Publication: Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae
dc.contributor.coauthor | Rahbarghazi, Reza | |
dc.contributor.coauthor | Saberianpour, Shirin | |
dc.contributor.coauthor | Delkhosh, Aref | |
dc.contributor.coauthor | Amini, Hassan | |
dc.contributor.coauthor | Hassanpour, Mehdi | |
dc.contributor.department | KUTTAM (Koç University Research Center for Translational Medicine) | |
dc.contributor.department | Graduate School of Health Sciences | |
dc.contributor.department | School of Medicine | |
dc.contributor.kuauthor | Heidarzadeh, Morteza | |
dc.contributor.kuauthor | Sokullu, Emel | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF HEALTH SCIENCES | |
dc.contributor.schoolcollegeinstitute | Research Center | |
dc.contributor.schoolcollegeinstitute | SCHOOL OF MEDICINE | |
dc.date.accessioned | 2024-11-09T13:25:44Z | |
dc.date.issued | 2021 | |
dc.description.abstract | Objective: the current experiment aimed to assess the impact of detergents such as 3% Triton X-100, 1% peracetic acid, 1% Tween-20, and 1% SDS in combination with Trypsin–EDTA on acellularization of ovine aortae after 7 days. Results: Hematoxylin–Eosin staining showed an appropriate acellularization rate in ovine aortae, indicated by a lack of cell nuclei in the tunica media layer. DAPI staining confirmed the lack of nuclei in the vascular wall after being exposed to the combination of chemical and enzymatic solutions. Verhoeff-Van Gieson staining showed that elastin fibers were diminished in acellular samples compared to the control group while collagen stands were unchanged. CCK-8 survival assay showed enhanced viability in human umbilical vein endothelial cells 5 days after being cultured on decellularized samples compared to the cells cultured on a plastic surface (p < 0.05). SEM imaging showed flattening of endothelial cells on the acellular surface. | |
dc.description.fulltext | YES | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 1 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.sponsorship | Tabriz University of Medical Sciences | |
dc.description.version | Publisher version | |
dc.description.volume | 14 | |
dc.identifier.doi | 10.1186/s13104-021-05538-3 | |
dc.identifier.embargo | NO | |
dc.identifier.filenameinventoryno | IR02826 | |
dc.identifier.issn | 1756-0500 | |
dc.identifier.quartile | N/A | |
dc.identifier.scopus | 2-s2.0-85104045082 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/3459 | |
dc.identifier.wos | 638001100001 | |
dc.keywords | Acellularization | |
dc.keywords | Attachment | |
dc.keywords | Endothelial cells | |
dc.keywords | Ovine aorta | |
dc.keywords | Survival rate | |
dc.language.iso | eng | |
dc.publisher | BioMed Central | |
dc.relation.grantno | NA | |
dc.relation.ispartof | BMC Research Notes | |
dc.relation.uri | http://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/9477 | |
dc.subject | Life sciences | |
dc.subject | Biomedicine | |
dc.subject | Science and technology | |
dc.subject | Biology | |
dc.subject | Multidisciplinary sciences | |
dc.title | Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Heidarzadeh, Morteza | |
local.contributor.kuauthor | Sokullu, Emel | |
local.publication.orgunit1 | GRADUATE SCHOOL OF HEALTH SCIENCES | |
local.publication.orgunit1 | SCHOOL OF MEDICINE | |
local.publication.orgunit1 | Research Center | |
local.publication.orgunit2 | KUTTAM (Koç University Research Center for Translational Medicine) | |
local.publication.orgunit2 | School of Medicine | |
local.publication.orgunit2 | Graduate School of Health Sciences | |
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