Publication:
Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography

Simple item page
dc.contributor.coauthorDao, E. Han
dc.contributor.coauthorSu, Zhen
dc.contributor.coauthorPoitevin, Frederic
dc.contributor.coauthorYoon, Chun Hong
dc.contributor.coauthorKupitz, Christopher
dc.contributor.coauthorHayes, Brandon
dc.contributor.coauthorLiang, Mengning
dc.contributor.coauthorHunter, Mark S.
dc.contributor.coauthorBatyuk, Alexander
dc.contributor.coauthorSierra, Raymond G.
dc.contributor.coauthorKetawala, Gihan
dc.contributor.coauthorBotha, Sabine
dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentKUISCID (Koç University İşbank Center for Infectious Diseases)
dc.contributor.kuauthorAyan, Esra
dc.contributor.kuauthorBüyükdağ, Cengizhan
dc.contributor.kuauthorÇakılkaya, Barış
dc.contributor.kuauthorDağ, Çağdaş
dc.contributor.kuauthorDemirci, Hasan
dc.contributor.kuauthorErtem, Fatma Betül
dc.contributor.kuauthorGöcenler, Oktay
dc.contributor.kuauthorGül, Mehmet
dc.contributor.kuauthorGüven, Ömür
dc.contributor.kuauthorJohnson, Jerome Austin
dc.contributor.kuauthorKarakadıoğlu, Gözde Usta
dc.contributor.kuauthorYüksel, Büşra
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-11-09T13:19:33Z
dc.date.issued2022
dc.description.abstractThe SARS-CoV-2 main protease of (Mpro) is an important target for SARS-CoV-2 related drug repurposing and development studies. Here, we describe the steps for structural characterization of SARS-CoV-2 Mpro, starting from plasmid preparation and protein purification. We detail the steps for crystallization using the sitting drop, microbatch (under oil) approach. Finally, we cover data collection and structure determination using serial femtosecond crystallography.
dc.description.fulltextYES
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuTÜBİTAK
dc.description.sponsorshipNational Science Foundation (NSF) Science and Technology Centers
dc.description.sponsorshipBioXFEL
dc.description.sponsorshipNational Institutes of Health (NIH)
dc.description.sponsorshipNational Institute of General Medical Sciences (NIGMS)
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TÜBİTAK)
dc.description.sponsorship2232 International Fellowship for Outstanding Researchers Program of TÜBİTAK
dc.description.sponsorship2244 Industrial PhD Program of TÜBİTAK
dc.description.sponsorship1001 TÜBİTAK Supporting Scientific and Technological Research Projects Program
dc.description.sponsorshipLinac Coherent Light Source (LCLS)
dc.description.sponsorshipStanford Synchrotron Light Source (SSRL)
dc.description.sponsorshipSLAC National Accelerator Laboratory
dc.description.sponsorshipU.S. Department of Energy, Office of Science, Basic Energy Sciences
dc.description.versionPublisher version
dc.description.volume3
dc.identifier.doi10.1016/j.xpro.2022.101158
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR03558
dc.identifier.issn2666-1667
dc.identifier.quartileN/A
dc.identifier.scopus2-s2.0-85124088015
dc.identifier.urihttps://hdl.handle.net/20.500.14288/3131
dc.keywordsProtein biochemistry
dc.keywordsProtein expression and purification
dc.keywordsStructural biology
dc.keywordsX-ray crystallography
dc.language.isoeng
dc.publisherCell Press
dc.relation.grantnoNSF-1231306
dc.relation.grantno118C270
dc.relation.grantno119C132
dc.relation.grantno120Z52
dc.relation.grantnoDE-AC02-76SF0051
dc.relation.grantnoDE-AC02-76SF00515
dc.relation.grantnoP41GM103393
dc.relation.ispartofStar Protocols
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/10416
dc.subjectX-Ray laser
dc.subjectCrystallography
dc.subjectSerials
dc.titleProtocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorErtem, Fatma Betül
local.contributor.kuauthorGüven, Ömür
local.contributor.kuauthorBüyükdağ, Cengizhan
local.contributor.kuauthorGöcenler, Oktay
local.contributor.kuauthorAyan, Esra
local.contributor.kuauthorYüksel, Büşra
local.contributor.kuauthorGül, Mehmet
local.contributor.kuauthorKarakadıoğlu, Gözde Usta
local.contributor.kuauthorÇakılkaya, Barış
local.contributor.kuauthorJohnson, Jerome Austin
local.contributor.kuauthorDemirci, Hasan
local.contributor.kuauthorDağ, Çağdaş
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit1Research Center
local.publication.orgunit2KUISCID (Koç University İşbank Center for Infectious Diseases)
local.publication.orgunit2Department of Molecular Biology and Genetics
local.publication.orgunit2Graduate School of Sciences and Engineering
relation.isOrgUnitOfPublicationaee2d329-aabe-4b58-ba67-09dbf8575547
relation.isOrgUnitOfPublication3fc31c89-e803-4eb1-af6b-6258bc42c3d8
relation.isOrgUnitOfPublication09525e58-d4ea-4461-b2ec-f131e54c0771
relation.isOrgUnitOfPublication.latestForDiscoveryaee2d329-aabe-4b58-ba67-09dbf8575547
relation.isParentOrgUnitOfPublicationaf0395b0-7219-4165-a909-7016fa30932d
relation.isParentOrgUnitOfPublication434c9663-2b11-4e66-9399-c863e2ebae43
relation.isParentOrgUnitOfPublicationd437580f-9309-4ecb-864a-4af58309d287
relation.isParentOrgUnitOfPublication.latestForDiscoveryaf0395b0-7219-4165-a909-7016fa30932d

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
10416.pdf
Size:
4.91 MB
Format:
Adobe Portable Document Format
Download

Collections

Publications with Fulltext