Researcher: Karahüseyinoğlu, Serçin
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Karahüseyinoğlu, Serçin
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Publication Metadata only Splicing variants of versican in CD133+/CD44+ prostate cancer stem cells(Elsevier GMBH, 2024) Ayla, Sule; Karakoc, Emre; Byrne, Yasemin Yozgat; Parlayan, Cuneyd; Keskin, Ilknur; Taskiran, Aysegul; Oktem, Gulperi; Karahüseyinoğlu, Serçin; ; School of Medicine;A cancer mass is composed of a heterogeneous group of cells, a small part of which constitutes the cancer stem cells since they are less differentiated and have a high capacity to develop cancer. Versican is an extracellular matrix protein located in many human tissues. The mRNA of versican has been shown to have "splicing patterns" as detected by RT-PCR, northern blot analysis, and cDNA sequencing. Based on this knowledge this study aims to reveal the splice variants of versican molecules, which are thought to be involved in the pathogenesis of the DU145 human prostatic carcinoma cell line and prostatic cancer stem cells isolated from this cell line. In this study, RWPE-1 normal prostatic and DU-145 human prostate cancer cell lines have been used. Prostatic cancer stem cells and the remaining group of non-prostatic-cancer stem cells (bulk population) were isolated according to their CD133+/CD44+. RNA was isolated in all groups, and sequence analysis was accomplished for splicing variants by Illumina NextSeq 500 sequencing system. The results were analyzed by bioinformatic evaluation. As five isoforms of the versican gene in the differential transcript expression are analyzed, it was observed that a significant change was only found in the isoforms Versican 0 and Versican 1. In this study, we explored the function of this molecule which we think to be effective in cancer progression, and suggested that more valuable results can be obtained after the accomplishment of in vivo experiments.Publication Metadata only The Impact of application of CRISPR /dCas9 systems for increasing the expression of FSH receptor in human granulosa cells(Oxford University Press, 2023) Güller, A. S.; Kayabölen, G. N. Şahin; Comar, M. Y.; Sivaslıoğlu, A.; Söyler, Gizem; Yılmaz, İpek; Karahüseyinoğlu, Serçin; ; Graduate School of Health Sciences; School of Medicine;N/APublication Metadata only The discriminative Crispr/Cas 9 basedgenome edition of endometrial cells and blastocysts can reveal genes newto implantation process in mouse(Elsevier Science Inc, 2023) Keskin, Ece; Karahüseyinoğlu, Serçin; Söyler, Gizem; Şahin, Gizem Nur; Yılmaz, İpek; Çomar, Mehmet Yunus; Ayhan, Ceyda Açılan; Taşkın, Ali Cihan; Öktem, Özgür; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; Graduate School of Health SciencesPublication Metadata only Global gene expression analysis of blastocyst implantation on 2D and 3D implantation models: towards a comprehensive and inclusive research(Oxford Univ Press, 2018) Sari, K.; Yucel, D.; N/A; Karahüseyinoğlu, Serçin; Faculty Member; School of Medicine; School of Medicine; 110772N/APublication Metadata only Wound healing effects of methanol extract of Laurocerasusofficinalis roem(Taylor & Francis, 2019) Ayla, S.; Okur, M. E.; Gunal, M. Y.; Ozdemir, E. M.; Polat, D. Cicek; Yoltas, A.; Biceroglu, O.; N/A; Karahüseyinoğlu, Serçin; Faculty Member; School of Medicine; 110772Laurocerasus officinalis Roem. (syn: Prunus laurocerasus L.) is a member of Rosaceae family. We investigated the antimicrobial and antioxidant activity of L. officinalis Roem in wound healing both in vivo and in vitro using an excisional wound model model in mice. We used four groups of eight mice as follows: untreated (control), empty gel, extract +gel (L. officinalis +gel), and Madecassol (R) groups. All treatments were applied topically once daily. The scar area, percentage wound closure and epithelization time were measured. L. officinalis promoted wound healing and increased granulation tissue, epidermal regeneration and angiogenesis. L. officinalis extract, which is known for its antioxidant and antimicrobial activities, may be useful for promoting wound healing.Publication Metadata only The rhythmicity of life: A review of the circadian clocks(Begell House Inc., 2021) Acikgoz, E.; Ayla, S.; Oktem, G.; N/A; Karahüseyinoğlu, Serçin; Faculty Member; School of Medicine; 110772Physiology of the mammalian body has been adapted to diurnal cycles of around 24 h, an evolutionary situation that affects a wide spectrum of biological events including sleep-to-wake transitions, feeding/fasting, body temperature, and hormonal regulations. The patterns of the diurnal cycle occur due to rhythmic oscillations that arise from the suprachiasmatic nucleus