Researcher:
Bayam, Efil

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Efil

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Bayam

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Bayam, Efil

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2014 - 201932020 - 20211

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Researcher Of Publications: search.filters.isAuthorOfPublication.cebdf59b-afe4-4889-917d-7471a5d755cb

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    NEUROD2 transcription factor regulates expression of Id2 gene during neuronal differentiation
    (Wiley-Blackwell, 2014) N/A; N/A; N/A; Department of Molecular Biology and Genetics; Güzelsoy, Gizem; Bayam, Efil; Dunn, Gülayşe İnce; Master Student; Researcher; Other; Department of Molecular Biology and Genetics; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; N/A; N/A; N/A
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    NEUROD2 regulates stim1 expression and store-operated calcium entry in cortical neurons
    (Soc Neuroscience, 2017) N/A; N/A; N/A; N/A; N/A; N/A; N/A; N/A; Department of Physics; Department of Molecular Biology and Genetics; Güner, Gökhan; Güzelsoy, Gizem; İşleyen, Fatma Sadife; Şahin, Gülcan Semra; Akkaya, Cansu; Bayam, Efil; Kotan, Ilgın Eser; Kabakçıoğlu, Alkan; Dunn, Gülayşe İnce; Master Student; Master Student; Master Student; Master Student; PhD Student; Researcher; Master Student; Faculty Member; Other; Department of Physics; Department of Molecular Biology and Genetics; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; College of Sciences; N/A; N/A; N/A; N/A; N/A; N/A; N/A; 49854; N/A
    Calcium signaling controls many key processes in neurons, including gene expression, axon guidance, and synaptic plasticity. In contrast to calcium influx through voltage- or neurotransmitter-gated channels, regulatory pathways that control store-operated calcium entry (SOCE) in neurons are poorly understood. Here, we report a transcriptional control of Stim1 (stromal interaction molecule 1) gene, which is a major sensor of endoplasmic reticulum (ER) calcium levels and a regulator of SOCE. By using a genome-wide chromatin immunoprecipitation and sequencing approach in mice, we find that NEUROD2, a neurogenic transcription factor, binds to an intronic element within the Stim1 gene. We show that NEUROD2 limits Stim1 expression in cortical neurons and consequently fine-tunes the SOCE response upon depletion of ER calcium. Our findings reveal a novel mechanism that regulates neuronal calcium homeostasis during cortical development.
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    Roles of developmentally regulated KIF2A alternative isoforms in cortical neuron migration and differentiation
    (The Company of Biologists, 2021) N/A; N/A; N/A; N/A; N/A; N/A; N/A; Department of Molecular Biology and Genetics; N/A; Akkaya, Cansu; Atak, Dila; Kamacıoğlu, Altuğ; Akarlar, Büşra; Güner, Gökhan; Bayam, Efil; Taşkın, Ali Cihan; Dunn, Gülayşe İnce; PhD Student; PhD Student; Master Student; Other; Master Student; Researcher; Other; Faculty Member; Other; Department of Molecular Biology and Genetics; N/A; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; N/A; Graduate School of Sciences and Engineering; N/A; N/A; College of Sciences; N/A; N/A; N/A; N/A; N/A; N/A; N/A; 291296; 105301; N/A
    KIF2A is a kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. However, how different KIF2A alternative isoforms function during development of the cerebral cortex is not known. Here, we focus on three Kif2a isoforms expressed in the developing cortex. We show that Kif2a is essential for dendritic arborization in mice and that the functions of all three isoforms are sufficient for this process. Interestingly, only two of the isoforms can sustain radial migration of cortical neurons; a third isoform, lacking a key N-terminal region, is ineffective. By proximity-based interactome mapping for individual isoforms, we identify previously known KIF2A interactors, proteins localized to the mitotic spindle poles and, unexpectedly, also translation factors, ribonucleoproteins and proteins that are targeted to organelles, prominently to the mitochondria. In addition, we show that a KIF2A mutation, which causes brain malformations in humans, has extensive changes to its proximity-based interactome, with depletion of mitochondrial proteins identified in the wild-type KIF2A interactome. Our data raises new insights about the importance of alternative splice variants during brain development.
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    PublicationOpen Access
    Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation
    (BioMed Central, 2015) Department of Molecular Biology and Genetics; Department of Physics; Bayam, Efil; Şahin, Gülcan Semra; Güzelsoy, Gizem; Güner, Pınar Tatar; Kabakçıoğlu, Alkan; Dunn, Gülayşe İnce; Teaching Faculty; Faculty Member; Department of Molecular Biology and Genetics; Department of Physics; College of Sciences; N/A; N/A; N/A; 188227; 49854; N/A
    Background: Cellular differentiation programs are controlled, to a large extent, by the combinatorial functioning of specific transcription factors. Corticalprojection neurons constitute the major excitatory neuron population within the cortex and mediate long distance communication between the cortex and other brain regions. Our understanding of effector transcription factors and their downstream transcriptional programs that direct the differentiation process ofcortical projection neurons is far from complete. Results: In this study, we carried out a ChIP-Seq (chromatin-immunoprecipitation and sequencing) analysis of NEUROD2, an effector transcription factor expressed in lineages of cortical projection neurons during the peak of cortical excitatory neurogenesis. Our results suggest that during cortical developmentNEUROD2 targets key genes that are required for Reelin signaling, a major pathway that regulates the migration of neurons from germinal zones to their final layers of residence within the cortex. We also find that NEUROD2 binds to a large set of genes with functions in layer-specific differentiation and in axonal pathfinding of cortical projection neurons. Conclusions: Our analysis of in vivo NEUROD2 target genes offers mechanistic insight into signaling pathways that regulate neuronal migration and axon guidance and identifies genes that are likely to be required for proper cortical development.