Researcher: Büyüktiryaki, Ayşe Betül
Name Variants
Büyüktiryaki, Ayşe Betül
Email Address
Birth Date
16 results
Search Results
Now showing 1 - 10 of 16
Publication Open Access Development of a low allergenic product for patients with milk allergy and assessment of its specific IgE reactivity(Wiley, 2024) Nemutlu, Emirhan; Kocak, Engin; Yazıcı, Duygu; Süer, Hande; Bülbüloğlu, Cemre Naz; Güzar, Elif; Büyüktiryaki, Ayşe Betül; Saçkesen, Cansın; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; School of Medicine;Background: Milk oral immunotherapy is the riskiest and most unpredictable form of oral immunotherapy. We aimed to produce a low allergenic product than conventional once baked-cake/muffin, to develop indirect in-house ELISA to check the tolerance status with milk products and evaluate IgE reactivity of patients' sera via western blotting (WB) and indirect in-house ELISA. Method: A low allergenic product named biscotti-twice baked-cake was developed, and the total protein concentration was determined. The protein content was studied by SDS-PAGE and proteomics. Milk-specific IgE (sIgE) binding assays were performed by WB and indirect in-house ELISA by using patients' sera. Results: Casein band intensity was observed to be lower in the biscotti-twice baked-cake than in the once baked-cake (p = .014). Proteomics analysis and alpha S1-casein measurement showed that the lowest intensity of casein was found in biscotti. The low binding capacity of milk sIgE to biscotti compared with once baked-cake was shown by WB (p = .0012) and by indirect in-house ELISA (p = .0001). In the ROC analysis, the area under the curve (AUC) of the in-house ELISA IgE was comparable with Uni-CAP milk and casein sIgE. The AUC of the in-house ELISA IgE for cake (0.96) and biscotti (1) was slightly better than Uni-CAP milk sIgE (0.94; 0.97) and casein sIgE (0.96; 0.97), respectively. Conclusion: The low allergenicity of the newly developed low allergenic product "biscotti-twice baked-cake" has been demonstrated by in vitro experiments. Biscotti could be a safe treatment option than once baked-cake/muffin in patients who are reactive to once baked-milk products.Publication Metadata only The association of milk and multiple food avoidance with growth parameters in infants and children(American College of Allergy, Asthma and Immunology, 2024) Eltan, Sevgi Bilgiç; Karakoç-Aydıner, Elif; Yılmaz, Ebru Arık; Can, Ceren Özel, Hülya Gökmen; Saçkesen, Cansın; Büyüktiryaki, Ayşe Betül; Gökçe, Tuğba; Göğebakan, Emre; Gündoğdu, Beliz Su; Cengiz, Hilal; Çelik, Nevin; İncir, Said; Yeşiltepe Mutlu, Rahime Gül; Yıldırım, Damla; Hatun, Şükrü; School of MedicineBackground: Recent studies reported that strict avoidance of milk products in cow's milk allergy (CMA) affects growth and bone turnover, causing negative calcium balance and changes in bone metabolism. Objective: To investigate biochemical parameters to predict bone turnover and its relations with height and weight measurements and nutritional intake. Methods: Height, weight, and body mass index z scores were plotted for age according to the World Health Organization. A 3-consecutive day food record was analyzed for nutritional values of foods. The blood levels of calcium, phosphorus, alkaline phosphatase, vitamin D, and parathyroid hormone (PTH) were determined. Results: The study included 69 controls, 66 children with isolated CMA, and 59 children with multiple food allergy (FA). The z scores for weight, height, and body mass index were lower in isolated CMA and multiple FA groups than controls (P < .001, P = .004, and P = .002, respectively). The nutritional intakes of protein, fat, carbohydrates, vitamins B2 and B12, niacin, calcium, and phosphorus were significantly lower in isolated CMA and multiple FA than controls. In infants (≤2 years of age), although blood calcium level was in normal range, it was significantly lower in isolated CMA and multiple FA than in controls (P < .001). In children older than 2 years, PTH level was significantly higher in isolated CMA and multiple FA groups than in controls (P = .003). Conclusion: Our study revealed that children with isolated CMA and multiple FA had a high nutrition gap, growth deceleration, and unbalanced bone metabolism, as illustrated by low blood calcium and