Publication: Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography
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KU Authors
Co-Authors
Dao, E. Han
Su, Zhen
Poitevin, Frederic
Yoon, Chun Hong
Kupitz, Christopher
Hayes, Brandon
Liang, Mengning
Hunter, Mark S.
Batyuk, Alexander
Sierra, Raymond G.
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English
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Abstract
The SARS-CoV-2 main protease of (Mpro) is an important target for SARS-CoV-2 related drug repurposing and development studies. Here, we describe the steps for structural characterization of SARS-CoV-2 Mpro, starting from plasmid preparation and protein purification. We detail the steps for crystallization using the sitting drop, microbatch (under oil) approach. Finally, we cover data collection and structure determination using serial femtosecond crystallography.
Source:
Star Protocols
Publisher:
Cell Press
Keywords:
Subject
X-Ray laser, Crystallography, Serials