Publication: Boric Acid Improved Cryopreserved Mouse Embryo Development
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KU-Authors
KU Authors
Co-Authors
askin, A. C.
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Type
Embargo Status
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Abstract
Boric acid (BA) is an essential trace element that is required to support the metabolic pathways in plants, humans, and animals. The present study investigates the in vitro development and quality of single-cell mouse embryos in a BA-added culture medium after cryopreservation using the solid-surface vitrification method. For this purpose, the pronuclear-stage embryos derived from superovulated C57Bl/6j mouse strains and the one-cell embryos were then cryopreserved using the solid-surface vitrification (SSV) method. After thawing, the embryos were cultured in a BA-added medium at 37 degrees C in a 5% CO2 environment until the blastocyst stage. The resulting in vitro development rates of the embryos in the control group, SSV group, and SSV + 1.62 x 10-4 mu M BA group were 68.11% (36/59), 40.16% (16/48), and 64.92% (28/48) respectively, indicating that the BA supported the in vitro development of the embryos cryopreserved using the SSV method. Our results suggest that the addition of boric acid to the culture media increased the development rate of the embryos that were vitrified using the SSV method.
Source
Publisher
Springernature
Subject
Biochemistry, Molecular biology
Citation
Has Part
Source
Biological Trace Element Research
Book Series Title
Edition
DOI
10.1007/s12011-023-03990-9