Publication:
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

dc.contributor.departmentKUYTAM (Koç University Surface Science and Technology Center)
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorKhodadust, Rouhollah
dc.contributor.kuauthorAcar, Havva Funda Yağcı
dc.contributor.kuauthorÜnal, Özlem
dc.contributor.kuprofilePhD Student
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.yokidN/A
dc.contributor.yokid178902
dc.contributor.yokidN/A
dc.date.accessioned2024-11-09T12:41:15Z
dc.date.issued2022
dc.description.abstractPolyethylenimine (PEI), which is frequently used for polyplex formation and effective gene transfection, is rarely recognized as a luminescent polymer. Therefore, it is usually tagged with an organic fluorophore to be optically tracked. Recently, we developed branched PEI (bPEI) superparamagnetic iron oxide nanoparticles (SPION@bPEI) with blue luminescence 1200 times stronger than that of bPEI without a traditional fluorophore, due to partial PEI oxidation during the synthesis. Here, we demonstrate in vitro dye free optical imaging and successful gene transfection with luminescent SPION@bPEI, which was further modified for receptor mediated delivery of the cargo selectively to cancer cell lines overexpressing the epidermal growth factor receptor (EGFR). Proapoptotic polyinosinic-polycytidylic acid sodium (PIC) was delivered to HeLa cells with SPION@bPEI and caused a dramatic reduction in the cell viability at otherwise non-toxic nanoparticle concentrations, proving that bPEI coating is still an effective component for the delivery of an anionic cargo. Besides, a strong intracellular optical signal supports the optically traceable nature of these nanoparticles. SPION@bPEI nanoparticles were further conjugated with Erbitux (Erb), which is an anti-EGFR antibody for targeting EGFR-overexpressing cancer cell lines. SPION@bPEI-Erb was used for the delivery of a GFP plasmid wherein the transfection was confirmed by the luminescence of the expressed gene within the transfected cells. Poor GFP expression in MCF7, a slightly better expression in HeLa, and a significant enhancement in the transfection of HCT116 cells proved a selective uptake and hence the targeting ability of Erb-tagged nanoparticles. Altogether, this study proves luminescent, cationic, and small SPION@bPEI nanoparticles as strong candidates for imaging and gene therapy.
dc.description.fulltextYES
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipKoç University
dc.description.versionPublisher version
dc.description.volume13
dc.formatpdf
dc.identifier.doi10.3762/bjnano.13.6
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR03469
dc.identifier.issn2190-4286
dc.identifier.linkhttps://doi.org/10.3762/bjnano.13.6
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-85123823778
dc.identifier.urihttps://hdl.handle.net/20.500.14288/2240
dc.identifier.wos744651800001
dc.keywordsErbitux
dc.keywordsPhotoluminescence
dc.keywordsPolyethyleneimine
dc.keywordsPolyinosinic-polycytidylic acid sodium
dc.keywordsSuperparamagnetic iron oxide nanoparticles
dc.languageEnglish
dc.publisherBeilstein-Institut
dc.relation.grantnoNA
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/10263
dc.sourceBeilstein Journal of Nanotechnology
dc.subjectNanoscience and nanotechnology
dc.subjectMaterials science
dc.subjectPhysics
dc.titleTheranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authoridN/A
local.contributor.authorid0000-0001-5601-8814
local.contributor.authoridN/A
local.contributor.kuauthorKhodadust, Rouhollah
local.contributor.kuauthorAcar, Havva Funda Yağcı
local.contributor.kuauthorÜnal, Özlem
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1Research Center
local.publication.orgunit2KUYTAM (Koç University Surface Science and Technology Center)
local.publication.orgunit2Graduate School of Sciences and Engineering
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