elevated PTH levels. © 2024 American College of Allergy, Asthma & ImmunologyPublication Metadata only Clinical features, severity, and immunological changes during venom immunotherapy in children and adults(Ocean Side Publications, 2024) Özturk, Ayse Bilge; Dursun, Adile Berna; Donmez, Halil; Gelincik, Asli; Yegit, Osman Ozan; Yavuz, Suleyman Tolga; Sahiner, Umit Murat; Albayrak, Ozgur; Damadoglu, Ebru; Erdogan, Tuba; Firtina, Sinem; Taylan, Dilber; Soyer, Ozge; Karakaya, Gul; Kalyoncu, Ali Fuat; Sekerel, Bulent; Büyüktiryaki, Ayşe Betül; Hela, Francesko; Saçkesen, Cansın; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); School of Medicine; Graduate School of Health SciencesBackground: Hymenoptera venom allergy (HVA) is among the most common causes of severe allergic reactions worldwide. Objective: To investigate clinical features and factors that affect the severity of HVA and to determine the alterations in immunologic biomarkers after venom immunotherapy (VIT). Methods: Seventy-six adults and 36 children were prospectively investigated. We analyzed specific fic immunoglobulin E (sIgE) and sIgG4 levels of venom extracts and components (rApi m1, rApi m10, rVes v1, rVes v5, rPol d5) before and after the first year of VIT. Results: Although cardiovascular symptoms were more common in adults (p < 0.001), the skin was the most affected organ in children (p = 0.009). Serum basal tryptase (sBT) levels were higher in the adults than the children (p < 0.001). The absence of urticaria (odds ratio [OR] 4.208 [95% confidence interval {CI}, 1.395-12.688];p = 0.011) and sBT >= 5.2 ng/mL (OR 11.941 [95% CI, 5.220-39.733];p <0.001) were found as the risk factors for grade IV reactions. During VIT, changes in sIgE levels were variable. In the Apis VIT group, we observed remarkable increases in sIgG4 levels in Apis extract and rApi m1 but not in Api m10. Vespula extract, rVes v1, and rVes v5 sIgG4 levels were significantly increased in Vespula VIT group, we also detected significant increases in the Polistes extract and rPol d5 sIgG4 levels, which were not observed in the Apis VIT group. In the patients who received both Apis and Vespula VIT, increases in sIgG4 levels were observed for both venoms. Conclusion: Adults and children can have different clinical patterns. After 1 year, VIT induced a strong IgG4 response. Although Apis immunotherapy (IT) induced Apis sIgG4, excluding Api m10, Vespula IT induced both Vespula and Polistes sIgG4.Publication Metadata only The frequency of sleep-disordered breathing in preschool children with asthma and its effects on control of asthma(Codon Publications, 2024) Capanoglu, Murat; Ginis, Tayfur; Guvenir, Hakan; Vezir, Emine; Misirlioglu, Emine Dibek; Toyran, Muge; Ersu, Refika; Kocabas, Can Naci; Civelek, Ersoy; Büyüktiryaki, Ayşe Betül; School of Medicine; Koç University HospitalSleep-disordered breathing (SDB) is more common in asthmatic patients than in non-asthmatic persons, and SDB affects negatively to control asthma. A limited number of studies are discovered on the effect of SDB in preschool asthmatic children. In this study, we aimed to investigate the prevalence of SDB and its effect on control and severity of asthma in preschool children. A pediatric sleep questionnaire was completed by parents of asthmatic children. Patients who received a score of 0.33 or higher were diagnosed with SDB. Control and severity of asthma was assessed by a pediatric allergy specialist based on the Global Initiative for Asthma (GINA) criteria. The study included 249 patients, with a mean +/- SD age of 4.37 +/- 1.04 (range: 2-5.9) years;69% were boys;56.6% children had uncontrolled asthma and 28.7% had SDB. The SDB score was significantly different between controlled and uncontrolled asthma (0.19 vs 0.28;P < 0.001). The frequency of uncontrolled asthma in patients with and without SDB was 74.3% and 49.4%, respectively (P < 0.010). Based on the severity of asthma, the frequency of SDB among patients with mild, moderate, and severe asthma was 23.4%, 35.2%, and 47.4%, respectively (P = 0.010). Conclusion: The frequency and score of SDB were higher in patients with uncontrolled asthma. Frequency and score of SDB were significantly affected by the severity of asthma. SDB must be evaluated in preschool children with uncontrolled