Researcher:
Akolpoğlu, Mükrime Birgül

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Master Student

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Mükrime Birgül

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Akolpoğlu

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Akolpoğlu, Mükrime Birgül

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2018 - 201942020 - 20212

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Researcher Of Publications: search.filters.isAuthorOfPublication.6d83a0d0-2b21-4948-ab9e-3bc1ca48534d

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Now showing 1 - 6 of 6
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    Biosensing–drug delivery systems for in vivo applications
    (Elsevier, 2019) Erkoc, Pelin; N/A; N/A; Department of Chemical and Biological Engineering; Akolpoğlu, Mükrime Birgül; Bozüyük, Uğur; Kızılel, Seda; Master Student; PhD Student; Faculty Member; Department of Chemical and Biological Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Engineering; N/A; N/A; 28376
    Early detection of diseases can increase the efficiency of therapies. Recent advances in biosensor technology have led to the development of accurate and robust systems that can sense disease-dependent changes in analyte. These advancements have enabled faster diagnosis and treatment for various diseases. The invention of smart-responsive materials has opened new gates for next-generation biosensors, which can release the therapeutic payload upon environmental changes. These integrated systems provide increased therapeutic efficacy with reduced side effects, and a better perspective for biomedical applications. With further efforts, including comprehensive research and computational modeling, biosensing–drug delivery systems may become powerful tools for the treatment of chronic diseases.
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    An all-aqueous approach for physical immobilization of PEG-lipid microgels on organoid surfaces
    (Elsevier, 2020) N/A; N/A; Department of Chemical and Biological Engineering; Akolpoğlu, Mükrime Birgül; İnceoğlu, Yasemin; Kızılel, Seda; Master Student; Master Student; Faculty Member; Department of Chemical and Biological Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Engineering; N/A; N/A; 28376
    Emulsion-based generation of hydrogel particles has been widely explored for numerous applications in fields such as biomedical, food, and drug delivery. Water-in-water emulsion (w/w) is an organic solvent-free approach and exploits solely aqueous media to generate nano- or micropartides. This strategy is environment-friendly and favorable for biomedical applications where biocompatibility is the ultimate criterion. Hence, PEG-based microgels can be synthesized with desired size and functionality using w/w emulsion technique. To estimate the influence of emulsification parameters on size and stability of PEG-lipid microgels, optimizations using three independent input variables were carried out: (i) ultrasonication power, (ii) ultrasonication duration, and (iii) duration of light exposure. Physical immobilization of microgels on islet-organoids was achieved through hydrophobic interactions. Cell function and viability were assessed thoroughly after microgel immobilization. Microgel size is dependent on ultrasonication parameters and microgel stability is vastly determined by the duration of light exposure. Immobilization of microgels with 5 mM lipid moiety promoted coating of islet-organoids. Coated organoids retained their function and viability without significant adverse effects. This is important for understanding fundamental aspects of PEG-lipid microgels using w/w emulsion, useful for possible drug/gene delivery applications to increase treatment efficiency and ultimately lead to clinical translation of PEG microgels for biomedical applications.
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    Calcification resistance of polyisobutylene and polyisobutylene-based materials
    (Wiley, 2019) Kekeç, Nur Çiçek; Nugay, Nihan; Nugay, Turgut; Kennedy, Joseph P.; N/A; N/A; Department of Chemical and Biological Engineering; Akolpoğlu, Mükrime Birgül; Bozüyük, Uğur; Kızılel, Seda; Master Student; PhD Student; Faculty Member; Department of Chemical and Biological Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Engineering; N/A; N/A; 28376
    Calcification of implanted biomaterials is highly undesirable and limits clinical applicability. Experiments were carried out to assess the calcification resistance of polyisobutylene (PIB), PIB-based polyurethane (PIB-PU), PIB-PU reinforced with (CH3)(3)N+CH2CH2CH2NH2 I--modified montmorillonite (PIB-PU/nc), PIB-based polyurethane urea (PIB-PUU), PIB-PU containing S atoms (PIBS-PU), PIBS-PU reinforced with (CH3)(3)N+CH2CH2CH2NH2 I--modified montmorillonite (PIBS-PU/nc), and poly(isobutylene-b-styrene-b-isobutylene) (SIBS), relative to that of a clinically widely implanted polydimethylsiloxane (PDMS)-based PU, Elast-Eon (the "control"). Samples were incubated in simulated body fluid for 28 days at 37 degrees C, and the extent of surface calcification was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), energy-dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), and Fourier-transform-infrared (FT-IR) spectroscopy. Whereas the PDMS-based PU showed extensive calcification, PIB and PIB-PU containing 72.5% PIB, ie, a polyurethane whose surface is covered with PIB, were free of calcification. PIBS-PU and PIB-PUU, ie, polyurethanes that contain S or urea groups, respectively, were slightly calcified. The amine-modified montmorillonite-reinforcing agent reduced the extent of calcification. SIBS was found slightly calcified. Evidently, PIB and materials fully coated with PIB are calcification resistant.
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    Recent advances in the design of implantable insulin secreting heterocellular islet organoids
    (Elsevier Sci Ltd, 2021) Sousa, Ana Rita; Oliveira, Mariana B.; Mano, Joao F.; N/A; N/A; N/A; Department of Chemical and Biological Engineering; Akolpoğlu, Mükrime Birgül; İnceoğlu, Yasemin; Bozüyük, Uğur; Kızılel, Seda; Master Student; Master Student; PhD Student; Faculty Member; Department of Chemical and Biological Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Engineering; N/A; N/A; N/A; 28376
