Publication: Tlr4 asp299gly and thr399ile and tlr2 intron 2 microsatellite gene polymorphism in patients with acute biliary pancreatitis: does it cause the disease?
Program
KU-Authors
KU Authors
Co-Authors
Anılır, Ender
Özen, Filiz
Özemir, İbrahim Ali
Yıldırım, İbrahim Halil
Bilgiç, Çağrı
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Abstract
Objectives: There has been coverage of Toll-like receptor 4 and Toll-like receptor 2 gene polymorphisms in inflammatory episodes in a number of studies. In view of the inflammatory nature of acute pancreatitis, we aimed to determine the predictive value of mutations in Asp299Gly and Thr399Ile of the Toll-like receptor 4 gene, and the intron 2 microsatellite polymorphism of the Toll-like receptor 2 gene on the occurrence of acute biliary pancreatitis. Material and Methods: The study included 86 patients for the Toll-like receptor 4 Thr399Ile polymorphism, 100 patients for the Toll-like receptor 4 Asp299Gly polymorphism with acute biliary pancreatitis, and 101 healthy volunteers. At the same time, 93 patients and 92 healthy volunteers were included in the study to research the Toll-like receptor 2 intron 2 microsatellite polymorphism. Genotypes were determined using the restriction fragment length polymorphism analysis of PCR products and by an allele-specific PCR. Results: The Toll-like receptor 4 Thr399Ile homozygotes mutant variants (p=0.005) and Toll-like receptor 2 MM genotype (p<0.001) were detected with a significantly higher frequency in patients with acute biliary pancreatitis than in the healthy blood donors. Conclusion: The Toll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms and the Toll-like receptor 2 intron 2 microsatellite polymorphism are statistically associated with ABP.
Source
Publisher
Aves
Subject
Surgery
Citation
Has Part
Source
Turkish Journal of Surgery
Book Series Title
Edition
DOI
10.5152/turkjsurg.2017.3828