Researcher:
Aydemir, Dilara

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PhD Student

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Dilara

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Aydemir

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Aydemir, Dilara

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2020 - 20225

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Researcher Of Publications: search.filters.isAuthorOfPublication.3b9e7211-3af7-49ac-b9c2-7e7136d6e876

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    Investigation of mitophagy biomarkers in corneal epithelium of keratoconus patients
    (Taylor and Francis Inc, 2022) Gumus, Koray; Sarac, Ozge Ilhan; Cagil, Nurullah; N/A; N/A; N/A; N/A; N/A; N/A; Yıldız, Erdost; Aydemir, Dilara; Zibandeh, Noushin; Ünlü, Eda Kuşan; Karslıoğlu, Melisa Zişan; Şahin, Afsun; PhD Student; PhD Student; Researcher; Researcher; Doctor; Faculty Member; Graduate School of Health Sciences; Graduate School of Health Sciences; N/A; Graduate School of Health Sciences; N/A; School of Medicine; N/A; N/A; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); N/A; Koç University Hospital; N/A; N/A; N/A; N/A; N/A; N/A; 171267
    Purpose: The pathological mechanisms of keratoconus (KC) have not been elucidated yet. Mitophagy is an important mechanism that eliminates damaged mitochondria under oxidative stress, and it could be one of the leading pathological causes of KC. This study aimed to find out the role of mitophagy in the keratoconic corneal epithelium. Methods: The corneal epithelia were collected from the 103 progressive KC patients and the 46 control subjects. The real-time quantitative PCR was performed for PTEN-putative kinase-1 (PINK1), PARKIN, p62, and BNIP3 gene expressions in 31 KC and 9 control subjects. Western blot analyses were performed to investigate the protein expressions of PINK1, PARKIN, LC3B, ATG5, and BECLIN in the remaining 109 corneal epithelium samples from 72 patients and 37 control subjects. Results: mRNA and protein expressions of PINK1 decreased significantly in the corneal epithelium of KC patients compared to the control subjects. No significant change was found in mRNA levels of PARKIN, p62, and BNIP3 in KC patients. The protein expression of PARKIN, LC3B, ATG5, and Beclin did not significantly differ between KC patients and control subjects. Gene expression levels of mitophagy biomarkers were not affected by the KC grade. Conclusions: PINK1/PARKIN-dependent mitophagy is affected in the keratoconic corneal epithelium. We found significant decreases in both mRNA and protein expressions of PINK1 in the keratoconic corneal epithelium. However, we did not observe any other significant change in mitophagy markers. Mitochondrial stress-related mitophagy pathways could be interrupted by the decreased levels of PINK1 in the keratoconic corneal epithelium, but solely PINK1 dysregulation is not likely to induce KC pathogenesis.
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    The cellular degradative process in epithelial cells of lens capsule with exfoliation syndrome: A preliminary report
    (Assoc Research Vision Ophthalmology Inc, 2021) N/A; N/A; N/A; N/A; N/A; N/A; Karslıoğlu, Melisa Zişan; Aydemir, Dilara; Sönmez, Sadi Can; Kısakürek, Zeynep Büşra; Şahin, Afsun; Doctor; PhD Student; Other; Undergraduate Student; Faculty Member; Koç University Hospital; Graduate School of Health Sciences; Koç University Hospital; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; 171267
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    Communications between adipose-derived mesenchymal stem cells (AdMSCs) and retinal pigment epithelial cell line (RPE-1) in different stress environments via tunneling nanotubes
    (Association for Research in Vision and Ophthalmology (ARVO), 2022) N/A; N/A; N/A; N/A; N/A; N/A; Gözel, Merve; Aydemir, Dilara; Şenköylü, Karya; Kabadayı, Berk; Şahin, Afsun; Hasanreisoğlu, Murat; PhD Student; PhD Student; Undergraduate Student; Undergraduate Student; Faculty Member; PhD Student; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM; N/A; N/A; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; School of Medicine; Graduate School of Sciences and Engineering; N/A; N/A; N/A; /N/A; 171267; N/A
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    Does brimonidine tartarate have any effect on viability and/or proliferation of limbal mesenchymal limbal stem cells?
    (Assoc Research Vision Ophthalmology Inc, 2020) N/A; N/A; N/A; N/A; N/A; N/A; Karslıoğlu, Melisa Zişan; Zibandeh, Noushin; Aydemir, Dilara; Ünlü, Eda Kuşan; Kesim, Cem; Taş, Ayşe Yıldız; Şahin, Afsun; Doctor; Researcher; PhD Student; Researcher; Researcher; Doctor; Faculty Member; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Koç University Hospital; N/A; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; 387367; 200905; 171267
