the cell cycle that requires coordinated activities of the microtubule c-ytoskeleton, actin cytoskeleton, and membrane compartments. Aurora B kinase is one of the master regulatory kinases that orchestrate multiple events during cytokinesis. To reveal targets of the Aurora B kinase, we combined quantitative mass spectrometry with chemical genetics. Using the quantitative proteomic approach, SILAC (stable isotope labeling with amino acids in cell culture), we analyzed the phosphoproteome of monopolar cytokinesis upon VX680- or AZD1152-mediated aurora kinase inhibition. In total, our analysis quantified over 20 000 phosphopeptides in response to the Aurora-B kinase inhibition; 246 unique phosphopeptides were significantly down-regulated and 74 were up-regulated. Our data provide a broad analysis of downstream effectors of Aurora kinase and offer insights into how Aurora kinase regulates cytokinesis.
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    A small molecule identified through an in silico screen inhibits Aurora B–INCENP interaction
    (Wiley, 2016) N/A; N/A; N/A; Department of Chemical and Biological Engineering; Department of Molecular Biology and Genetics; Ünsal, Esra; Harmanda, Büşra; Erman, Burak; Master Student; N/A; PhD Student; Faculty Member; Faculty Member; Department of Chemical and Biological Engineering; Department of Molecular Biology and Genetics; Graduate School of Sciences and Engineering; N/A; Graduate School of Sciences and Engineering; College of Engineering; College of Sciences; N/A; N/A; N/A; 179997; 105301
    Aurora B is a serine/threonine kinase that has a central role in the regulation of mitosis. The observation of Aurora B overexpression in cancer makes it a promising target to develop antitumoral inhibitors. We describe a new potential inhibitor that exclusively targets the interaction site of Aurora B and its activator INCENP. We performed a structure-based virtual screening and determined five potential candidates of 200000 compounds, which selectively bind to the Aurora B