Publication:
Impact of PDMS surface treatment in cell-mechanics applications

dc.contributor.departmentDepartment of Mechanical Engineering
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentKUYTAM (Koç University Surface Science and Technology Center)
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.kuauthorDoğru, Sedat
dc.contributor.kuauthorAydemir, Duygu
dc.contributor.kuauthorSalman, Naveed
dc.contributor.kuauthorUlusu, Nuriye Nuray
dc.contributor.kuauthorAlaca, Burhanettin Erdem
dc.contributor.kuprofileMaster Student
dc.contributor.kuprofilePhD Student
dc.contributor.kuprofileOther
dc.contributor.kuprofileFaculty Member
dc.contributor.kuprofileFaculty Member
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.yokidN/A
dc.contributor.yokidN/A
dc.contributor.yokidN/A
dc.contributor.yokid6807
dc.contributor.yokid115108
dc.date.accessioned2024-11-09T22:57:45Z
dc.date.issued2020
dc.description.abstractAs a widely used elastomer in cell mechanics studies, PDMS is exposed to a variety of surface treatments during cell culture preparation. Considering its viscoelastic nature in particular, effects of the aforementioned treatments on PDMS mechanical behaviour, especially at the relevant length scale of 100 mu m, received limited attention. This is despite the fact that significant errors were reported in the quantification of cellular traction forces as a result of minute changes in PDMS mechanical properties. Hence, the effects of plasma oxidation, sterilization and incubation on PDMS modulus of elasticity, relaxation modulus and Poisson's ratio are studied here through tension and stress relaxation tests, with the results of the latter interpreted via the linear viscoelastic formulation. It is observed that although significant deviations from the properties of untreated PDMS are measured through this cycle of surface treatment, properties of untreated PDMS are almost recovered following incubation in cell medium. For example, the modulus of elasticity of treated PDMS was found to be 6% smaller than that of the untreated PDMS. The corresponding deviation was <3% and <1% for the relaxation modulus and time-averaged Poisson's ratio, respectively. The rate of change of the Poisson's ratio with time was also found to be reduced at the end of incubation process in cell medium. As a result, viscoelastic properties of untreated PDMS can safely be used within the error margins provided by this work.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuTÜBİTAK
dc.description.sponsorshipScientific and Technological Research Council of Turkey [116F397]
dc.description.sponsorshipPresidency of Turkey, Presidency of Strategy and Budget SD and BEA acknowledge support by the Scientific and Technological Research Council of Turkey under Grant No. 116F397 and invaluable support by Prof. Halil Bayraktar of Istanbul Technical University regarding image processing. DA and NNU gratefully acknowledge use of the services and facilities of the Koc University Research Center for Translational Medicine (KUTTAM), funded by the Presidency of Turkey, Presidency of Strategy and Budget.
dc.description.volume103
dc.identifier.doi10.1016/j.jmbbm.2019.103538
dc.identifier.eissn1878-0180
dc.identifier.issn1751-6161
dc.identifier.scopus2-s2.0-85075207851
dc.identifier.urihttp://dx.doi.org/10.1016/j.jmbbm.2019.103538
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7598
dc.identifier.wos517856400012
dc.keywordsPolydimethylsiloxane (PDMS)
dc.keywordsCell culture
dc.keywordsCell mechanics
dc.keywordsViscoelastic characterization
dc.keywordsRelaxation modulus
dc.keywordsPoisson's ratio
dc.keywordsTraction force microscopy
dc.keywordsPoissons ratio
dc.keywordsViscoelastic characterization
dc.keywordsInstrumented indentation
dc.keywordsTranscription factors
dc.keywordsMechanotransduction
dc.keywordsMechanobiology
dc.keywordsPolydimethylsiloxane
dc.keywordsExpression
dc.keywordsAdhesion
dc.languageEnglish
dc.publisherElsevier
dc.sourceJournal of the Mechanical Behavior of Biomedical Materials
dc.subjectEngineering
dc.subjectBiomedical engineering
dc.subjectMaterials science
dc.subjectBiomaterials
dc.titleImpact of PDMS surface treatment in cell-mechanics applications
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0002-6696-9362
local.contributor.authorid0000-0002-6449-2708
local.contributor.authorid0000-0002-0248-9490
local.contributor.authorid0000-0002-3173-1389
local.contributor.authorid0000-0001-5931-8134
local.contributor.kuauthorDoğru, Sedat
local.contributor.kuauthorAydemir, Duygu
local.contributor.kuauthorSalman, Naveed
local.contributor.kuauthorUlusu, Nuriye Nuray
local.contributor.kuauthorAlaca, Burhanettin Erdem
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1College of Engineering
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Mechanical Engineering
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2KUYTAM (Koç University Surface Science and Technology Center)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Sciences and Engineering
local.publication.orgunit2Graduate School of Health Sciences
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