asthma.Publication Open Access Clinical characteristics and prognosis of legume allergy in children(Bilimsel Tıp Yayınevi) Brohi, Zeliha Yanginlar; Guvenir, Hakan; Celik, Ilknur Kulhas; Toyran, Muge; Civelek, Ersoy; Ginis, Tayfur; Kocabas, Can Naci; Misirlioglu, Emine Dibek; Büyüktiryaki, Ayşe Betül; Faculty Member; School of Medicine; 195944Objective: The knowledge concerning allergy to legumes is limited. We aimed to evaluate the clinical features and prognosis of legume allergy in children.Materials and Methods: We evaluated patients with legume allergy who were followed up from 2010 to 2017 at the Division of Pediatrics Allergy and Immunology, with their clinical features, laboratory findings, and prognosis.Results: The median age of the enrolled 37 patients in our study was 7 (interquartile range, 4.3-9.2) years. Twenty-nine (78.3%) were male. Thirteen (35.1%) patients were found to have an allergic reaction against more than one legume. The distribution of legume allergies was as follows: peanut (n=21, 56.8%), lentil (n=16, 43.2%), chickpea (n=13, 35.1%), pea (n=6,16.2%), bean (n=5, 13.5%), lupine (n=2, 5.4%), and kidney bean (n=1, 2.7%), with a total of 64 allergic reactions. The distribution of these different legume allergy reactions was as follows: urticaria and angioedema (n=31, 48.4%), anaphylaxis (n=23, 35.9%), atopic dermatitis (n=6, 9.3%), eosinophilic esophagitis (n=3, 7.8%), and food protein-induced enterocolitis syndrome (n=1, 1.5%). Thirty-two (86.5%) of 37 patients had an allergy to a non-legume food. Tolerance to 50 legume allergies affecting 27 patients being followed up for more than 12 months were given. Eight of the 18 patients with a single legume allergy and 1 of the 9 patients who were allergic to multiple legumes developed tolerance.Conclusion: Peanut and lentil were the most frequent legumes that caused allergic reactions in our study. The rate of allergies to non-legume foods was high. In patients who were allergic to a single legume, the tolerance rate was 44.4%.Publication Metadata only The common triggers of urticaria in children admitted to the pediatric emergency room(Wiley, 2022) Bezirganoğlu, Handan; Yılmaz, Ebru Arık; Şahiner, Ümit M.; Soyer, Özge; Şekerel, Bülent E.; Tekşam, Özlem; Büyüktiryaki, Ayşe Betül; Saçkesen, Cansın; Faculty Member; Faculty Member; School of Medicine; School of Medicine; 195944; 182537Background Urticaria frequently causes pediatric emergency department (PED) admissions. Children with urticaria may unnecessarily avoid suspected allergens. We aimed to investigate the possible and exact triggers of urticaria in children admitted to the PED. Methods Medical records of children admitted to the PED within a 1-year period were evaluated for the International Classification of Diseases 10 (ICD-10) L50 urticaria code, noting symptoms, and possible triggers of urticaria. We performed telephone interviews to complete the missing data and further diagnostic tests for IgE-mediated allergies to identify the exact triggers of urticaria. Results Among 60,142 children, 462 (0.8%) with the L50 code were evaluated. Possible triggers based on the history and physical examination could be identified in 46%: infections (18%), drugs (11%), foods (8%), infections and drugs (3%), insects (3%), pollen (1%), blood products (0.4%), and vaccines (0.4%). The most frequent infections related to urticaria were upper respiratory tract infections (74.5%), urinary tract infections (13.2%), gastroenteritis (8.2%), and otitis media (4.1%). After a diagnostic workup, IgE-mediated allergic diseases were diagnosed in 6% of patients. Twenty-two percent of the patients had multiple PED admission for the same urticaria flare. Urticaria severity was found to be the most important risk factor for readmissions to the PED (odds ratio: 3.86; 95% confidence interval: 2.39-6.23; p < .001). No relationship between urticaria severity, duration, and the triggers was present. Conclusions Despite detailed diagnostic tests, IgE-mediated allergic triggers were rarely the cause of urticaria in children admitted to the PED. Infections are the most frequent trigger. Severe urticaria causes more frequent readmissions to the PED.Publication Metadata only Development of a new hypoallergenic casein product for use in milk