Publication:
Bisphosphonate-functionalized poly(amido amine) crosslinked 2-hydrox-yethyl methacrylate hydrogel as tissue engineering scaffold

dc.contributor.coauthorGüven, Melek Naz
dc.contributor.coauthorBalaban, Burcu
dc.contributor.coauthorOkay, Oğuz
dc.contributor.coauthorAvcı, Duygu
dc.contributor.departmentDepartment of Chemistry
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorDemirci, Gözde
dc.contributor.kuauthorAcar, Havva Funda Yağcı
dc.contributor.kuprofileMaster Student
dc.contributor.kuprofileFaculty Member
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.yokidN/A
dc.contributor.yokid178902
dc.date.accessioned2024-11-09T23:05:59Z
dc.date.issued2021
dc.description.abstractThe first water soluble, bisphosphonate (BP, not bisphosphonic acid)-functionalized poly(amido amine) macromer (PAA-BP) is synthesized and used as a crosslinker for synthesis of a biodegradable and biocompatible hydrogel for tissue engineering scaffolds. The synthesis of PAA-BP is performed in three steps, the first two giving the control macromers (PAA-NHBoc and PAA-NH2): i) Michael addition reaction of N,N'-methylene bisacrylamide and N-Boc-1,6-hexanediamine (acrylamide/amine ratios of 1.2), ii) deprotection of Boc-protected amine groups, iii) Michael addition reaction of the amine groups with tetraethyl vinylidene bisphosphonate. The degree of BP substitution is 50% and molecular weight of the PAA-BP macromer is found to be 4800 g/mol. These macromers are incorporated into hydrogels by copolymerization with 2-hydroxyethyl methacrylate and the influence of bisphosphonate functionality on hydrogel properties; degradation, swelling, mechanical and mineralization, is investigated. The mineralization abilities, hence the mechanical properties of the hydrogels are strongly influenced by the BP functionality; PAA-BP forming strong (E = 83 +/- 1 kPa) hydrogel-apatite composites, PAA-NH2 also working to a lesser degree (E = 54 +/- 3 kPa). Cytocompatibility of the hydrogels is observed on Saos-2 human osteosarcoma, U-2 OS human bone osteosarcoma epithelial, C2C12 mouse myoblast muscle and NIH mouse embryonic fibroblast 3T3 cells. PAA-BP crosslinked hydrogels facilitate adhesion of C2C12 cells after mineralization. In summary, BP-functionalized hydrogel may have a potential impact on bone tissue engineering.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.volume159
dc.identifier.doi10.1016/j.eurpolymj.2021.110732
dc.identifier.eissn1873-1945
dc.identifier.issn0014-3057
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85114044974
dc.identifier.urihttp://dx.doi.org/10.1016/j.eurpolymj.2021.110732
dc.identifier.urihttps://hdl.handle.net/20.500.14288/8891
dc.identifier.wos696979000003
dc.keywordsHydrogels
dc.keywordsBisphosphonates
dc.keywordsPoly(amido amine)s
dc.keywordsBiomineralization
dc.keywordsCrosslinker
dc.languageEnglish
dc.publisherElsevier
dc.sourceEuropean Polymer Journal
dc.subjectPolymer science
dc.titleBisphosphonate-functionalized poly(amido amine) crosslinked 2-hydrox-yethyl methacrylate hydrogel as tissue engineering scaffold
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0003-4952-7981
local.contributor.authorid0000-0001-5601-8814
local.contributor.kuauthorDemirci, Gözde
local.contributor.kuauthorAcar, Havva Funda Yağcı
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Chemistry
local.publication.orgunit2Graduate School of Sciences and Engineering
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