Publication:
Effect of varying magnetic fields on targeted gene delivery of nucleic acid-based molecules

dc.contributor.coauthorOral, Özlem
dc.contributor.coauthorCikim, Taha
dc.contributor.coauthorZuvin, Merve
dc.contributor.coauthorGözüaçık, Devrim
dc.contributor.coauthorKoşar, Ali
dc.contributor.departmentN/A
dc.contributor.departmentDepartment of Chemistry
dc.contributor.kuauthorÜnal, Özlem
dc.contributor.kuauthorAcar, Havva Funda Yağcı
dc.contributor.kuprofilePhD Student
dc.contributor.kuprofileFaculty Member
dc.contributor.otherDepartment of Chemistry
dc.contributor.schoolcollegeinstituteGraduate School of Sciences and Engineering
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.yokidN/A
dc.contributor.yokid178902
dc.date.accessioned2024-11-09T22:57:19Z
dc.date.issued2015
dc.description.abstractSeveral physical methods have been developed to introduce nucleic acid expression vectors into mammalian cells. Magnetic transfection (magnetofection) is one such transfection method, and it involves binding of nucleic acids such as DNA, RNA or siRNA to magnetic nanoparticles followed by subsequent exposure to external magnetic fields. However, the challenge between high efficiency of nucleic acid uptake by cells and toxicity was not totally resolved. Delivery of nucleic acids and their transport to the target cells require carefully designed and controlled systems. In this study, we introduced a novel magnetic system design providing varying magnet turn speeds and magnetic field directions. The system was tested in the magnetofection of human breast (MCF-7), prostate (DU-145, PC-3) and bladder (RT-4) cancer cell lines using green fluorescent protein DNA as a reporter. Polyethylenimine coated superparamagnetic iron oxide nanoparticles (SPIONs) were used as nucleic acid carriers. Adsorption of PEI on SPION improved the cytocompatibility dramatically. Application of external magnetic field increased intracellular uptake of nanoparticles and transfection efficiency without any additional cytotoxicity. We introduce our novel magnetism-based method as a promising tool for enhanced nucleic acid delivery into mammalian cells.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue11
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsorshipTurkish Scientific Council (TUBITAK) [112M875]
dc.description.sponsorshipSabanci University
dc.description.sponsorshipTurkish Academy of Sciences
dc.description.sponsorshipEMBO-SDIG Award
dc.description.sponsorshipTurkish Academy of Sciences (TUBA-GEBIP) Award The authors would like to thank the Sabanci University Nanotechnology Research and Application Center (SUNUM) and Koc University Surface Science and Technology Center (KUYTAM) for the continued equipment and characterization support. This work was supported by Turkish Scientific Council (TUBITAK), grant number: 112M875, Sabanci University and Turkish Academy of Sciences. Graduate student support provided by the Faculty of Engineering and Natural Sciences of Sabanci University is greatly appreciated. D.G. is a recipient of EMBO-SDIG Award. A.K. received TUBITAKIncentive Award. D.G. and A.K. are recipients of Turkish Academy of Sciences (TUBA-GEBIP) Award. H.Y.A received National L'Oreal Women in Science Reward in Materials Science.
dc.description.volume43
dc.identifier.doi10.1007/s10439-015-1331-6
dc.identifier.eissn1573-9686
dc.identifier.issn0090-6964
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-84945454958
dc.identifier.urihttp://dx.doi.org/10.1007/s10439-015-1331-6
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7533
dc.identifier.wos363238800019
dc.keywordsFerrofluid
dc.keywordsMagnetic actuation
dc.keywordsVertical and horizontal magnetic field
dc.keywordsPolyethyleneimine (PEI)
dc.keywordsSuper-paramagnetic iron oxide nanoparticles (SPIONs)
dc.keywordsMagnetofection
dc.keywordsGreen fluorescent protein (GFP)-DNA
dc.languageEnglish
dc.publisherSpringer
dc.sourceAnnals of Biomedical Engineering
dc.subjectEngineering
dc.subjectBiomedical engineering
dc.titleEffect of varying magnetic fields on targeted gene delivery of nucleic acid-based molecules
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authoridN/A
local.contributor.authorid0000-0001-5601-8814
local.contributor.kuauthorÜnal, Özlem
local.contributor.kuauthorAcar, Havva Funda Yağcı
relation.isOrgUnitOfPublication035d8150-86c9-4107-af16-a6f0a4d538eb
relation.isOrgUnitOfPublication.latestForDiscovery035d8150-86c9-4107-af16-a6f0a4d538eb

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