Publication:
Purification and characterization of five members of photolyase/cryptochrome family from cyanidioschyzon merolae

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dc.contributor.departmentN/A
dc.contributor.departmentDepartment of Chemical and Biological Engineering
dc.contributor.kuauthorAsımgil, Hande
dc.contributor.kuauthorKavaklı, İbrahim Halil
dc.contributor.kuprofilePhD Student
dc.contributor.kuprofileFaculty Member
dc.contributor.otherDepartment of Chemical and Biological Engineering
dc.contributor.schoolcollegeinstituteGraduate School of Sciences and Engineering
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.yokidN/A
dc.contributor.yokid40319
dc.date.accessioned2024-11-09T23:43:16Z
dc.date.issued2012
dc.description.abstractThe photolyase/cryptochrome family is a large family of flavoproteins that possess different functions and use blue light as an energy source. Photolyases repair UV-induced DNA damage, whereas cryptochromes regulate the growth and development of plants in a blue-light dependent manner. In this paper, we report the characterization of five genes the photolyase/cryptochrome family from the red algae Cyanidioschyzon merolae. Phylogenetic analysis indicated that one gene is close to the (6-4) photolyase, 3 to the cryptochrome-dash (CRY-DASH), and one gene is an independent clade. We investigated the diversity and similarity of the enzymes' biochemical and photochemical properties. Both biochemical and complementation assays indicated that one of the CRY-DASH genes (CmPHR6) is not involved in the repair of either ssDNA or dsDNA. In addition, we isolated the first known (6-4) photolyase from C. merolae, the most primitive photosynthetic organism, which will give evolutionary insights into this protein family. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessNO
dc.description.sponsorshipTUBITAK [110T938]
dc.description.sponsorshipTUBA-GEBIP This work was supported by TUBITAK under grant 110T938. IHK acknowledges support from the TUBA-GEBIP program. HA thanks TUBITAK 2214 BIDEB. We would like to thank Dr. Cory Dunn and Zeynep Ali for critically reading this manuscript.
dc.description.volume185
dc.identifier.doi10.1016/j.plantsci.2011.10.005
dc.identifier.eissn1873-2259
dc.identifier.issn0168-9452
dc.identifier.scopus2-s2.0-84856681083
dc.identifier.urihttp://dx.doi.org/10.1016/j.plantsci.2011.10.005
dc.identifier.urihttps://hdl.handle.net/20.500.14288/13463
dc.identifier.wos301218300021
dc.keywordsPhotolyase
dc.keywordsCryptochrome
dc.keywords(6-4) Photolyase
dc.keywordsCry-dash
dc.keywordsDNA repair
dc.keywordsCyanidioschyzon merolae
dc.keywordsBlue-light Photoreceptors
dc.keywordsUnicellular red alga
dc.keywordsDNA Photolyase
dc.keywordsEscherichia-coli
dc.keywordsCryptochrome/photolyase family
dc.keywordsCrystal-structure
dc.keywordsRepair
dc.keywordsIdentification
dc.keywordsReceptors
dc.keywordsEvolution
dc.languageEnglish
dc.publisherElsevier Ireland Ltd
dc.sourcePlant Science
dc.subjectBiochemistry
dc.subjectMolecular biology
dc.subjectPlant sciences
dc.titlePurification and characterization of five members of photolyase/cryptochrome family from cyanidioschyzon merolae
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authoridN/A
local.contributor.authorid0000-0001-6624-3505
local.contributor.kuauthorAsımgil, Hande
local.contributor.kuauthorKavaklı, İbrahim Halil
relation.isOrgUnitOfPublicationc747a256-6e0c-4969-b1bf-3b9f2f674289
relation.isOrgUnitOfPublication.latestForDiscoveryc747a256-6e0c-4969-b1bf-3b9f2f674289

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