Publication: High prevalence of ArmA-16S rRNA methyltransferase among aminoglycoside-resistant Klebsiella pneumoniae bloodstream isolates
dc.contributor.coauthor | Isler, Burcu | |
dc.contributor.coauthor | Falconer, Caitlin | |
dc.contributor.coauthor | Çınar, Güle | |
dc.contributor.coauthor | Aslan, Abdullah Tarık | |
dc.contributor.coauthor | Forde, Brian | |
dc.contributor.coauthor | Harris, Patrick | |
dc.contributor.coauthor | Şimşek, Funda | |
dc.contributor.coauthor | Tülek, Necla | |
dc.contributor.coauthor | Demirkaya, Hamiyet | |
dc.contributor.coauthor | Menekşe, Şirin | |
dc.contributor.coauthor | Akalin, Halis | |
dc.contributor.coauthor | Balkan, İlker İnanç | |
dc.contributor.coauthor | Aydın, Mehtap | |
dc.contributor.coauthor | Tigen, Elif Tükenmez | |
dc.contributor.coauthor | Demir, Safiye Koçulu | |
dc.contributor.coauthor | Arabacı, Çiğdem | |
dc.contributor.coauthor | Yağcı, Serap | |
dc.contributor.coauthor | Hazırolan, Gülşen | |
dc.contributor.coauthor | Gönen, Mehmet | |
dc.contributor.coauthor | Saltoğlu, Neşe | |
dc.contributor.coauthor | Azap, Özlem | |
dc.contributor.coauthor | Akova, Murat | |
dc.contributor.coauthor | Paterson, David L. | |
dc.contributor.department | KUISCID (Koç University İşbank Center for Infectious Diseases) | |
dc.contributor.department | KUH (Koç University Hospital) | |
dc.contributor.department | Graduate School of Health Sciences | |
dc.contributor.department | Graduate School of Sciences and Engineering | |
dc.contributor.department | School of Medicine | |
dc.contributor.kuauthor | Bakır, Veli Oğuzalp | |
dc.contributor.kuauthor | Can, Füsun | |
dc.contributor.kuauthor | Doğan, Özlem | |
dc.contributor.kuauthor | Kapmaz, Mahir | |
dc.contributor.kuauthor | Keske, Şiran | |
dc.contributor.kuauthor | Özer, Berna | |
dc.contributor.kuauthor | Vatansever, Cansel | |
dc.contributor.kuauthor | Ergönül, Önder | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF HEALTH SCIENCES | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
dc.contributor.schoolcollegeinstitute | KUH (KOÇ UNIVERSITY HOSPITAL) | |
dc.contributor.schoolcollegeinstitute | Research Center | |
dc.contributor.schoolcollegeinstitute | SCHOOL OF MEDICINE | |
dc.date.accessioned | 2024-11-09T23:51:10Z | |
dc.date.issued | 2022 | |
dc.description.abstract | Introduction. Aminoglycosides are used for the treatment of carbapenemase-producing Klebsiella pneumoniae (CPK) infections. 16S rRNA methyltransferases (RMTs) confer resistance to all aminoglycosides and are often cocarried with NDM. Hypothesis/Gap Statement. There is a dart of studies looking at the aminoglycoside resistance mechanisms for invasive CPK isolates, particularly in OXA-48 endemic settings. Aim. We aimed to determine the prevalence of RMTs and their association with beta lactamases and MLSTs amongst aminoglycoside-resistant CPK bloodstream isolates in an OXA-48 endemic setting. Methodology. CPK isolates (n=181), collected as part of a multicentre cohort study, were tested for amikacin, gentamicin and tobramycin susceptibility using custom-made sensititre plates (GN2XF, Thermo Fisher Scientific). All isolates were previously subjected to whole-genome sequencing. Carbapenemases, RMTs, MLSTs and plasmid incompatibility groups were detected on the assembled genomes. Results. Of the 181 isolates, 109(60 %) were resistant to all three aminoglycosides, and 96 of 109(88 %) aminoglycoside-resistant isolates carried an RMT (85 ArmA, 10 RmtC, 4 RmtF1; three isolates cocarried ArmA and RmtC). Main clonal types associated with ArmA were ST2096 (49/85, 58 %) and ST14 (24/85, 28 %), harbouring mainly OXA-232 and OXA-48 +NDM, respectively. RmtC was cocarried with NDM (5/10) on ST395, and NDM +OXA-48 or NDM +KPC (4/10) on