Publication:
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation

dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.departmentDepartment of Chemical and Biological Engineering
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorGül, Şeref
dc.contributor.kuauthorKaralar, Elif Nur Fırat
dc.contributor.kuauthorKavaklı, İbrahim Halil
dc.contributor.kuauthorOdabaşı, Ezgi
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.date.accessioned2024-11-09T11:51:44Z
dc.date.issued2019
dc.description.abstractCentriolar satellites are ubiquitous in vertebrate cells. They have recently emerged as key regulators of centrosome/cilium biogenesis, and their mutations are linked to ciliopathies. However, their precise functions and mechanisms of action remain poorly understood. Here, we generated a kidney epithelial cell line (IMCD3) lacking satellites by CRISPR/Cas9-mediated PCM1 deletion and investigated the cellular and molecular consequences of satellite loss. Cells lacking satellites still formed full-length cilia but at significantly lower numbers, with changes in the centrosomal and cellular levels of key ciliogenesis factors. Using these cells, we identified new ciliary functions of satellites such as regulation of ciliary content, Hedgehog signaling, and epithelial cell organization in three-dimensional cultures. However, other functions of satellites, namely proliferation, cell cycle progression, and centriole duplication, were unaffected in these cells. Quantitative transcriptomic and proteomic profiling revealed that loss of satellites affects transcription scarcely, but significantly alters the proteome. Importantly, the centrosome proteome mostly remains unaltered in the cells lacking satellites. Together, our findings identify centriolar satellites as regulators of efficient cilium assembly and function and provide insight into disease mechanisms of ciliopathies.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue6
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuEU - TÜBİTAK
dc.description.sponsorshipEuropean Research Council (ERC)
dc.description.sponsorshipEuropean Union (European Union)
dc.description.sponsorshipHorizon 2020
dc.description.sponsorshipEMBO Installation Grant
dc.description.sponsorshipNewton Advanced Fellowship
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TÜBİTAK)
dc.description.sponsorshipScience Academy of Turkey
dc.description.versionPublisher version
dc.description.volume20
dc.identifier.doi10.15252/embr.201947723
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR01845
dc.identifier.issn1469-221X
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85065010693
dc.identifier.urihttps://doi.org/10.15252/embr.201947723
dc.identifier.wos472972600001
dc.keywordsCentriolar satellites
dc.keywordsHedgehog
dc.keywordsPCM1
dc.keywordsPrimary cilium
dc.language.isoeng
dc.publisherWiley
dc.relation.grantno679140
dc.relation.grantnoRG84475
dc.relation.grantno115Z521 to ENF
dc.relation.ispartofEMBO Reports
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/8465
dc.subjectBiochemistry and molecular biology
dc.subjectCell biology
dc.titleCentriolar satellites are required for efficient ciliogenesis and ciliary content regulation
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorOdabaşı, Ezgi
local.contributor.kuauthorKaralar, Elif Nur Fırat
local.contributor.kuauthorGül, Şeref
local.contributor.kuauthorKavaklı, İbrahim Halil
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit1College of Engineering
local.publication.orgunit2Department of Molecular Biology and Genetics
local.publication.orgunit2Department of Chemical and Biological Engineering
local.publication.orgunit2Graduate School of Sciences and Engineering
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