Publication:
Cytotoxicity and mitogenicity assays with real-time and label-free monitoring of human granulosa cells with an impedance-based signal processing technology intergrating micro-electronics and cell biology

dc.contributor.coauthorYakar, Feridun
dc.contributor.coauthorGüzel, Yılmaz
dc.contributor.coauthorBalaban, Başak
dc.contributor.coauthorIwase, Akira
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.kuauthorAkın, Nazlı
dc.contributor.kuauthorBildik, Gamze
dc.contributor.kuauthorLack, Nathan Alan
dc.contributor.kuauthorÖktem, Özgür
dc.contributor.kuauthorŞenbabaoğlu, Filiz
dc.contributor.kuauthorUrman, Cumhur Bülent
dc.contributor.kuauthorUrman, Defne
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T23:04:20Z
dc.date.issued2016
dc.description.abstractA recently developed technology (xCelligence) integrating micro-electronics and cell biology allows real-time, uninterrupted and quantitative analysis of cell proliferation, viability and cytotoxicity by measuring the electrical impedance of the cell population in the wells without using any labeling agent. In this study we investigated if this system is a suitable model to analyze the effects of mitogenic (FSH) and cytotoxic (chemotherapy) agents with different toxicity profiles on human granulosa cells in comparison to conventional methods of assessing cell viability, DNA damage, apoptosis and steroidogenesis. The system generated the real-time growth curves of the cells, and determined their doubling times, mean cell indices and generated dose-response curves after exposure to cytotoxic and mitogenic stimuli. It accurately predicted the gonadotoxicity of the drugs and distinguished less toxic agents (5-FU and paclitaxel) from more toxic ones (cisplatin and cyclophosphamide). This platform can be a useful tool for specific end-point assays in reproductive toxicology.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume60
dc.identifier.doi10.1016/j.reprotox.2015.12.005
dc.identifier.issn0890-6238
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-84958191715
dc.identifier.urihttps://doi.org/10.1016/j.reprotox.2015.12.005
dc.identifier.urihttps://hdl.handle.net/20.500.14288/8622
dc.identifier.wos376225400010
dc.keywordsXcelligence
dc.keywordsChemotherapy
dc.keywordsCytotoxicity
dc.keywordsHuman granulosa cells
dc.keywordsMitogenicity
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofReproductive Toxicology
dc.subjectReproductive biology
dc.subjectToxicology
dc.titleCytotoxicity and mitogenicity assays with real-time and label-free monitoring of human granulosa cells with an impedance-based signal processing technology intergrating micro-electronics and cell biology
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorÖktem, Özgür
local.contributor.kuauthorBildik, Gamze
local.contributor.kuauthorŞenbabaoğlu, Filiz
local.contributor.kuauthorLack, Nathan Alan
local.contributor.kuauthorAkın, Nazlı
local.contributor.kuauthorUrman, Defne
local.contributor.kuauthorUrman, Cumhur Bülent
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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