Publication:
Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae

dc.contributor.coauthorRahbarghazi, Reza
dc.contributor.coauthorSaberianpour, Shirin
dc.contributor.coauthorDelkhosh, Aref
dc.contributor.coauthorAmini, Hassan
dc.contributor.coauthorHassanpour, Mehdi
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorHeidarzadeh, Morteza
dc.contributor.kuauthorSokullu, Emel
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T13:25:44Z
dc.date.issued2021
dc.description.abstractObjective: the current experiment aimed to assess the impact of detergents such as 3% Triton X-100, 1% peracetic acid, 1% Tween-20, and 1% SDS in combination with Trypsin–EDTA on acellularization of ovine aortae after 7 days. Results: Hematoxylin–Eosin staining showed an appropriate acellularization rate in ovine aortae, indicated by a lack of cell nuclei in the tunica media layer. DAPI staining confirmed the lack of nuclei in the vascular wall after being exposed to the combination of chemical and enzymatic solutions. Verhoeff-Van Gieson staining showed that elastin fibers were diminished in acellular samples compared to the control group while collagen stands were unchanged. CCK-8 survival assay showed enhanced viability in human umbilical vein endothelial cells 5 days after being cultured on decellularized samples compared to the cells cultured on a plastic surface (p < 0.05). SEM imaging showed flattening of endothelial cells on the acellular surface.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipTabriz University of Medical Sciences
dc.description.versionPublisher version
dc.description.volume14
dc.identifier.doi10.1186/s13104-021-05538-3
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR02826
dc.identifier.issn1756-0500
dc.identifier.quartileN/A
dc.identifier.scopus2-s2.0-85104045082
dc.identifier.urihttps://hdl.handle.net/20.500.14288/3459
dc.identifier.wos638001100001
dc.keywordsAcellularization
dc.keywordsAttachment
dc.keywordsEndothelial cells
dc.keywordsOvine aorta
dc.keywordsSurvival rate
dc.language.isoeng
dc.publisherBioMed Central
dc.relation.grantnoNA
dc.relation.ispartofBMC Research Notes
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/9477
dc.subjectLife sciences
dc.subjectBiomedicine
dc.subjectScience and technology
dc.subjectBiology
dc.subjectMultidisciplinary sciences
dc.titleDistinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorHeidarzadeh, Morteza
local.contributor.kuauthorSokullu, Emel
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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