Publication: Effect of varying magnetic fields on targeted gene delivery of nucleic acid-based molecules
dc.contributor.coauthor | Oral, Özlem | |
dc.contributor.coauthor | Cikim, Taha | |
dc.contributor.coauthor | Zuvin, Merve | |
dc.contributor.coauthor | Gözüaçık, Devrim | |
dc.contributor.coauthor | Koşar, Ali | |
dc.contributor.department | N/A | |
dc.contributor.department | Department of Chemistry | |
dc.contributor.kuauthor | Ünal, Özlem | |
dc.contributor.kuauthor | Acar, Havva Funda Yağcı | |
dc.contributor.kuprofile | PhD Student | |
dc.contributor.kuprofile | Faculty Member | |
dc.contributor.other | Department of Chemistry | |
dc.contributor.schoolcollegeinstitute | Graduate School of Sciences and Engineering | |
dc.contributor.schoolcollegeinstitute | College of Sciences | |
dc.contributor.yokid | N/A | |
dc.contributor.yokid | 178902 | |
dc.date.accessioned | 2024-11-09T22:57:19Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Several physical methods have been developed to introduce nucleic acid expression vectors into mammalian cells. Magnetic transfection (magnetofection) is one such transfection method, and it involves binding of nucleic acids such as DNA, RNA or siRNA to magnetic nanoparticles followed by subsequent exposure to external magnetic fields. However, the challenge between high efficiency of nucleic acid uptake by cells and toxicity was not totally resolved. Delivery of nucleic acids and their transport to the target cells require carefully designed and controlled systems. In this study, we introduced a novel magnetic system design providing varying magnet turn speeds and magnetic field directions. The system was tested in the magnetofection of human breast (MCF-7), prostate (DU-145, PC-3) and bladder (RT-4) cancer cell lines using green fluorescent protein DNA as a reporter. Polyethylenimine coated superparamagnetic iron oxide nanoparticles (SPIONs) were used as nucleic acid carriers. Adsorption of PEI on SPION improved the cytocompatibility dramatically. Application of external magnetic field increased intracellular uptake of nanoparticles and transfection efficiency without any additional cytotoxicity. We introduce our novel magnetism-based method as a promising tool for enhanced nucleic acid delivery into mammalian cells. | |
dc.description.indexedby | WoS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 11 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | International | |
dc.description.sponsorship | Turkish Scientific Council (TUBITAK) [112M875] | |
dc.description.sponsorship | Sabanci University | |
dc.description.sponsorship | Turkish Academy of Sciences | |
dc.description.sponsorship | EMBO-SDIG Award | |
dc.description.sponsorship | Turkish Academy of Sciences (TUBA-GEBIP) Award The authors would like to thank the Sabanci University Nanotechnology Research and Application Center (SUNUM) and Koc University Surface Science and Technology Center (KUYTAM) for the continued equipment and characterization support. This work was supported by Turkish Scientific Council (TUBITAK), grant number: 112M875, Sabanci University and Turkish Academy of Sciences. Graduate student support provided by the Faculty of Engineering and Natural Sciences of Sabanci University is greatly appreciated. D.G. is a recipient of EMBO-SDIG Award. A.K. received TUBITAKIncentive Award. D.G. and A.K. are recipients of Turkish Academy of Sciences (TUBA-GEBIP) Award. H.Y.A received National L'Oreal Women in Science Reward in Materials Science. | |
dc.description.volume | 43 | |
dc.identifier.doi | 10.1007/s10439-015-1331-6 | |
dc.identifier.eissn | 1573-9686 | |
dc.identifier.issn | 0090-6964 | |
dc.identifier.quartile | Q2 | |
dc.identifier.scopus | 2-s2.0-84945454958 | |
dc.identifier.uri | http://dx.doi.org/10.1007/s10439-015-1331-6 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/7533 | |
dc.identifier.wos | 363238800019 | |
dc.keywords | Ferrofluid | |
dc.keywords | Magnetic actuation | |
dc.keywords | Vertical and horizontal magnetic field | |
dc.keywords | Polyethyleneimine (PEI) | |
dc.keywords | Super-paramagnetic iron oxide nanoparticles (SPIONs) | |
dc.keywords | Magnetofection | |
dc.keywords | Green fluorescent protein (GFP)-DNA | |
dc.language | English | |
dc.publisher | Springer | |
dc.source | Annals of Biomedical Engineering | |
dc.subject | Engineering | |
dc.subject | Biomedical engineering | |
dc.title | Effect of varying magnetic fields on targeted gene delivery of nucleic acid-based molecules | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.authorid | N/A | |
local.contributor.authorid | 0000-0001-5601-8814 | |
local.contributor.kuauthor | Ünal, Özlem | |
local.contributor.kuauthor | Acar, Havva Funda Yağcı | |
relation.isOrgUnitOfPublication | 035d8150-86c9-4107-af16-a6f0a4d538eb | |
relation.isOrgUnitOfPublication.latestForDiscovery | 035d8150-86c9-4107-af16-a6f0a4d538eb |