Publication: Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography
dc.contributor.coauthor | Dao, E. Han | |
dc.contributor.coauthor | Su, Zhen | |
dc.contributor.coauthor | Poitevin, Frederic | |
dc.contributor.coauthor | Yoon, Chun Hong | |
dc.contributor.coauthor | Kupitz, Christopher | |
dc.contributor.coauthor | Hayes, Brandon | |
dc.contributor.coauthor | Liang, Mengning | |
dc.contributor.coauthor | Hunter, Mark S. | |
dc.contributor.coauthor | Batyuk, Alexander | |
dc.contributor.coauthor | Sierra, Raymond G. | |
dc.contributor.coauthor | Ketawala, Gihan | |
dc.contributor.coauthor | Botha, Sabine | |
dc.contributor.department | Department of Molecular Biology and Genetics | |
dc.contributor.department | Graduate School of Sciences and Engineering | |
dc.contributor.department | KUISCID (Koç University İşbank Center for Infectious Diseases) | |
dc.contributor.kuauthor | Ayan, Esra | |
dc.contributor.kuauthor | Büyükdağ, Cengizhan | |
dc.contributor.kuauthor | Çakılkaya, Barış | |
dc.contributor.kuauthor | Dağ, Çağdaş | |
dc.contributor.kuauthor | Demirci, Hasan | |
dc.contributor.kuauthor | Ertem, Fatma Betül | |
dc.contributor.kuauthor | Göcenler, Oktay | |
dc.contributor.kuauthor | Gül, Mehmet | |
dc.contributor.kuauthor | Güven, Ömür | |
dc.contributor.kuauthor | Johnson, Jerome Austin | |
dc.contributor.kuauthor | Karakadıoğlu, Gözde Usta | |
dc.contributor.kuauthor | Yüksel, Büşra | |
dc.contributor.schoolcollegeinstitute | College of Sciences | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
dc.contributor.schoolcollegeinstitute | Research Center | |
dc.date.accessioned | 2024-11-09T13:19:33Z | |
dc.date.issued | 2022 | |
dc.description.abstract | The SARS-CoV-2 main protease of (Mpro) is an important target for SARS-CoV-2 related drug repurposing and development studies. Here, we describe the steps for structural characterization of SARS-CoV-2 Mpro, starting from plasmid preparation and protein purification. We detail the steps for crystallization using the sitting drop, microbatch (under oil) approach. Finally, we cover data collection and structure determination using serial femtosecond crystallography. | |
dc.description.fulltext | YES | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 1 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | TÜBİTAK | |
dc.description.sponsorship | National Science Foundation (NSF) Science and Technology Centers | |
dc.description.sponsorship | BioXFEL | |
dc.description.sponsorship | National Institutes of Health (NIH) | |
dc.description.sponsorship | National Institute of General Medical Sciences (NIGMS) | |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TÜBİTAK) | |
dc.description.sponsorship | 2232 International Fellowship for Outstanding Researchers Program of TÜBİTAK | |
dc.description.sponsorship | 2244 Industrial PhD Program of TÜBİTAK | |
dc.description.sponsorship | 1001 TÜBİTAK Supporting Scientific and Technological Research Projects Program | |
dc.description.sponsorship | Linac Coherent Light Source (LCLS) | |
dc.description.sponsorship | Stanford Synchrotron Light Source (SSRL) | |
dc.description.sponsorship | SLAC National Accelerator Laboratory | |
dc.description.sponsorship | U.S. Department of Energy, Office of Science, Basic Energy Sciences | |
dc.description.version | Publisher version | |
dc.description.volume | 3 | |
dc.identifier.doi | 10.1016/j.xpro.2022.101158 | |
dc.identifier.embargo | NO | |
dc.identifier.filenameinventoryno | IR03558 | |
dc.identifier.issn | 2666-1667 | |
dc.identifier.quartile | N/A | |
dc.identifier.scopus | 2-s2.0-85124088015 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/3131 | |
dc.keywords | Protein biochemistry | |
dc.keywords | Protein expression and purification | |
dc.keywords | Structural biology | |
dc.keywords | X-ray crystallography | |
dc.language.iso | eng | |
dc.publisher | Cell Press | |
dc.relation.grantno | NSF-1231306 | |
dc.relation.grantno | 118C270 | |
dc.relation.grantno | 119C132 | |
dc.relation.grantno | 120Z52 | |
dc.relation.grantno | DE-AC02-76SF0051 | |
dc.relation.grantno | DE-AC02-76SF00515 | |
dc.relation.grantno | P41GM103393 | |
dc.relation.ispartof | Star Protocols | |
dc.relation.uri | http://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/10416 | |
dc.subject | X-Ray laser | |
dc.subject | Crystallography | |
dc.subject | Serials | |
dc.title | Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Ertem, Fatma Betül | |
local.contributor.kuauthor | Güven, Ömür | |
local.contributor.kuauthor | Büyükdağ, Cengizhan | |
local.contributor.kuauthor | Göcenler, Oktay | |
local.contributor.kuauthor | Ayan, Esra | |
local.contributor.kuauthor | Yüksel, Büşra | |
local.contributor.kuauthor | Gül, Mehmet | |
local.contributor.kuauthor | Karakadıoğlu, Gözde Usta | |
local.contributor.kuauthor | Çakılkaya, Barış | |
local.contributor.kuauthor | Johnson, Jerome Austin | |
local.contributor.kuauthor | Demirci, Hasan | |
local.contributor.kuauthor | Dağ, Çağdaş | |
local.publication.orgunit1 | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
local.publication.orgunit1 | College of Sciences | |
local.publication.orgunit1 | Research Center | |
local.publication.orgunit2 | KUISCID (Koç University İşbank Center for Infectious Diseases) | |
local.publication.orgunit2 | Department of Molecular Biology and Genetics | |
local.publication.orgunit2 | Graduate School of Sciences and Engineering | |
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