Activating hepatic progenitor stem cells to regenerate liver cells after liver damage induced by CCl(4) using targeting silymarin-loaded albumin/hyaluronic acid nanoparticles

Abstract

Stem cell nanotechnology (SCN) constitutes a pivotal domain of scientific inquiry aimed at facilitating stem cell-based investigations involving nanoparticles. At present, nanoparticles (NPs) can be synthesized from many sources using various methodologies, which may be categorized as physical, chemical, or biological, along with their distinct attributes such as morphology, dimensions, and electrostatic properties. Furthermore, it is important to advance the applications of green synthesis in the context of utilizing NPs within the biomedical sciences. To this end, there exists a requisite for the generation of NPs utilizing biological resources, followed by thorough characterization and exploration of their impacts on stem cells. This protocol determines the fabrication and characterization of silymarin NPs derived from the dried milk thistle plant as well as the assessment of their influence on the activation of hepatic progenitor stem cells and, by extension, the process of liver regeneration after hepatic damage induced by CCl4.