Publication:
Ruthenium-induced corneal collagen crosslinking under visible light

dc.contributor.departmentDepartment of Mechanical Engineering
dc.contributor.departmentDepartment of Chemical and Biological Engineering
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorGülzar, Ayesha
dc.contributor.kuauthorKaleli, Humeyra Nur
dc.contributor.kuauthorKızılel, Seda
dc.contributor.kuauthorLazoğlu, İsmail
dc.contributor.kuauthorMalik, Anjum Naeem
dc.contributor.kuauthorNazeer, Muhammad Anwaar
dc.contributor.kuauthorŞahin, Afsun
dc.contributor.kuauthorTaş, Ayşe Yıldız
dc.contributor.kuauthorYıldız, Erdost
dc.contributor.kuauthorZibandeh, Noushin
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T23:10:33Z
dc.date.issued2022
dc.description.abstractCorneal collagen crosslinking (CXL) is a commonly used minimally invasive surgical technique to prevent the progression of corneal ectasias, such as keratoconus. Unfortunately, riboflavin/UV-A light-based CXL procedures have not been successfully applied to all patients, and result in frequent complications, such as corneal haze and endothelial damage. We propose a new method for corneal crosslinking by using a Ruthenium (Ru) based water-soluble photoinitiator and visible light (430 nm). Tris(bipyridine)ruthenium(II) ([Ru(bpy)3]2+) and sodium persulfate (SPS) mixture covalently crosslinks free tyrosine, histidine, and lysine groups under visible light (400-450 nm), which prevents UV-A light-induced cytotoxicity in an efficient and time saving collagen crosslinking procedure. In this study, we investigated the effects of the Ru/visible blue light procedure on the viability and toxicity of human corneal epithelium, limbal, and stromal cells. Then bovine corneas crosslinked with ruthenium mixture and visible light were characterized, and their biomechanical properties were compared with the customized riboflavin/UV-A crosslinking approach in the clinics. Crosslinked corneas with a ruthenium-based CXL approach showed significantly higher young's modulus compared to riboflavin/UV-A light-based method applied to corneas. In addition, crosslinked corneas with both methods were characterized to evaluate the hydrodynamic behavior, optical transparency, and enzymatic resistance. In all biomechanical, biochemical, and optical tests used here, corneas that were crosslinked with ruthenium-based approach demonstrated better results than that of corneas crosslinked with riboflavin/ UV-A. This study is promising to be translated into a non-surgical therapy for all ectatic corneal pathologies as a result of mild conditions introduced here with visible light exposure and a nontoxic ruthenium-based photoinitiator to the cornea. STATEMENT OF SIGNIFICANCE: Keratoconus, one of the most frequent corneal diseases, could be treated with riboflavin and ultraviolet light-based photo-crosslinking application to the cornea of the patients. Unfortunately, this method has irreversible side effects and cannot be applied to all keratoconus patients. In this study, we exploited the photoactivation behavior of an organoruthenium compound to achieve corneal crosslinking. Ruthenium-based organic complex under visible light demonstrated significantly better biocompatibility and superior biomechanical results than riboflavin and ultraviolet light application. This study promises to translate into a new fast, efficient non-surgical therapy option for all ectatic corneal pathologies.
dc.description.indexedbyWOS
dc.description.indexedbyPubMed
dc.description.issue7
dc.description.openaccessNO
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipTUBITAK [ARDEB-219S349] TUBITAK ARDEB-219S349
dc.description.volume63
dc.identifier.eissn1552-5783
dc.identifier.issn0146-0404
dc.identifier.scopus2-s2.0-85132419773
dc.identifier.urihttps://hdl.handle.net/20.500.14288/9480
dc.identifier.wos848054000012
dc.language.isoeng
dc.publisherAssoc Research Vision Ophthalmology Inc
dc.relation.ispartofInvestigative Ophthalmology & Visual Science
dc.subjectOphthalmology
dc.titleRuthenium-induced corneal collagen crosslinking under visible light
dc.typeMeeting Abstract
dspace.entity.typePublication
local.contributor.kuauthorYıldız, Erdost
local.contributor.kuauthorGülzar, Ayesha
local.contributor.kuauthorKaleli, Humeyra Nur
local.contributor.kuauthorNazeer, Muhammad Anwaar
local.contributor.kuauthorZibandeh, Noushin
local.contributor.kuauthorMalik, Anjum Naeem
local.contributor.kuauthorTaş, Ayşe Yıldız
local.contributor.kuauthorLazoğlu, İsmail
local.contributor.kuauthorŞahin, Afsun
local.contributor.kuauthorKızılel, Seda
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1College of Engineering
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Mechanical Engineering
local.publication.orgunit2Department of Chemical and Biological Engineering
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
local.publication.orgunit2Graduate School of Sciences and Engineering
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