ST14, ST15 and ST16. All RMT producers also carried CTX-M-15, and the majority cocarried SHV-106, TEM-150 and multiple other antibiotic resistance genes. The majority of the isolates harboured a combination of IncFIB, IncH and IncL/M type plasmids. Non-NDM producing isolates remained susceptible to ceftazidime-avibactam. Conclusion. Aminoglycoside resistance amongst CPK bloodstream isolates is extremely common and mainly driven by clonal spread of ArmA carried on ST2096 and ST14, associated with OXA-232 and OXA48 +NDM carriage, respectively. | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 12 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.volume | 71 | |
dc.identifier.doi | 10.1099/jmm.0.001629 | |
dc.identifier.issn | 1473-5644 | |
dc.identifier.scopus | 2-s2.0-85147460921 | |
dc.identifier.uri | https://doi.org/10.1099/jmm.0.001629 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/14664 | |
dc.identifier.wos | 1129579900012 | |
dc.keywords | 16S rRNA methyltransferase | |
dc.keywords | Aminoglycoside resistance | |
dc.keywords | ArmA | |
dc.keywords | Bloodstream | |
dc.keywords | Carbapenem-resistant | |
dc.keywords | Klebsiella pneumoniae | |
dc.keywords | NDM | |
dc.keywords | OXA-232 | |
dc.keywords | OXA-48 | |
dc.keywords | Aminoglycoside | |
dc.keywords | Antiinfective agent | |
dc.keywords | Bacterial protein | |
dc.keywords | Beta lactamase | |
dc.keywords | Methyltransferase | |
dc.keywords | RNA 16S | |
dc.keywords | Carbapenem-resistant enterobacteriaceae | |
dc.keywords | Cohort analysis | |
dc.keywords | Genetics | |
dc.keywords | Human | |
dc.keywords | Klebsiella infection | |
dc.keywords | Klebsiella pneumoniae | |
dc.keywords | Microbial sensitivity test | |
dc.keywords | Prevalence | |
dc.keywords | Aminoglycosides | |
dc.keywords | Anti-bacterial agents | |
dc.keywords | Bacterial proteins | |
dc.keywords | Beta-lactamases | |
dc.keywords | Carbapenem-resistant enterobacteriaceae | |
dc.keywords | Cohort studies | |
dc.keywords | Humans | |
dc.keywords | Klebsiella infections | |
dc.keywords | Klebsiella pneumoniae | |
dc.keywords | Methyltransferases | |
dc.keywords | Microbial sensitivity tests | |
dc.keywords | Prevalence | |
dc.keywords | RNA, Ribosomal, 16S | |
dc.language.iso | eng | |
dc.publisher | Microbiology Society | |
dc.relation.ispartof | Journal of medical microbiology | |
dc.subject | Beta-lactamases | |
dc.subject | Carbapenem-resistant enterobacteriaceae | |
dc.subject | Klebsiella pneumoniae | |
dc.title | High prevalence of ArmA-16S rRNA methyltransferase among aminoglycoside-resistant Klebsiella pneumoniae bloodstream isolates | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Keske, Şiran | |
local.contributor.kuauthor | Doğan, Özlem | |
local.contributor.kuauthor | Ergönül, Mehmet Önder | |
local.contributor.kuauthor | Can, Füsun | |
local.contributor.kuauthor | Kapmaz, Mahir | |
local.contributor.kuauthor | Özer, Berna | |
local.contributor.kuauthor | Vatansever, Cansel | |
local.contributor.kuauthor | Bakır, Veli Oğuzalp | |
local.publication.orgunit1 | SCHOOL OF MEDICINE | |
local.publication.orgunit1 | GRADUATE SCHOOL OF HEALTH SCIENCES | |
local.publication.orgunit1 | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
local.publication.orgunit1 | KUH (KOÇ UNIVERSITY HOSPITAL) | |
local.publication.orgunit1 | Research Center | |
local.publication.orgunit2 | KUISCID (Koç University İşbank Center for Infectious Diseases) | |
local.publication.orgunit2 | KUH (Koç University Hospital) | |
local.publication.orgunit2 | School of Medicine | |
local.publication.orgunit2 | Graduate School of Health Sciences | |
local.publication.orgunit2 | Graduate School of Sciences and Engineering | |
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