immunotherapy(Wiley, 2021) Büyüktiryaki, Ayşe Betül; Saçkesen, Cansın; N/A; Doctor; Faculty Member; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); N/A; N/A; School of Medicine; Koç University Hospital; N/A; 195944; 182537N/APublication Metadata only Food allergy severity predictions based on cellular in vitro tests(Taylor & Francis, 2020) Buyuktiryaki, Betul; Santos, Alexandra F.; Büyüktiryaki, Ayşe Betül; Faculty Member; School of Medicine; 195944Introduction Food allergy is increasing in prevalence and the severity of allergic reactions is unpredictable. Identifying food-allergic patients at high risk of severe reactions would allow us to offer a personalized and improved management for these patients. Areas covered We review the evidence for using the levels of specific IgE, the nature of the allergen, and cellular tests to identify patients at high risk of developing severe allergic reactions to foods. Expert opinion The evidence about whether the quantity of allergen-specific IgE reflects the severity of allergic reactions to foods is conflicting, with some positive and some negative studies. For some foods, specific IgE to individual components (e.g. Ara h 2 in peanut) can provide additional information. However, more important than the quantity of IgE is possibly the quality of IgE, which can be captured by individual measurements of affinity/avidity, diversity, and specific activity, but is best measured overall using the basophil and mast cell activation tests, which assess the function of IgE in its ability to induce cell activation, degranulation, and mediator release. Biomarkers look at a single aspect of the allergic response and should be interpreted in the broader clinical context for each individual patient assessed.Publication Open Access IgE-mediated Anisakis allergy in children(Codon Publications, 2023) Pontone Matteo; Giovannini Mattia; Barni Simona; Mori Francesca; Venturini Elisabetta; Galli Luisa; Valleriani Claudia; De las Vecillas Leticia; Lopata Andres Ludwig; Saçkesen, Cansın; Büyüktiryaki, Ayşe Betül; Faculty Member; Faculty Member; School of Medicine; School of Medicine; 182537; 195944Anisakids are nematodes responsible for different clinical patterns in humans. The well-known human-infecting Anisakis species include members of the Anisakis simplex (AS) complex. Humans usually contract anisakiasis through ingestion of raw or undercooked seafood containing Anisakis larvae. Once Anisakis has been ingested, patients may develop disease driven directly by Anisakis larvae and/or by allergic reaction due to this nematode. The capability of inducing allergic reactions depends on the expression of specific antigens by nematodes and host factors. This study aims to resume actual knowledge about AS and Anisakiasis with regard to epidemiology, pathophysiology, clinical presentation, diagnosis, and treatment. Particular attention is paid to Anisakis allergens and their cross-reactivity on available diagnostic methods, and defining a diagnostic pathway for Anisakis allergy. Because only a few data are available in the literature about pediatric population, we focus on this group of patients specifically © 2023 Codon Publications. Published by Codon PublicationsPublication Open Access Allergic contact dermatitis to temporary black henna tattoo due to sensitization to para-phenylenediamine(Buluş Design, 2021) Dibek Mısırlıoğlu, Emine; Tanıdır, Merve; Kanık Yüksek, Saliha; Şahiner, Neriman; Güvenir, Hakan; Azkur, Dilek; Kocabaş, Can Naci; Büyüktiryaki, Ayşe Betül; Faculty Member; School of MedicineThe fashion for temporary ""black henna tattoo"" is becoming increasingly more common among young adults and teens. An adolescent case with itching, erythema, edema, and inflammatory discharge on the right forearm two days after a temporary tattoo application is presented in this report. To determine the contact sensitizer, a patch test was performed one month after the end of the treatment. A bullous strong positive reaction (3+) was observed against para-phenylenediamine at the 48th and 72nd hours of evaluation after the application. Despite the common belief that henna tattoo application is harmless, the ingredients used in henna tattoo especially paraphenylenediamine can lead to allergenic reactions that can even be severe.