Protective effect and results of glutamine and partially hydrolyzed Guar gum on rats with experimental radiation enteritis

Abstract

The aim of our study is to evaluate the effects of glutamine, partially hydrolyzed guar gum and combined glutamine and partially hydrolyzed guar gum form in rats with experimental radiation enteritis by histopathological, immunohistochemical and biochemical measurements. 64 Wistar albino rats with an average weight of 220-280 g were used in the study. 1st group only took water, 2nd group only took glutamine, 3rd group only took partially hydrolyzed guar gum, 4th group took partially hydrolyzed guar gum with glutamine, 5th group took only radiotherapy, 6th group took radiotherapy with glutamine, 7th group took radiotherapy with partially hydrolyzed guar gum and 8th group took radiotherapy, glutamine and guar gum. Partially hydrolyzed guar gum was given with radiotherapy, and glutamine and guar gum were given together with radiotherapy in the 8th group. All rats were weighed on the first, seventh and tenth days. On the tenth day, the rats were sacrificed and their clinical status, biochemical, histopathological and immunohistochemical parameters in the terminal ileum were evaluated. Differences between groups were compared statistically. All groups were compared in terms of crypt length, villus height, crypt abscess, congestion, intraepithelial lymphoid infiltration, stool consistency, myeloperoxidase, malondialdehyde, caspase-3% and tumor necrosis factor alpha mean, and statistical significance was observed (p < 0.05). Weight% difference in rats was statistically significant between groups (p < 0.05). Interleukin-10 values were not statistically significant between groups (p > 0.05). In paired comparisons, there was a statistically significant difference between the control group and the group with radiation enteritis in terms of biochemical, histopathological, immunohistochemical and clinical parameters (p < 0.05). There was no statistically significant difference between the 5th group and 6th group, 7th group and 8th groups in terms of biochemical, histopathological, immunohistochemical and clinical parameters (p > 0,05). The result suggest that glutamine, partially hydrolyzed guar gum and combination therapy may not have a protective effect on radiation enteritis.