    Islet transplantation has proved one of the most remarkable transmissions from an experimental curiosity into a routine clinical application for the treatment of type I diabetes (T1D). Current efforts for taking this technology one-step further are now focusing on overcoming islet donor shortage, engraftment, prolonged islet availability, post-transplant vascularization, and coming up with new strategies to eliminate lifelong immunosuppression. To this end, insulin secreting 3D cell clusters composed of different types of cells, also referred as heterocellular islet organoids, spheroids, or pseudoislets, have been engineered to overcome the challenges encountered by the current islet transplantation protocols. beta-cells or native islets are accompanied by helper cells, also referred to as accessory cells, to generate a cell cluster that is not only able to accurately secrete insulin in response to glucose, but also superior in terms of other key features (e.g. maintaining a vasculature, longer durability in vivo and not necessitating immunosuppression after transplantation). Over the past decade, numerous 3D cell culture techniques have been integrated to create an engineered heterocellular islet organoid that addresses current obstacles. Here, we first discuss the different cell types used to prepare heterocellular organoids for islet transplantation and their contribution to the organoids design. We then introduce various cell culture techniques that are incorporated to prepare a fully functional and insulin secreting organoids with select features. Finally, we discuss the challenges and present a future outlook for improving clinical outcomes of islet transplantation.
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    PublicationOpen Access
    Engineering human stellate cells for beta cell replacement therapy promotes in vivo recruitment of regulatory T cells
    (Elsevier, 2019) N/A; Department of Chemical and Biological Engineering; Oran, Dilem Ceren; Lokumcu, Tolga; Bal, Tuğba; İnceoğlu, Yasemin; Albayrak, Özgür; Erkan, Murat Mert; Kurtoğlu, Metin; Can, Füsun; Önder, Tuğba Bağcı; Kızılel, Seda; Akolpoğlu, Mükrime Birgül; Faculty Member; Faculty Member; Master Student; Department of Chemical and Biological Engineering; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Sciences and Engineering; Graduate School of Health Sciences; College of Engineering; School of Medicine; N/A; N/A; N/A; N/A; N/A; N/A; N/A; 103165; 184359; 28376; N/A
    Type 1 diabetes (T1D) is an autoimmune disease characterized by destruction of pancreatic β cells. One of the promising therapeutic approaches in T1D is the transplantation of islets; however, it has serious limitations. To address these limitations, immunotherapeutic strategies have focused on restoring immunologic tolerance, preventing transplanted cell destruction by patients’ own immune system. Macrophage-derived chemokines such as chemokine-ligand-22 (CCL22) can be utilized for regulatory T cell (Treg) recruitment and graft tolerance. Stellate cells (SCs) have various immunomodulatory functions: recruitment of Tregs and induction of T-cell apoptosis. Here, we designed a unique immune-privileged microenvironment around implantable islets through overexpression of CCL22 proteins by SCs. We prepared pseudoislets with insulin-secreting mouse insulinoma-6 (MIN6) cells and human SCs as a model to mimic naive islet morphology. Our results demonstrated that transduced SCs can secrete CCL22 and recruit Tregs toward ​the implantation site in vivo. This study is promising to provide a fundamental understanding of SC-islet interaction and ligand synthesis and transport from SCs at the graft site for ensuring local immune tolerance. Our results also establish a new paradigm for creating tolerable grafts for other chronic diseases such as diabetes, anemia, and central nervous system (CNS) diseases, and advance the science of graft tolerance.
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    PublicationOpen Access
    Anti-icing properties on surfaces through a functional composite: effect of ionic salts
    (American Chemical Society (ACS), 2018) Department of Mathematics; Aydın, Derya; Akolpoğlu, Mükrime Birgül; Kızılel, Rıza; Kızılel, Seda; Researcher; Master Student; Researcher; Faculty Member; Department of Mathematics; Koç University Tüpraş Energy Center (KUTEM) / Koç Üniversitesi Tüpraş Enerji Merkezi (KÜTEM); College of Engineering; Graduate School of Sciences and Engineering; College of Sciences; N/A; N/A; N/A; 28376
    This study reports the potential of a unique functional composite for anti-icing applications. To date, various ionic salt formulations have been applied to prevent ice accumulation on surfaces. However, salt can be removed by external factors and large amounts must be used to attain anti-icing properties. Incorporating hydrophilic salts into hydrophobic mediums and controlled release of specific agents can provide effective solution to reduce ice accumulation on surfaces. Here, we developed functional polymer composites with salt pockets of altered ionic salts consisting of potassium formate (KCOOH), sodium chloride (NaCl), or magnesium chloride (MgCl2). We dissolved ionic salts in hydrophilic gel domains and dispersed in a hydrophobic styrene-butadiene-styrene polymer matrix. Na+ and Cl- ions delayed ice formation by 42.6 min at -2 degrees C compared to that for unmodified surfaces. Functional composites prepared with the NaCl ionic salt exhibited better anti-icing behavior at -2 degrees C because of their high concentration compared to that of the composites prepared with KCOOH and MgCl2 ionic salts. We also characterized the release of ionic salts from composite-modified hydrophobic medium separately up to 118 days. Furthermore, we monitored freezing of water on composite-incorporated or composite-coated hydrophobic surfaces in a camera-integrated cold chamber with a uniform temperature (-2 degrees C). The results demonstrated significant increases in the delay of freezing on composite-incorporated or composite-coated surfaces compared to that on controls. We observed altered effects of each ionic salt on the mechanical, morphological, and functional properties of the composite-incorporated or composite-coated hydrophobic surfaces. Our results suggested that the efficiency of a polymer composite to promote anti-icing behavior on a surface is directly related to the type and concentration of the particular ionic salt incorporation into the composite. This approach is promising and demonstrates significant potential of the ionic salt embedded within polymer composite-modified hydrophobic surfaces to attain delayed icing function.