Publication: Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography
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Program
KU Authors
Co-Authors
Dao, E. Han
Su, Zhen
Poitevin, Frederic
Yoon, Chun Hong
Kupitz, Christopher
Hayes, Brandon
Liang, Mengning
Hunter, Mark S.
Batyuk, Alexander
Sierra, Raymond G.
Advisor
Publication Date
2022
Language
English
Type
Journal Article
Journal Title
Journal ISSN
Volume Title
Abstract
The SARS-CoV-2 main protease of (Mpro) is an important target for SARS-CoV-2 related drug repurposing and development studies. Here, we describe the steps for structural characterization of SARS-CoV-2 Mpro, starting from plasmid preparation and protein purification. We detail the steps for crystallization using the sitting drop, microbatch (under oil) approach. Finally, we cover data collection and structure determination using serial femtosecond crystallography.
Description
Source:
Star Protocols
Publisher:
Cell Press
Keywords:
Subject
X-Ray laser, Crystallography, Serials