Publication: The effects of genistein supplementation on fructose induced insulin resistance, oxidative stress and inflammation
Program
KU-Authors
KU Authors
Co-Authors
Bolayirli, I. Murat
Inan, Oznur
Aydin, M. Serif
Bilgin, I. Ahmet
Sayan, Ismet
Esrefoglu, Mukaddes
Seven, Arzu
Advisor
Publication Date
2016
Language
English
Type
Journal Article
Journal Title
Journal ISSN
Volume Title
Abstract
Aims: This experimental study was designed to investigate the effects of 10 weeks genistein administration on oxidative stress and inflammation in serum and liver of rats fed with fructose. Main methods: 6-8 weeks old, 40 male Sprague-Dawley rats were included. Group 1 (control) was fed with standard chow food and 100 1/kg/day/rat dimethyl sulfoxide (DMSO) administered subcutaneously; group 2 (genistein) with standard chow food and 025 mg/kg/day/rat genistein; group 3 (fructose) with standard chow food and drinking water 20% fructose, group 4 (fructose + genistein) with standard chow food, drinking water with 20% fructose and 025 mg/kg/day/rat genistein. TNF-alpha, IL-6, visfatin as inflammatory markers and 8-isoprostane as a oxidative stress marker were measured by ELISA, glucose, triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol by enzymatic calorimetric method, AST and ALT by kinetic UV method. Key findings: Significantly high 8-isoprostane levels in serum (p < 0.001) and liver (p < 0.05) in group 3 compared to control group indicate that presence of oxidative stress. Significantly high TNF-alpha and IL-6 levels in serum (p < 0.05) and liver (p < 0.01) and visfatin levels in serum (p < 0.001) of group 3 indicate inflammation accompanying insulin resistance and oxidative stress. Genistein administration to fructose group causes a significant decrease in HOMA-IR (p < 0.001) and LDLC (p < 0.05) level. Significantly lower serum 8-isoprostane (p < 0.01) level indicates the antioxidant effect of genistein and significantly lower liver TNF-alpha (p < 0.01), serum, liver IL-6(p < 0.01) and serum visfatin (p < 0.01) levels reflect the antiinflammatory effects of genistein. Significance: Genistein administration to rats fed with fructose causes an ameliorating effect on HOMA-IR values and lipid status markers in addition to its antioxidant and antiinflammatory effects.
Description
Source:
Life Sciences
Publisher:
Elsevier
Keywords:
Subject
Medicine, research and experimentall, Pharmacology and pharmacy