    Purpose : Mesenchymal stem cell transplantation is still one of the hot topics within translational medicine. The limbus, rim of the corneoscleral junction of human eye, is highly rich in terms of stem cells. Limbal mesenchymal stem cells (LMSCs) have been used in many ocular diseases. Brimonidine tartrate (BT) is selective alpha-2 adrenergic agonist that its neuroprotective effect had been proven. BT drops are preferred in glaucoma patients especially for neuroprotection. Our purpose is to evaluate the effect of BT on viability and/or proliferation of LMSCs in vitro. Methods : LMSCs were isolated from corneoscleral rim. The cells in third passage were characterized and differentiated. For MTT assay, cells were seeded in the density of 3x103 in 96 well plate and pretreatment with BT in 0.1, 1, 5,10 and 200 μM concentrations. After 48 hours, MTT solution was added to each wells and plate was read in 570nm absorbance. For scratch assay, cells were seeded in the density of 6x104 in 12 well plate and pretreatment with BT in pervious mentioned concentrations. For Annexin V/PI cell viability test, cells were seeded in the density of 2x105 in 6 well plate and pretreatment with BT in pervious mentioned concentrations. After 48 hours, cells were washed and stained with Annexin V The cells were analysed by flowcytometry. Results : Our experiments showed that pre-treated LMSCs with 10 μM BT increased cell viability significantly compared to untreated cells in MTT assay (p<0.05). In scratch assay pre-treated LMSCs with 5 μM and 10 μM BT displayed statistically significant rapid migration at 20th hours regarding to other untreated cells (p<0.05). According to cell viability results, pre-treated LMSCs with 10 μM BT decreased Annexin V expression compared to untreated group. And it is statistically significant (p<0.05). Conclusions : Since limbal mesenchymal stem cells and optic nerve share the same embryological origin (ectodermal), it is not surp rising that in our study BT enhanced the viability of limbal mesenchymal stem cells and improved proliferation. From this point of view, we hypothesized that intravitreally injection of brimonidine tartrate loaded limbal mesenchymal stem cells may not only protect axon loss, but also induce axon regeneration in traumatic optic nerve injuries. This preliminary study supported our hypothesis and we are in the process of planning future studies about this topic.
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    The immunohistochemical analysis of cellular auto-degradative functions in epithelial cells of the anterior capsule of lens in patients with pseudo-exfoliation syndrome: a preliminary report
    (Assoc Research Vision Ophthalmology Inc, 2022) N/A; N/A; N/A; N/A; N/A; N/A; Sönmez, Sadi Can; Karslıoğlu, Melisa Zişan; Aydemir, Dilara; Gözel, Merve; Kısakürek, Zeynep Büşra; Şahin, Afsun; Undergraduate Student; Doctor; PhD Student; PhD Student; Undergraduate Student; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; N/A; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; N/A; N/A; N/A; N/A; N/A; 171267
    Purpose : Pseudo-exfoliation syndrome (PXF) is a systemic disease in which fibrillary proteinaceous material is deposited within the tissues such as the eye, which is thought to arise from dysfunctional matrix turnover and intracellular auto-degradation pathways. In this case-control study setting, we aim to evaluate whether defects in mitophagy specifically have a role in PXF etiology and prognosis. Methods : The anterior lens capsules obtained in microincisional cataract surgery were collected both from patients with PXF (n=6) and age-matched healthy controls (n=10). The samples were embedded in paraffin blocks and sliced into 0.4 μm slides for analysis. The ABC® IHC detection kit was used to stain the samples for PTEN-induced kinase 1 (PINK1), Parkin and LC3B. Stained images were captured with 20x magnification under confocal microscopy. The expression intensity was measured using color deconvolution plug-in and integrated density value was calculated on Fiji® (U. S. National Institutes of Health, Bethesda, MD). The Mann-Whitney U test was used for statistical analysis and the results were shown on Graphpad Prism (Graphpad, San Diego, CA). Results : The staining intensity of PINK1 and PARKIN were higher in the epithelia of anterior lens capsule in patients with PXF compared to the control samples, which was only statistically significant for PINK1. (p=0.0002, p=0.999 respectively) LC3B intensity was also found to be increased in PXF patients with statistical significance (p=0.0006). (Images 1 and 2). Conclusions : This increase in the markers of mitophagy, which is the targeted elimination of dysfunctional mitochondria, may be an important indicator of increased oxidative stress in PXF. It can therefore signal an extensive mitochondrial impairment and higher generation of reactive oxygen species, potentially overburdening the cellular clearance and enhancing faulty protein release. Certainly, further in vitro studies with a larger sample number are needed to compare the actual stress responses dynamically in PXF.