of hypothalamus, which also can be defined as the pacemaker of the system. The clock can be defined as a molecular machinery driven by the core clock genes that encode clock proteins in a rhythmic oscillatory fashion maintained by the light/dark cycles of the environment. Although the well-established knowledge refers to the function of the circadian rhythm as maintenance of the normal physiology, growing evidence shows that disruptions in the system usually caused by genetic and/or epigenetic misregulations may have a direct effect to lead major pathological conditions, such as carcinogenesis. This review outlines the main molecular aspects of circadian physiology, and reveals the reasons for and results of the circadian disruptions at different levels. In spite of the fact that more proof is needed for a direct correlation between circadian disruptions and oncogenesis and other pathological events, data obtained from current research supports the role of circadian rhythms in malfunctioning of the normal cellular metabolism.Publication Metadata only Investigation of the effects of tumor necrosis factor-alpha, H2o2, ethanol and butyrate on intestinal epithelial cell permeability(Wiley, 2019) Yazıcı, Duygu; Süer, Hande; Yılmaz, Özlem; Karahüseyinoğlu, Serçin; Saçkesen, Cansın; PhD Student; PhD Student; Master Student; Faculty Member; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; Graduate School of Health Sciences; Graduate School of Sciences and Engineering; School of Medicine; School of Medicine; N/A; N/A; N/A; 110772; 182537N/APublication Metadata only Comparing glutamatergic neuron population in the mediodorsal thalamic nucleus of genetic absence epilepsy rats from strasbourg (GAERS) and normal control Wistar rats(Elsevier Science Bv, 2016) Kirazli, Özlem; Onat, Filiz; N/A; N/A; N/A; Çavdar, Safiye; Özgür, Merve; Karahüseyinoğlu, Serçin; Faculty Member; PhD Student; Faculty Member; School of Medicine; Graduate School of Health Sciences; School of Medicine; Koç University Hospital; 1995; 197462; 110772An imbalance between GABAergic inhibition and glutamatergic excitation is suspected to play a role in the genesis of epileptic processes. In the present study we quantified the number of glutamate+ve neurons in the mediodorsal thalamic nucleus (MD) of genetic absence epilepsy rats from Strasbourg (GAERS) and compared these with values for normal Wistar rats. The MD thalamic nucleus was removed from each animal and the glutamatergic neurons were labelled using light-microscopy glutamate immunohistochemistry. The disector method was used to quantify the glutamate+ve neurons in the MD thalamic nucleus of GAERS and Wistar rats. The data were statistically analyzed. In the Wistar animals glutamate+ve neurons formed 89% and in GAERS 92.3% of the total neurons in 1000 mu m(3) of MD thalamic nucleus. In GAERS glutamate+ve neurons showed statistically significant increase in the MD thalamic nucleus compared to Wistar animals. In Wistar animals the glutamate-ve neurons formed 11% and in GAERS 7.7% of the total neurons in 1000 mu m(3) of MD thalamic. No significant difference was observed in glutamate ye neurons between the two strains. The average diameter of glutamate-ve neurons showed no significance, while glutamate-ve neurons were significant between the two strains. The results of the present study, on genetic absence epilepsy model, GAERS, confirms the role of MD thalamic nucleus in chemically induced absence epilepsy. (C) 2016 Elsevier B.V. All rights reserved.Publication Metadata only Construction of gel based 3D endometrial co -culture systems: can human mesenchymal stem cells be an alternative?(Oxford Univ Press, 2017) Yücel, D.; N/A; Karahüseyinoğlu, Serçin; Şahin, Gizem Nur; Şevgin, Kübra; Kocabay, Ahmet; Taşkın, Ali Cihan; Faculty Member; Master Student; PhD Student; Other; Other; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; Graduate School of Health Sciences; Graduate School of Health Sciences; N/A; N/A; 110772; N/A; N/A; N/A; 291296N/APublication Metadata only Polymeric and collagen biomaterials enhance implantation of mouse blastocysts in three-dimensional culture models(Elsevier, 2021) Başoz, Deniz; Yücel, Deniz; N/A; N/A; N/A; N/A; N/A; Department of Industrial Engineering; N/A; Department of Industrial Engineering; Ergün, Yağmur; Şahin, Gizem Nur; Şevgin, Kübra; Kocabay, Ahmet; Taşkın, Ali Cihan; Gönen, Mehmet; Karahüseyinoğlu, Serçin; PhD Student; PhD Student; PhD Student; Other; Other; Faculty Member; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; Graduate School of Health Sciences; Graduate School of Health Sciences; N/A; N/A; College of Engineering; School of Medicine; N/A; N/A; N/A; N/A; 291296; 237468; 110772